Journal: iScience

Article Title: The potential of Protaetia brevitarsis as a functional food that enhances immune function and gut microbiota in mice

doi: 10.1016/j.isci.2026.114939

Figure Lengend Snippet: Effect of PB on immunity of mice CK, 100% basal diet; PB1, 5% PB powder + 95% basal diet; PB2, 10% PB powder + 90% basal diet; PB3, 20% PB powder + 80% basal diet. (A) The transformation value of T lymphocytes converting into lymphoblasts upon stimulation with ConA in vitro . (B) The degree of inflammatory response in the foot sole of mice after injection of SRBC. (C) The phagocytic index (α) of macrophages in the liver and spleen for carbon particles. (D) Phagocytosis index of chicken erythrocytes by peritoneal macrophages. (E) Phagocytosis percentage of chicken erythrocytes by peritoneal macrophages. (F) The content of hemolysin in the serum. It is expressed as the half value of hemolysin (HC50). (G) The content of IgG in the serum. (H) The content of IgA in the serum. (I) The content of TNF-α in the serum. (J) NK cell activity. Expressed by the amount of LDH released by YAC-1 cells after being killed by NK cells. Each experiment is conducted independently and does not interfere with the others. Each experiment has 10 biological replicates and 3 technical replicates. Data are presented as mean ± SE. Statistical significance was determined by one-way ANOVA with multiple comparisons; ∗ p ≤ 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001 versus the CK group.

Article Snippet: YAC-1 , Procell , Cat# CL-0246; RRID:CVCL_2244.

Techniques: Transformation Assay, In Vitro, Injection, Activity Assay

Journal: International Journal of Molecular Sciences

Article Title: CD127 + Natural Killer Cells Represent a Distinct, Interleukin-15-Independent and Thymus-Independent Subset in Mice

doi: 10.3390/ijms27062667

Figure Lengend Snippet: Functional characterization of murine CD127 + NK cells. ( a ) Cytotoxic activity of sorted, freshly isolated CD127 + and CD127 − NK cells from the spleen and lymph nodes of adult C57BL/6 mice ( n = 5) was assessed against YAC-1 target cells using a 51 Cr-release assay at the indicated effector-to-target (E/T) ratios. CD127 + NK cells exhibited measurable cytotoxic activity; however, their lytic capacity was significantly lower than that of CD127 − NK cells at multiple E/T ratios. ( b ) Intracellular IFN-γ production by CD127 + and CD127 − NK cells following stimulation with anti-NK1.1 mAb (PK136). Representative flow cytometric plots are shown, with numbers in the quadrants indicating the percentage of cells in each region. Data are presented as mean ± SD. Statistical comparisons between CD127 + and CD127 − subsets were performed using an unpaired two-tailed Student’s t -test. * p < 0.05, ** p < 0.01. IFN-γ—interferon-γ; mAb—monoclonal antibody.

Article Snippet: Briefly, NK cell-mediated cytotoxicity was assessed by measuring 51 Cr-release following the incubation of freshly isolated, unstimulated effector cells with 51 Cr-labeled YAC-1 target cells (ATCC).

Techniques: Functional Assay, Activity Assay, Isolation, Release Assay, Two Tailed Test