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Benchling Inc web-based sequence designer
Web Based Sequence Designer, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/web-based+sequence+designer/web+based+sequence+designer/pm39498890-240-12-14
Average 90 stars, based on 1 article reviews
web-based sequence designer - by Bioz Stars, 2026-09
90/100 stars

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Plasmid Preparation:

Article Title: A model-driven approach towards rational microbial bioprocess optimization.
Article Snippet: Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products.. Nevertheless, the nonhomogenous variations of extracellular environment in bioprocesses often challenge the biomass growth and the bioproduction yield.. To enable a more rational bioprocess optimization, we have established a model-driven approach which systematically integrates experiments with modelling, executed from flask to bioreactor scale, using ferulic acid to vanillin bioconversion as a case study.

Article Title: Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae.
Article Snippet: New strategies to control cholera are urgently needed.. This study develops an in vitro proof-ofconcept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach.. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein (YebF-Art-085), thereby self-lysing to release the killing protein (Art-085) to kill V. cholerae.

Article Title: Cell-Free Optogenetic Gene Expression System.
Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Optimizing HMG-CoA Synthase Expression for Enhanced Limonene Production in Escherichia coli through Temporal Transcription Modulation Using Optogenetics.
Article Snippet: Overexpression of a single enzyme in a multigene heterologous pathway may be out of balance with the other enzymes in the pathway, leading to accumulated toxic intermediates, imbalanced carbon flux, reduced productivity of the pathway, or an inhibited growth phenotype.. Therefore, optimal, balanced, and synchronized expression levels of enzymes in a particular metabolic pathway is critical to maximize production of desired compounds while maintaining cell fitness in a growing culture.. Furthermore, the optimal intracellular concentration of an enzyme is determined by the expression strength, specific timing/duration, and degradation rate of the enzyme.

Sequencing:

Article Title: A model-driven approach towards rational microbial bioprocess optimization.
Article Snippet: Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products.. Nevertheless, the nonhomogenous variations of extracellular environment in bioprocesses often challenge the biomass growth and the bioproduction yield.. To enable a more rational bioprocess optimization, we have established a model-driven approach which systematically integrates experiments with modelling, executed from flask to bioreactor scale, using ferulic acid to vanillin bioconversion as a case study.

Article Title: Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae.
Article Snippet: New strategies to control cholera are urgently needed.. This study develops an in vitro proof-ofconcept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach.. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein (YebF-Art-085), thereby self-lysing to release the killing protein (Art-085) to kill V. cholerae.

Article Title: Cell-Free Optogenetic Gene Expression System.
Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Optimizing HMG-CoA Synthase Expression for Enhanced Limonene Production in Escherichia coli through Temporal Transcription Modulation Using Optogenetics.
Article Snippet: Overexpression of a single enzyme in a multigene heterologous pathway may be out of balance with the other enzymes in the pathway, leading to accumulated toxic intermediates, imbalanced carbon flux, reduced productivity of the pathway, or an inhibited growth phenotype.. Therefore, optimal, balanced, and synchronized expression levels of enzymes in a particular metabolic pathway is critical to maximize production of desired compounds while maintaining cell fitness in a growing culture.. Furthermore, the optimal intracellular concentration of an enzyme is determined by the expression strength, specific timing/duration, and degradation rate of the enzyme.



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Image Search Results


Targets for next generation  sequencing  assay, chromosomal location, length of the amplified regions and number of amplicons per target.

Journal: Oncology Letters

Article Title: Determination of EGFR and KRAS mutational status in Greek non-small-cell lung cancer patients

doi: 10.3892/ol.2015.3600

Figure Lengend Snippet: Targets for next generation sequencing assay, chromosomal location, length of the amplified regions and number of amplicons per target.

Article Snippet: A pool of custom upstream and downstream primers were designed using the web-based sequencing assay design tool Design Studio (Illumina, Inc.) ( http:designstudio.illumina.com/ ).

Techniques: Next-Generation Sequencing, Amplification

Comparison of Sanger  sequencing,  HRM and NGS methods used for mutation detection.

Journal: Oncology Letters

Article Title: Determination of EGFR and KRAS mutational status in Greek non-small-cell lung cancer patients

doi: 10.3892/ol.2015.3600

Figure Lengend Snippet: Comparison of Sanger sequencing, HRM and NGS methods used for mutation detection.

Article Snippet: A pool of custom upstream and downstream primers were designed using the web-based sequencing assay design tool Design Studio (Illumina, Inc.) ( http:designstudio.illumina.com/ ).

Techniques: Comparison, Sequencing, Mutagenesis

Difference graph, melting curves and sequencing chromatograms of the sensitivity test for the A746-E750del mutation in exon 19 of the epidermal growth factor receptor gene. Serial dilutions were performed in order to obtain a mutant to wild-type allele ratio of 12.5, 7.5 and 5%.

Journal: Oncology Letters

Article Title: Determination of EGFR and KRAS mutational status in Greek non-small-cell lung cancer patients

doi: 10.3892/ol.2015.3600

Figure Lengend Snippet: Difference graph, melting curves and sequencing chromatograms of the sensitivity test for the A746-E750del mutation in exon 19 of the epidermal growth factor receptor gene. Serial dilutions were performed in order to obtain a mutant to wild-type allele ratio of 12.5, 7.5 and 5%.

Article Snippet: A pool of custom upstream and downstream primers were designed using the web-based sequencing assay design tool Design Studio (Illumina, Inc.) ( http:designstudio.illumina.com/ ).

Techniques: Sequencing, Mutagenesis