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Effects of TTX analogues on the viability of mouse neuroblastoma cells, Neuro-2a, exposed to ouabain and <t>veratridine.</t> Cell viability vs TTXs concentration (data points are means ± SD) and estimated EC 50 values (means ± SD, n = 4). Symbols indicate (●) TTX ( 1 ), (■) 6- epi -11-deoxyTTX ( 2 ).
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Effects of TTX analogues on the viability of mouse neuroblastoma cells, Neuro-2a, exposed to ouabain and <t>veratridine.</t> Cell viability vs TTXs concentration (data points are means ± SD) and estimated EC 50 values (means ± SD, n = 4). Symbols indicate (●) TTX ( 1 ), (■) 6- epi -11-deoxyTTX ( 2 ).
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Effects of TTX analogues on the viability of mouse neuroblastoma cells, Neuro-2a, exposed to ouabain and <t>veratridine.</t> Cell viability vs TTXs concentration (data points are means ± SD) and estimated EC 50 values (means ± SD, n = 4). Symbols indicate (●) TTX ( 1 ), (■) 6- epi -11-deoxyTTX ( 2 ).
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Effects of TTX analogues on the viability of mouse neuroblastoma cells, Neuro-2a, exposed to ouabain and veratridine. Cell viability vs TTXs concentration (data points are means ± SD) and estimated EC 50 values (means ± SD, n = 4). Symbols indicate (●) TTX ( 1 ), (■) 6- epi -11-deoxyTTX ( 2 ).

Journal: Journal of Natural Products

Article Title: Identification of Deoxy- and epi -Tetrodotoxin Analogues from the Newt Cynops ensicauda popei Suggests Stepwise Oxidation in Terrestrial Tetrodotoxin Biosynthesis

doi: 10.1021/acs.jnatprod.5c01546

Figure Lengend Snippet: Effects of TTX analogues on the viability of mouse neuroblastoma cells, Neuro-2a, exposed to ouabain and veratridine. Cell viability vs TTXs concentration (data points are means ± SD) and estimated EC 50 values (means ± SD, n = 4). Symbols indicate (●) TTX ( 1 ), (■) 6- epi -11-deoxyTTX ( 2 ).

Article Snippet: Cells (100 μL/well) were precultured in 96-well plates (TPP) under 5% CO 2 at 37 °C for 11 h. Test medium (100 μL) containing TTX or 2 in various concentrations together with ouabain (200 μM, Sigma-Aldrich), and veratridine (110 μM, Alomone Laboratories) was then added.

Techniques: Analogues, Concentration Assay