web-based sequence designer (Benchling Inc)
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Web Based Sequence Designer, supplied by Benchling Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/web-based+sequence+designer/web+based+sequence+designer/pm39498890-240-12-14
Average 90 stars, based on 1 article reviews
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Plasmid Preparation:Article Title: A model-driven approach towards rational microbial bioprocess optimization. Article Snippet: Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products.. Nevertheless, the nonhomogenous variations of extracellular environment in bioprocesses often challenge the biomass growth and the bioproduction yield.. To enable a more rational bioprocess optimization, we have established a model-driven approach which systematically integrates experiments with modelling, executed from flask to bioreactor scale, using ferulic acid to vanillin bioconversion as a case study. Article Title: Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae. Article Snippet: New strategies to control cholera are urgently needed.. This study develops an in vitro proof-ofconcept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach.. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein (YebF-Art-085), thereby self-lysing to release the killing protein (Art-085) to kill V. cholerae. Article Title: Cell-Free Optogenetic Gene Expression System. Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society. Article Title: Optimizing HMG-CoA Synthase Expression for Enhanced Limonene Production in Escherichia coli through Temporal Transcription Modulation Using Optogenetics. Article Snippet: Overexpression of a single enzyme in a multigene heterologous pathway may be out of balance with the other enzymes in the pathway, leading to accumulated toxic intermediates, imbalanced carbon flux, reduced productivity of the pathway, or an inhibited growth phenotype.. Therefore, optimal, balanced, and synchronized expression levels of enzymes in a particular metabolic pathway is critical to maximize production of desired compounds while maintaining cell fitness in a growing culture.. Furthermore, the optimal intracellular concentration of an enzyme is determined by the expression strength, specific timing/duration, and degradation rate of the enzyme. Sequencing:Article Title: A model-driven approach towards rational microbial bioprocess optimization. Article Snippet: Due to sustainability concerns, bio-based production capitalizing on microbes as cell factories is in demand to synthesize valuable products.. Nevertheless, the nonhomogenous variations of extracellular environment in bioprocesses often challenge the biomass growth and the bioproduction yield.. To enable a more rational bioprocess optimization, we have established a model-driven approach which systematically integrates experiments with modelling, executed from flask to bioreactor scale, using ferulic acid to vanillin bioconversion as a case study. Article Title: Repurposing a Two-Component System-Based Biosensor for the Killing of Vibrio cholerae. Article Snippet: New strategies to control cholera are urgently needed.. This study develops an in vitro proof-ofconcept sense-and-kill system in a wild-type Escherichia coli strain to target the causative pathogen Vibrio cholerae using a synthetic biology approach.. Our engineered E. coli specifically detects V. cholerae via its quorum-sensing molecule CAI-1 and responds by expressing the lysis protein (YebF-Art-085), thereby self-lysing to release the killing protein (Art-085) to kill V. cholerae. Article Title: Cell-Free Optogenetic Gene Expression System. Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society. Article Title: Optimizing HMG-CoA Synthase Expression for Enhanced Limonene Production in Escherichia coli through Temporal Transcription Modulation Using Optogenetics. Article Snippet: Overexpression of a single enzyme in a multigene heterologous pathway may be out of balance with the other enzymes in the pathway, leading to accumulated toxic intermediates, imbalanced carbon flux, reduced productivity of the pathway, or an inhibited growth phenotype.. Therefore, optimal, balanced, and synchronized expression levels of enzymes in a particular metabolic pathway is critical to maximize production of desired compounds while maintaining cell fitness in a growing culture.. Furthermore, the optimal intracellular concentration of an enzyme is determined by the expression strength, specific timing/duration, and degradation rate of the enzyme. |
