Journal: BMC Plant Biology

Article Title: Comparative plastid genome analysis of four different variants of Gastrodia elata and development of molecular markers based on plastid DNA sequences

doi: 10.1186/s12870-026-08695-4

Figure Lengend Snippet: Frontal view of tubers of different G. elata variants from various localities. A. G. elata f. glauca ; B. G. elata f. elata ; C. G. elata f. viridis ; D. G. elata f. flavida ; E. G. elata f. elata ‘XueHong’ (a cultivar of G. elata f. elata ). Abbreviations: YN: Collected from Yunnan Province; GZ: Collected from Guizhou Province; HB: Collected from Hubei Province; SX: Collected from Shaanxi Province; CQ: Collected from Chongqing Municipality; SC: Collected from Sichuan Province. Numbered codes represent biological replicates. Tuber morphology: Elliptical: CQ1, GZ6, GZ10, HB9, SC1, YN1, YN2. Long-Elliptical: HB5, YN6, YN11, YN12, YN16. Oval-Elliptical: GZ1, GZ7, GZ8, GZ9. Obovate-Elliptical: CQ2, CQ3, GZ2, GZ5, HB1, HB2, HB8, HB10, YN5, YN9, YN10, YN13, YN15. Obconical: GZ3, SC3, SX2, YN3, YN8. Nearly Cylindrical: CQ4, HB3, HB4, SC2, SX1, SX4. Cylindrical: SC4, YN14. Long-Cylindrical: GZ4, SX3. Dumbbell-Shaped: YN4, YN7. Fusiform: HB6, HB7

Article Snippet: SC-v1 , G. elata f. viridis , Pingwu, Mianyang, Sichuan , 104°52′E; 32°17′N , For WGS.

Techniques:

Journal: BMC Plant Biology

Article Title: Comparative plastid genome analysis of four different variants of Gastrodia elata and development of molecular markers based on plastid DNA sequences

doi: 10.1186/s12870-026-08695-4

Figure Lengend Snippet: Plant habit, Flower (showing labellum), and capsule of four G. elata variants. A G. elata f. elata (Sichuan type); B G. elata f. elata (Yunnan type); C G. elata f. viridis (Sichuan type); D G. elata f. viridis (Yunnan type); E G. elata f. glauca ; F G. elata f. flavida . 1: Raceme; 2: Flower and its longitudinal section; 3: Labellum, side view and front view; 4: Capsule. The red arrow indicates the perianth tube and its apical lobes on a G. elata flower. The yellow arrow points to the inferior ovary and its distinctive color or texture. The green arrow highlights the bracts associated with the flower and the inflorescence. The white arrow shows the fringe at the apex of the floral labellum. The gray arrow indicates the calli at the base of the labellum. The blue arrow identifies a mature capsule and its specific color or texture

Article Snippet: SC-v1 , G. elata f. viridis , Pingwu, Mianyang, Sichuan , 104°52′E; 32°17′N , For WGS.

Techniques:

Journal: BMC Plant Biology

Article Title: Comparative plastid genome analysis of four different variants of Gastrodia elata and development of molecular markers based on plastid DNA sequences

doi: 10.1186/s12870-026-08695-4

Figure Lengend Snippet: The circular map of the plastome of four G. elata variants. Genomic features on transcriptionally clockwise and counter-clockwise strands are drawn on the inside and outside of the circle, respectively. Genes belonging to different functional groups are color-coded

Article Snippet: SC-v1 , G. elata f. viridis , Pingwu, Mianyang, Sichuan , 104°52′E; 32°17′N , For WGS.

Techniques: Functional Assay

Journal: BMC Plant Biology

Article Title: Comparative plastid genome analysis of four different variants of Gastrodia elata and development of molecular markers based on plastid DNA sequences

doi: 10.1186/s12870-026-08695-4

Figure Lengend Snippet: Sequence similarity comparison among G. elata plastomes. A Nucleotide diversity (Pi) of 11 G. elata plastomes. Most regions of these 11 plastomes show a Pi value of 0, indicating extremely high sequence conservation. Only a few regions exhibit sequence variations, with the maximum Pi value of 0.01018 observed in the intervals 7117–7291 and 30,738–30,912. B Sequence similarity among the 11 plastomes compared using mVISTA, with annotations from PX233521 used as the reference. Annotated gene information is displayed at the top of the window. Arrows indicate gene orientation, dark blue backgrounds represent protein-coding genes, light blue backgrounds represent RNA-coding genes, and red areas represent non-coding regions. Genomic positions (in base pairs, bp) are labeled below the window

Article Snippet: SC-v1 , G. elata f. viridis , Pingwu, Mianyang, Sichuan , 104°52′E; 32°17′N , For WGS.

