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Nude or BALB/c mice received administration of a variety of NIR fluorescence imaging agents to help to define specific organ regions and the appropriate placement of regions of interest, ROI, for quantification. A , GastroSense 680 was orally gavaged, and mice were imaged immediately to visualize the stomach and to place a 3-dimensional ROI (see front and side views). At 2 h post-gavage, the agent emptied from the stomach and moved into the intestines, allowing clear 3D intestinal ROI placement. B , Free fluorophore (VivoTag <t>680XL)</t> was injected IV, revealing very rapid appearance in the bladder (left panels) and circulation through the vessels of the brain (right panels). C , BSA, known as a protein that predominantly localizes to the liver, was labeled with a NIR fluorophore (VivoTag 680XL) and injected IV to define the liver region. D , The large NIR vascular agent, AngioSense 680, was injected IV to provide signal defining the heart and carotid/jugular veins. E , Asthma-like inflammation was induced by immunization of BALB/c mice as previously described , and ProSense 680 was used to image the pulmonary eosinophilia cathepsin activity, thus defining the lung regions in 3D. F , IV ReninSense 680 was activated by normal kidney renin activity, providing signal defining the kidney regions. The resulting 3D ROIs for each of these organ/tissue regions defined the regions to be analyzed in subsequent whole body biodistribution studies, with a 2X4 grid providing a reference for proper ROI placement. Fluorescence outside of each tissue region’s ROI is omitted for clarity.
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Nude or BALB/c mice received administration of a variety of NIR fluorescence imaging agents to help to define specific organ regions and the appropriate placement of regions of interest, ROI, for quantification. A , GastroSense 680 was orally gavaged, and mice were imaged immediately to visualize the stomach and to place a 3-dimensional ROI (see front and side views). At 2 h post-gavage, the agent emptied from the stomach and moved into the intestines, allowing clear 3D intestinal ROI placement. B , Free fluorophore (VivoTag <t>680XL)</t> was injected IV, revealing very rapid appearance in the bladder (left panels) and circulation through the vessels of the brain (right panels). C , BSA, known as a protein that predominantly localizes to the liver, was labeled with a NIR fluorophore (VivoTag 680XL) and injected IV to define the liver region. D , The large NIR vascular agent, AngioSense 680, was injected IV to provide signal defining the heart and carotid/jugular veins. E , Asthma-like inflammation was induced by immunization of BALB/c mice as previously described , and ProSense 680 was used to image the pulmonary eosinophilia cathepsin activity, thus defining the lung regions in 3D. F , IV ReninSense 680 was activated by normal kidney renin activity, providing signal defining the kidney regions. The resulting 3D ROIs for each of these organ/tissue regions defined the regions to be analyzed in subsequent whole body biodistribution studies, with a 2X4 grid providing a reference for proper ROI placement. Fluorescence outside of each tissue region’s ROI is omitted for clarity.
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near-infrared dye vivotag 680  (VisEn Medical)
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Nude or BALB/c mice received administration of a variety of NIR fluorescence imaging agents to help to define specific organ regions and the appropriate placement of regions of interest, ROI, for quantification. A , GastroSense 680 was orally gavaged, and mice were imaged immediately to visualize the stomach and to place a 3-dimensional ROI (see front and side views). At 2 h post-gavage, the agent emptied from the stomach and moved into the intestines, allowing clear 3D intestinal ROI placement. B , Free fluorophore (VivoTag <t>680XL)</t> was injected IV, revealing very rapid appearance in the bladder (left panels) and circulation through the vessels of the brain (right panels). C , BSA, known as a protein that predominantly localizes to the liver, was labeled with a NIR fluorophore (VivoTag 680XL) and injected IV to define the liver region. D , The large NIR vascular agent, AngioSense 680, was injected IV to provide signal defining the heart and carotid/jugular veins. E , Asthma-like inflammation was induced by immunization of BALB/c mice as previously described , and ProSense 680 was used to image the pulmonary eosinophilia cathepsin activity, thus defining the lung regions in 3D. F , IV ReninSense 680 was activated by normal kidney renin activity, providing signal defining the kidney regions. The resulting 3D ROIs for each of these organ/tissue regions defined the regions to be analyzed in subsequent whole body biodistribution studies, with a 2X4 grid providing a reference for proper ROI placement. Fluorescence outside of each tissue region’s ROI is omitted for clarity.
Near Infrared Dye Vivotag 680, supplied by VisEn Medical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nude or BALB/c mice received administration of a variety of NIR fluorescence imaging agents to help to define specific organ regions and the appropriate placement of regions of interest, ROI, for quantification. A , GastroSense 680 was orally gavaged, and mice were imaged immediately to visualize the stomach and to place a 3-dimensional ROI (see front and side views). At 2 h post-gavage, the agent emptied from the stomach and moved into the intestines, allowing clear 3D intestinal ROI placement. B , Free fluorophore (VivoTag 680XL) was injected IV, revealing very rapid appearance in the bladder (left panels) and circulation through the vessels of the brain (right panels). C , BSA, known as a protein that predominantly localizes to the liver, was labeled with a NIR fluorophore (VivoTag 680XL) and injected IV to define the liver region. D , The large NIR vascular agent, AngioSense 680, was injected IV to provide signal defining the heart and carotid/jugular veins. E , Asthma-like inflammation was induced by immunization of BALB/c mice as previously described , and ProSense 680 was used to image the pulmonary eosinophilia cathepsin activity, thus defining the lung regions in 3D. F , IV ReninSense 680 was activated by normal kidney renin activity, providing signal defining the kidney regions. The resulting 3D ROIs for each of these organ/tissue regions defined the regions to be analyzed in subsequent whole body biodistribution studies, with a 2X4 grid providing a reference for proper ROI placement. Fluorescence outside of each tissue region’s ROI is omitted for clarity.

