Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis

doi: 10.1007/978-1-0716-0175-4_2

Figure Lengend Snippet: Published antibodies for Xenopus eye

Article Snippet: Rod photoreceptor cells , Anti-XAP-2, clone 5B9 , Developmental Studies Hybridoma Bank, AB_528087 , 1:25 , [ 40 , 44 ].

Techniques: Membrane, Marker

Journal: Methods in molecular biology (Clifton, N.J.)

Article Title: Studying In Vivo Retinal Progenitor Cell Proliferation in Xenopus laevis

doi: 10.1007/978-1-0716-0175-4_2

Figure Lengend Snippet: Published antibodies for Xenopus eye

Article Snippet: Rod photoreceptor cells , Anti-XAP-2, clone 5B9 , Developmental Studies Hybridoma Bank, AB_528087 , 1:25 , [ 40 , 44 ].

Techniques: Membrane, Marker

Journal: Frontiers in Molecular Neuroscience

Article Title: The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

doi: 10.3389/fnmol.2023.1160707

Figure Lengend Snippet: MNU-mediated injury predominantly affects rods, UV-cones and the outer plexiform layer. Schematics of photoreceptor organization in a transversal section (A) and frontal flattened view (B) , based on , , and . INL, inner nuclear layer; rONL, rod outer nuclear layer; cONL, cone outer nuclear layer; OPL, outer plexiform layer; RPE, retinal pigmented epithelium; DC, double cones (red/green spectral sensitivity); LC, long single cones (blue spectral sensitivity); SC, short single cone (UV spectral sensitivity). (C) Ubiquitin-promoter driven loxP-EGFP-loxP-mCherry transgene is expressed in blue and UV cones. Top panel shows a schematic illustration and the bottom panel displays a section of uninjured retina with ubi:EGFP transgene expression (green) and DAPI (blue). (D) Transversal retinal section stained with 4C12 antibody to visualize cell bodies and inner segments of rods (green), Phalloidin to detect F-actin (red) and DAPI (blue). In uninjured retina, colocalization between 4C12 and Phalloidin (arrows) is detected at the level of inner segments. F-actin is also present in the photoreceptor processes in the outer plexiform layer (outlined with a dashed lines). At 5 dpMNU, F-actin in the outer plexiform layer is missing and rod cells bodies and their inner segments are disorganized. (E) Identification of Xap-2 antibody (red) as a marker of photoreceptor outer segments on section of ubi:EGFP transgenic retinas. A strong expression is detected distally to the oil droplet in the UV cones (purple asterisks) and blue cones (cyan asterisks). (F) Identification of Xap-1 antibody (red) as a marker of the cone outer segment on section of ubi:EGFP transgenic retinas. A dotty localization of Xap1 is enriched distally to the oil droplet in the UV cones (purple asterisks). Dots of Xap-1 labeling are also observed in the outer segments of other photoreceptors, but at much less concentrated level. N ≥ 3.

Article Snippet: The following primary antibodies were used: mouse anti glutamine synthetase (GS) at 1:200 (MAB302; Millipore, Billerica, MA, USA), (provided by Enzmann V. Group, University of Bern), mouse anti-proliferating cell nuclear antigen (PCNA) at 1:200 (Clone PC10; M0879; DAKO), rabbit anti-MCM5 at 1:500 (kindly provided by Soojin Ryu, Heidelberg); mouse IgG ZRF-1/GFAP 1:100 (ZIRC, University of Oregon), mouse IgG ZPR-1 1:100 (ZIRC, University of Oregon), mouse IgM Xap-1 (Clone 3D2) at 1:10 (DSHB), mouse IgG3 Xap-2 (Clone 5B9) at 1:10 (DSHB), mouse anti Vimentin at 1:50 (40E-C, DSHB) Chick anti-GFP antibody at 1:500 (GFP-1020; Aves labs); Rabbit anti KO2 at 1:200 (PM051M, MBL International Corporation).

Techniques: Expressing, Staining, Marker, Transgenic Assay, Labeling

Journal: Frontiers in Molecular Neuroscience

Article Title: The regeneration-responsive element careg monitors activation of Müller glia after MNU-induced damage of photoreceptors in the zebrafish retina

doi: 10.3389/fnmol.2023.1160707

Figure Lengend Snippet: Restoration of rods and UV cones after MNU-injury. (A) Schematic illustration and sections of intact and 30 dpMNU retinas immunostained against the rod marker 4C12 (green), the F-actin marker Phalloidin (red) and DAPI (blue). The distribution of rods (4C12, green) and synaptic processes of the OPL (encircled with a dashed line) are restored at 30 dpMNU. (B) Schematic illustration and sections of intact and 40 dpMNU retinas immunostained with Xap-2 (green), Xap-1 (red) antibodies, and DAPI (blue). The outer segment of UV-cones is restored at 40 dpMNU. INL, inner nuclear layer; OPL, outer plexiform layer; rONL, rod outer nuclear layer; cONL, cone outer nuclear layer. N = 3.

Article Snippet: The following primary antibodies were used: mouse anti glutamine synthetase (GS) at 1:200 (MAB302; Millipore, Billerica, MA, USA), (provided by Enzmann V. Group, University of Bern), mouse anti-proliferating cell nuclear antigen (PCNA) at 1:200 (Clone PC10; M0879; DAKO), rabbit anti-MCM5 at 1:500 (kindly provided by Soojin Ryu, Heidelberg); mouse IgG ZRF-1/GFAP 1:100 (ZIRC, University of Oregon), mouse IgG ZPR-1 1:100 (ZIRC, University of Oregon), mouse IgM Xap-1 (Clone 3D2) at 1:10 (DSHB), mouse IgG3 Xap-2 (Clone 5B9) at 1:10 (DSHB), mouse anti Vimentin at 1:50 (40E-C, DSHB) Chick anti-GFP antibody at 1:500 (GFP-1020; Aves labs); Rabbit anti KO2 at 1:200 (PM051M, MBL International Corporation).

Techniques: Marker