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Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro <t>vertical</t> <t>diffusion</t> chamber <t>(VDC)</t> infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
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Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro <t>vertical</t> <t>diffusion</t> chamber <t>(VDC)</t> infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
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Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro <t>vertical</t> <t>diffusion</t> chamber <t>(VDC)</t> infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.
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The most commonly used in vitro diffusion investigation devices.
Navicyte Vertical Diffusion Chamber System, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
navicyte vertical diffusion chamber system - by Bioz Stars, 2026-07
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Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

Journal: Journal of Bacteriology

Article Title: Regulatory Role of Anti-Sigma Factor RsbW in Clostridioides difficile Stress Response, Persistence, and Infection

doi: 10.1128/jb.00466-22

Figure Lengend Snippet: Δ rsbW demonstrates increased cell adhesion but decreased virulence. (A) WT C. difficile , Δ rsbW , and Δ rsbW + rsbW adherence to epithelial cells across 3, 6, 12, and 24 h after infection in an in vitro vertical diffusion chamber (VDC) infection model ( N = 3, with 3 technical replicates/experiment). (B) Survival curve of Galleria mellonella infected with C. difficile WT, Δ rsbW , and Δ rsbW + rsbW strains across 24 and 72 h (N = 5, with 8 larvae/strain/time point). (C) Adherent bacterial population recovered from infected G. mellonella gut enumerated with CFU/mL on brain-heart infusion with 0.5% (wt/vol) yeast extract and 0.1% (wt/vol) l -cysteine (BHI-S) agar. Error bars indicate SD. n.s., no significance; *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001, as determined by Mann-Whitney U test and log rank Mantel Cox test for the survival curve.

Article Snippet: The Snapwell inserts, containing polarized Caco-2, HT29, and CCD 18co myofibroblasts, were each sandwiched between two compartments of the vertical diffusion chamber (VDC) (Harvard Apparatus, UK).

Techniques: Infection, In Vitro, Diffusion-based Assay, MANN-WHITNEY

Comparison of different diffusion cell types.

Journal: Pharmaceutics

Article Title: Skin-on-a-Chip Technology for Testing Transdermal Drug Delivery—Starting Points and Recent Developments

doi: 10.3390/pharmaceutics13111852

Figure Lengend Snippet: Comparison of different diffusion cell types.

Article Snippet: Navicyte Vertical Diffusion Chamber System , Warner Instruments, Holliston, MA, USA , Static, vertical , Characterization of the intestinal, corneal, or nasal permeability , [ , , ] .

Techniques: Comparison, Diffusion-based Assay, Permeability, Dissolution, Ex Vivo

The most commonly used in vitro diffusion investigation devices.

Journal: Pharmaceutics

Article Title: The Development of an In Vitro Horizontal Diffusion Cell to Monitor Nasal Powder Penetration Inline

doi: 10.3390/pharmaceutics13060809

Figure Lengend Snippet: The most commonly used in vitro diffusion investigation devices.

Article Snippet: Navicyte Vertical Diffusion Chamber System (Harvard Apparatus, Holliston, Massachusetts, United States) , Cells, ex vivo tissue investigation Intestinal and nasal permeability , Vertical , Both phases: variable Real-time analysis is possible.

Techniques: In Vitro, Diffusion-based Assay, Ex Vivo, Permeability