Journal: Bioactive Materials

Article Title: Dual-engineering metalloimmunotherapy mediates Staphylococcus aureus virulence silencing and biofilm immune microenvironment reprogramming against implant-associated infections

doi: 10.1016/j.bioactmat.2026.03.008

Figure Lengend Snippet: Critical roles of bacterial virulence and host cGAS–STING signaling in IAIs. a , Schematic workflow of clinical S. aureus isolation from orthopedic patients with IAIs and subsequent laboratory virulence assessment. b , Correlation analysis of patient serum PCT levels and the hemolysis ability of infected S. aureus . c , Kaplan-Meier analysis showing the correlation of S. aureus virulence and patient infection-free survival. d , Representative X-ray and PET/CT images of patients infected with high- or low-virulence S. aureus strains. e , Experimental workflow of the murine knee periprosthetic joint infection (PJI) model. f , g , Colony-forming unit (CFU) counts of viable bacteria in peri-implant infected tissues ( f ) and extracted implants ( g ) from PJI model mice on day 14. h , Representative micro-CT images of tibiae from PJI model mice on day 28. i , Representative 3D reconstructed images of gait footprints from PJI model mice. j , o , Experimental workflow of the murine single ( j ) and mixed ( o ) infection IAIs models. k , l , p , q , CFU counts of viable bacteria in peri-implant infected tissues ( k , p ) and extracted implants ( l , q ) from IAIs model mice. m , r , Representative H&E and Giemsa staining images of peri-implant infected tissues from mice on day 14. m , r , Representative Confocal laser scanning microscopy (CLSM) 3D reconstruction images of biofilms on extracted implants from mice on day 14. t , Representative bioluminescence images of mice on day 14. u-z , Representative culture images and corresponding CFU counts of viable bacteria in peri-implant infected tissues and extracted implants from mixed infection IAIs model mice (WT-lux + Δ saeRS group, u-w ; WT + Δ saeRS -lux group, x-z ). The yellow arrow indicates the Δ saeRS colonies. Data are presented as mean ± s.d. from n biologically independent samples ( f , g , k , l , p , q , u , v , x and y , n = 5). Statistical significance was analyzed by two-way ANOVA with Tukey's test for f , g , k , l , p and q and two-tailed paired t tests for u , v , x and y .

Article Snippet: To evaluate the therapeutic efficacy of HMPF nanoparticles against S. aureus -induced osteolysis, X-ray imaging (XRD; Rigaku Ultima IV) was performed on PJI mice on day 28, followed by micro-CT scanning (Skyscan 1172, Bruker Micro-CT, Germany) and quantitative analysis of the tibiae.

Techniques: Isolation, Infection, Positron Emission Tomography-Computed Tomography, Bacteria, Micro-CT, Staining, Confocal Laser Scanning Microscopy, Two Tailed Test

Journal: Bioactive Materials

Article Title: Dual-engineering metalloimmunotherapy mediates Staphylococcus aureus virulence silencing and biofilm immune microenvironment reprogramming against implant-associated infections

doi: 10.1016/j.bioactmat.2026.03.008

Figure Lengend Snippet: HMPF nanoparticles exhibit excellent therapeutic efficacy in treating mice with S. aureus -indu ced PJI. a , Experimental workflow for evaluating the therapeutic efficacy against knee prosthetic joint infection (PJI) of mice in vivo. b , c , Individual ( b ) and average ( c ) infection area growth curves of PJI model mice in different treatment groups. d , Bioluminescence intensity curves of PJI model mice in different groups on days −2, 2, 6, 10, and 14. e , f , CFU counts of viable bacteria in the peri-implant infected tissues ( e ) and extracted implants ( f ) from PJI model mice after different treatments on day 14. g - k , Representative micro-CT and X-ray images ( k ) and quantification of bone volume/total volume (BV/TV) ( g ), cancellous bone mineral density (BMD) ( h ), trabecular thickness (TB. TH) ( i ) and joint angle ( j ). l , Schematic illustration of the gait analysis experiment. m , Representative footprint images of PJI mice in different groups on day 14. n - q , Quantification of gait analysis data, including support time ( n ), stride length ( o ), average intensity (average pressure on the runway) ( p ), and average speed ( q ). r , Representative 3D reconstructed images of left hind footprints from PJI mice in different treatment groups. The color bar represents the pressure intensity of each part of the mouse footprint on the ground. Data are presented as mean ± s.d. from n biologically independent samples ( b and c , n = 6; d and g - j , n = 3; e , f and n - q , n = 8). Statistical significance was analyzed by one-way ANOVA with Tukey's multiple comparisons test for e - j and n - q .

