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Journal: Scientific Reports
Article Title: Microfluidic mixing probe: generating multiple concentration-varying flow dipoles
doi: 10.1038/s41598-025-85797-3
Figure Lengend Snippet: Design and experimental setup of the MMP system. (a) Schematic of the MMP design showing an isometric, see-through view. The MMP includes two injection channels (Injection 1 and Injection 2) and an aspiration channel, with mixing channels located below the holder key. The base of the MMP features a central mesa where fluidic interactions occur. The inset shows a top-down view of the mesa with injection and aspiration regions. (b) Diagram of the experimental setup, including the XYZ micropositioner, syringe pump, and MMP. Syringe pump channels are labeled for aspiration, Injection 1 (molecule, 100%), and Injection 2 (buffer, 0%). These injections create distinct concentration-varying flow dipoles of biochemical concentrations that are confined under the MMP mesa due to aspiration and the surrounding immersion fluid. Inset: The concentration gradient HFC profiles (100%, 50%, 0%) generated on adherent cells within the Petri dish. (c) Simulation of concentration gradient profiles formed by the mixing of the molecule (100%) and buffer (0%) within the MMP channels. The left panel shows the mixing process creating a 50% concentration in the middle channel, while the right panel shows the full setup with the aspiration and injection mechanisms confining the flow. (d) The micrograph shows the experimental setup, consisting of inverted fluorescence microscope, syringe pump equipped with microfluidic syringes, 3D-printed XYZ micropositioner, and 3D-printed MMP. The micrograph in the inset shows Injection 1, Injection 2, and aspiration directions through the MMP.
Article Snippet: The MMP also incorporates a custom-designed, 3D-printed twist-lock assembly that secures it to a
Techniques: Injection, Labeling, Concentration Assay, Generated, Fluorescence, Microscopy