Journal: PLoS ONE

Article Title: Quantitative Whole Body Biodistribution of Fluorescent-Labeled Agents by Non-Invasive Tomographic Imaging

doi: 10.1371/journal.pone.0020594

Figure Lengend Snippet: Nude or BALB/c mice received administration of a variety of NIR fluorescence imaging agents to help to define specific organ regions and the appropriate placement of regions of interest, ROI, for quantification. A , GastroSense 680 was orally gavaged, and mice were imaged immediately to visualize the stomach and to place a 3-dimensional ROI (see front and side views). At 2 h post-gavage, the agent emptied from the stomach and moved into the intestines, allowing clear 3D intestinal ROI placement. B , Free fluorophore (VivoTag 680XL) was injected IV, revealing very rapid appearance in the bladder (left panels) and circulation through the vessels of the brain (right panels). C , BSA, known as a protein that predominantly localizes to the liver, was labeled with a NIR fluorophore (VivoTag 680XL) and injected IV to define the liver region. D , The large NIR vascular agent, AngioSense 680, was injected IV to provide signal defining the heart and carotid/jugular veins. E , Asthma-like inflammation was induced by immunization of BALB/c mice as previously described , and ProSense 680 was used to image the pulmonary eosinophilia cathepsin activity, thus defining the lung regions in 3D. F , IV ReninSense 680 was activated by normal kidney renin activity, providing signal defining the kidney regions. The resulting 3D ROIs for each of these organ/tissue regions defined the regions to be analyzed in subsequent whole body biodistribution studies, with a 2X4 grid providing a reference for proper ROI placement. Fluorescence outside of each tissue region’s ROI is omitted for clarity.

Article Snippet: N-hydroxysuccinimide (NHS)-ester labeling of proteins was performed using the 680 nm fluorophore (VivoTag 680XL, PerkinElmer).

Techniques: Fluorescence, Imaging, Injection, Labeling, Activity Assay

Characteristics of Fluorescent-Labeled Agents.

Journal: PLoS ONE

Article Title: Quantitative Whole Body Biodistribution of Fluorescent-Labeled Agents by Non-Invasive Tomographic Imaging

doi: 10.1371/journal.pone.0020594

Figure Lengend Snippet: Characteristics of Fluorescent-Labeled Agents.

Article Snippet: N-hydroxysuccinimide (NHS)-ester labeling of proteins was performed using the 680 nm fluorophore (VivoTag 680XL, PerkinElmer).

Techniques: Labeling