Techniques: Sequencing, Comparison, Labeling

Journal: BMC Plant Biology

Article Title: Comparative plastid genome analysis of four different variants of Gastrodia elata and development of molecular markers based on plastid DNA sequences

doi: 10.1186/s12870-026-08695-4

Figure Lengend Snippet: The phylogenetic relationships of the four variants of G. elata . This tree was constructed based on the nucleotide sequences of the whole plastomes of 13 samples. Among which, G. angusta ( NC_066060.1 ) and G. javanica ( NC_066063.1 ) were used as outgroups. The ML and BI tree having the same topological structure. The tree on the top left corner displays the original branch lengths, while the tree on the right side depicts the tree with branch lengths ignored. The tree is indicated with ML bootstrap support values and BI posterior probabilities at each node

Article Snippet: SC-v1 , G. elata f. viridis , Pingwu, Mianyang, Sichuan , 104°52′E; 32°17′N , For WGS.

Techniques: Construct

Journal: Scientific Reports

Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

doi: 10.1038/s41598-025-11509-6

Figure Lengend Snippet: Survival of different bacterial strains upon disinfection with steam. The different plots present the colony-forming units (CFUs) of various bacterial strains before and after steam treatment for 10, 30–60 s and subsequent plating on LB agar: ( a ). S. aureus ATCC15981 (MSSA); ( b ). S. aureus E75 (MSSA); ( c ). S. aureus E166 (MSSA); ( d ). S. aureus E276 (MSSA); ( e ). S. aureus USA300 (MRSA); ( f ). S. aureus HG001 (MSSA); ( g ). S. epidermidis ATCC35984; h. E. coli ATCC25922; i. P. aeruginosa ATCC27853; j. K. oxytoca ; k. K. pneumoniae ATCC15883; and l. A. baumannii ATCC19606. The residual CFU counts are indicated as a percentage of the CFU counts of the inoculum on top of each bar. All experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

Techniques: Serial Time-encoded Amplified Microscopy

Journal: Scientific Reports

Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

doi: 10.1038/s41598-025-11509-6

Figure Lengend Snippet: Disinfection of nebulizer parts with steam. The different parts of a PARI LC SPRINT Nebulizer handset were contaminated with: ( a ) S. aureus ATCC15981; ( b ) S. aureus E75; (c) S. aureus E166; ( d ) S. aureus E276; ( e ) S. aureus USA300; ( f ) S. aureus HG001; ( g ) S. epidermidis ATCC35984; ( h ) E. coli ATCC25922; ( i ) P. aeruginosa ATCC27853; ( j ) K. oxytoca ; ( k ) K. pneumoniae ATCC15883; ( l ) A. baumanni ATCC19606. Prior and after 60 s steam exposure, the contaminated mouth piece was replica-plated on LB agar. Bacteria contaminating the connection tube and nozzle were collected by swabbing and subsequently plated on LB agar. Of note, the different bacterial loads on the contaminated nebulizer parts were not quantified. Upper left plate of each panel, replica-plated contaminated mouthpiece; lower left plate, replica-plated contaminated mouthpiece after disinfection with steam; upper right part plate, bacteria contaminating the connection tube and nozzle; lower right plate, bacteria contaminating the connection tube and nozzle after steam disinfection. Three independent replicate experiments were performed ( n = 3).

Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

Techniques: Serial Time-encoded Amplified Microscopy, Bacteria

Journal: Scientific Reports

Article Title: Disinfection of medical devices with a steam machine that operates at atmospheric pressure and is suitable for home usage

doi: 10.1038/s41598-025-11509-6

Figure Lengend Snippet: Effects of steam exposure on bacterial biofilms. Biofilms of different bacterial species were allowed to form in 96-well micro liter plates during 24 h of growth. Subsequently, the biofilms were exposed to steam for 1, 5–10 min. The biofilms and a non-sterilized control plate were then stained with crystal violet ( a ). In addition, the crystal violet retained by the biofilms was dissolved in ethanol and the released crystal violet was quantified spectrophotometrically ( b ). The bacterial strains used were S. aureus ATCC15981, S. aureus E75, S. aureus E166, S. aureus E276, S. aureus USA300, S. aureus HG001, S. epidermidis ATCC35984, E. coli ATCC25922, P. aeruginosa ATCC27853, K. oxytoca , K. pneumoniae ATCC15883 and A. baumannii ATCC19606. Experiments were performed in triplicate ( n = 3). Standard deviations in the CFU counts are indicated by error bars. *, P < 0.05.

Article Snippet: P. aeruginosa ATCC , 2.8 ± 0.58*10 8 , 13.3 ± 2.7*10 3 , 0 , 0.

Techniques: Serial Time-encoded Amplified Microscopy, Control, Staining