Journal: bioRxiv

Article Title: Whole genome sequencing of three mesorhizobia isolated from northern Canada to identify genomic adaptations promoting nodulation in cold climates

doi: 10.1101/2022.04.26.489235

Figure Lengend Snippet: Cell density (OD 600 ) of Mesorhizobium cultures following ∼ 7 days (10°C), ∼ 2 days (15°C), or ∼ 1 day (20°C to 32.5°C) of growth at the indicated temperature. Starting OD 600 values were ∼ 0.05 for all cultures. Three biological replicates were performed, with each symbol representing a single replicate. Blue squares – M. sp. AR02; yellow diamonds – M. sp. AR07; red circles – M. sp. AR10; grey triangles – M. japonicum MAFF303099.

Article Snippet: The RefSeq version of all 94 Mesorhizobium genomes that were either classified as a RefSeq representative genome or that had an assembly level of ‘complete’ or ‘chromosome’ were downloaded from the National Center for Biotechnology Information (NCBI) Genome database.

Techniques:

Journal: bioRxiv

Article Title: Whole genome sequencing of three mesorhizobia isolated from northern Canada to identify genomic adaptations promoting nodulation in cold climates

doi: 10.1101/2022.04.26.489235

Figure Lengend Snippet: A maximum likelihood phylogeny of the NodC proteins of the 97 Mesorhizobium strains shown in . Nodes with a bootstrap value of ≥ 90 are indicated with gray dots, based on 352 bootstrap replicates. The scale represents the mean number of amino acid substitutions per site. Strains isolated and sequenced in this study are shown in blue, boldface font. M. loti 582 and M. huakuii 583, also isolated from Oxytropis plants in a subarctic climatic region, are shown in red, boldface font.

Article Snippet: The RefSeq version of all 94 Mesorhizobium genomes that were either classified as a RefSeq representative genome or that had an assembly level of ‘complete’ or ‘chromosome’ were downloaded from the National Center for Biotechnology Information (NCBI) Genome database.

Techniques: Isolation