Article Snippet: To evaluate the therapeutic efficacy of HMPF nanoparticles against S. aureus -induced osteolysis, X-ray imaging (XRD; Rigaku Ultima IV) was performed on PJI mice on day 28, followed by micro-CT scanning (Skyscan 1172, Bruker Micro-CT, Germany) and quantitative analysis of the tibiae.

Techniques: Drug discovery, Infection, In Vivo, Bacteria, Micro-CT

Journal: Nature Communications

Article Title: Experimental demonstration of corrugated nanolaminate films as reflective light sails

doi: 10.1038/s41467-026-73414-4

Figure Lengend Snippet: a Fabrication steps for sail film prototypes in the indented trench configuration (see Methods; for information on scaling this process to larger suspended sizes, see Supplementary Note ). b Scanning electron micrograph (SEM) and energy dispersive X-ray spectroscopy (EDS) images of multilayer film on Si substrate. The presence of color in the images corresponds to the presence of the indicated element. See Supplementary Fig. for the associated spectrum. c Experimentally-measured normal-incidence reflectivity ( ϱ λ ,⊥ ) and transmissivity ( τ λ ,⊥ ) spectra for fabricated prototype film, along with resulting absorptivity ( α λ ,⊥ = 1 − ϱ λ ,⊥ − τ λ ,⊥ ) spectrum. Shaded areas for ϱ λ ,⊥ and τ λ ,⊥ reflect a 3% uncertainty along with the maximum variation among three sets of measurements (see Supplementary Note for a detailed description). Black dash/dot lines show simulated spectra obtained using the transfer-matrix method with complex indices of refraction obtained from ellipsometry measurements (see Methods and Supplementary Note ). d Simulated spectrum for proposed optimized film, calculated using indices of refraction obtained from the literature , . Shaded gray regions in ( c ) and ( d ) denote the laser band. e Calculated bending stiffness enhancement factor of hexagonally corrugated structures relative to their planar (non-corrugated) counterparts as a function of the ratio of the hexagon diameter to trench width ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\frac{{d}_{h}}{{w}_{t}}$$\end{document} d h w t , abscissa) and the ratio of the trench height to the total composite film thickness ( \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\frac{{h}_{t}}{{t}_{u}}$$\end{document} h t t u , ordinate). Circle marks fabricated prototype film and triangle marks feasible proposed optimized film (see Supplementary Notes and ). Prototype film dimensions: d h ≈ 77 μm (measured flat-to-flat), w t ≈ 15 μm, h t ≈ 10 μm, t A , b ≈ 21 nm (bottom Al 2 O thickness), t M ≈ 53 nm (MoS 2 ), t A , t ≈ 51 nm (top Al 2 O ), ρ a , c ≈ 0.7 g ⋅ m −2 (corrugated film areal density). Proposed optimized film dimensions: d h = 70 μm, w t = 2 μm, h t = 3 μm, t A , b = t A , t ≈ 19 nm, t M ≈ 63 nm, ρ a , c ≈ 0.5 g ⋅ m −2 . We define the total/unified film thickness to be t u = t A , b + t M + t A , t . Scale bar: ( b ) 20 μm.

Article Snippet: We acquired our scanning electron micrographs (SEM) and energy dispersive X-ray images using a TESCAN S8000X machine.

Techniques: Spectroscopy