Journal: bioRxiv

Article Title: Spore-Based Biocomposite Thermoplastic Polyesters with Enhanced Toughness and Programmable Disintegration

doi: 10.64898/2026.02.24.707801

Figure Lengend Snippet: Schematic illustration of the fabrication of biocomposite polyesters filled with bacterial spores. Heat shock tolerized (HST) B. subtilis spores (ATCC 6633 HST strain) are used as biofillers for PLA, PCL and PBAT polyesters.

Article Snippet: Optimized processing temperatures of the selected PCL, PLA and PBAT materials in a twin screw, corotating microcompounder were 65 °C, 120 °C and 135 °C, respectively, all of which are below the temperature threshold for high ATCC 6633 HST spore viability.

Techniques:

Journal: Journal of Cellular and Molecular Medicine

Article Title: The Role of HER2 and RANK in Breast Cancer and New Therapeutic Approaches With Denosumab, Anti‐HER2 Antibodies and Immunotherapy

doi: 10.1111/jcmm.71071

Figure Lengend Snippet: Cell viability levels of SK‐BR‐3 and MCF‐7 determined by MTT assay. (A, B) Effects of single (A) and in combination (B) therapeutic agents on cell viability in HER2 + (SK‐BR‐3) and HER2 − (MCF‐7) breast cancer (BC) cell lines. The data is expressed as mean ± SD of three independent experiments ( N = 3). Statistical significance was determined by ANOVA followed by Dunnett's T3 post hoc test, where * p ‐value < 0.05; ** p ‐value < 0.01; *** p ‐value < 0.001 in comparison with the control group. (C) Experiments at the level of 3D cell lines, when incubated with D+T+P+N+I (Denosumab+Trastuzumab+Pertuzumab+Nivolumab+Ipilimumab) in HER2 + BC, resulted in a clinically significant reduction in cell viability, possibly through immunomodulation of the tumour microenvironment (TME), characterised by a marked increase in tumour‐infiltrating lymphocytes (TILs) infiltration and a possibly concurrent decrease in pro‐tumorigenic neutrophils (created using BioRender ( https://biorender.com/ )). ANOVA, analysis of variance; MTT, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide; SD, standard deviation.

Article Snippet: Following treatment, the reagent 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) (30006, Biotium Inc., Landing Parkway, Fremont, CA, USA) was added to each well at a final concentration of 0.5 mg/mL.

Techniques: MTT Assay, Comparison, Control, Incubation, Standard Deviation