Journal: Annals of Indian Academy of Neurology

Article Title: Clinical Application of Circulating MicroRNAs in Parkinson's Disease: The Challenges and Opportunities as Diagnostic Biomarker

doi: 10.4103/aian.AIAN_440_19

Figure Lengend Snippet: Proposed candidate miRNAs in various body fluids of PD compared to healthy controls

Article Snippet: Khoo et al ., 2012 , Plasma , , TRI reagent RT-blood protocol (Molecular Research Center, Cincinnati, OH) , , Saint Mary’s Health Care Hauenstein Parkinson’s Center (SMHCPC), Grand Rapids, MI, USA , PD-32 HC-32 , Agilent whole human genome miRNA microarray v. 3 [Agilent, Santa Clara, CA)] Validated with StepOnePlus RT-PCR system ]Applied Biosystems, Foster City, CA] , miR-222, miR-626, and miR-505 , , ( ) .

Techniques: Isolation, Derivative Assay, TLDA Assay, Spectrophotometry, Electrophoresis, Hybridization, Real-time Polymerase Chain Reaction, Microarray, Quantitative RT-PCR, Fluorescence, Protein Extraction, Reverse Transcription Polymerase Chain Reaction, Sequencing, Next-Generation Sequencing

Journal: Stem Cell Research & Therapy

Article Title: Genome-wide identification of long noncoding RNAs and their competing endogenous RNA networks involved in the odontogenic differentiation of human dental pulp stem cells

doi: 10.1186/s13287-020-01622-w

Figure Lengend Snippet: Flow chart of ceRNA regulatory network construction. First, Arraystar Human LncRNA Expression Array V4.0 and the Whole Human miRNA microarray were used to detect differentially expressed lncRNAs, miRNAs, and mRNAs. Second, 4 odontogenesis-related mRNAs were screened by literature. Third, target mRNAs of miRNAs were predicted using TargetScan and miRanda, and target lncRNAs of differentially expressed miRNAs were predicted using miRcode. Then, differentially expressed lncRNAs and mRNAs were merged with the target lncRNAs and mRNAs of differentially expressed miRNAs, respectively. The co-expression lncRNAs and mRNAs were selected (fold change > 2 and P < 0.05). Finally, the differentially expressed miRNAs, co-expression lncRNAs, and mRNAs were mapped into the interactions. DERNAs, differentially expressed RNAs; ceRNA, competitive endogenous RNA; lncRNA, long noncoding RNA; miRNA, microRNA; mRNA, messenger RNA

Article Snippet: The Whole Human microRNA (miRNA) microarray (KangChen Bio-tech, Shanghai, China) was used to perform expression profiling of all known miRNAs in the human transcriptome.

Techniques: Expressing, Microarray

Journal: Data in Brief

Article Title: Placental microRNA dataset of monochorionic twin pregnancies with and without selective fetal growth restriction

doi: 10.1016/j.dib.2020.105403

Figure Lengend Snippet:

Article Snippet: Description of data collection , The placental miRNAs were extracted by miRNeasy mini kit and profiled by Agilent Sure Print Whole Genome Human miRNA microarrays. MiRNA features was extracted by Agilent Gene Spring GX software..

Techniques: Expressing, Microarray, Software

Journal: International Journal of Molecular Sciences

Article Title: Role of MicroRNAs in Parkinson’s Disease

doi: 10.3390/ijms20225649

Figure Lengend Snippet: Summary of 15 miRNAs reported to be dysregulated in the brain/neuronal model(s), cerebrospinal fluid (CSF) and blood.

Article Snippet: , hsa-miR-200a , ↑ , - , - , Plasma from 32 PD patients vs. 32 controls , Agilent whole human genome miRNA microarray v.3 , [ ] .

Techniques: RNA Sequencing Assay, Isolation, TLDA Assay, Real-time Polymerase Chain Reaction, Microarray, Transgenic Assay, Expressing, Next-Generation Sequencing