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vaccinia ce vce  (New England Biolabs)
96
New England Biolabs vaccinia ce vce
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Vaccinia Ce Vce, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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vaccinia ce vce - by Bioz Stars, 2026-04
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vce [cluster centre] option  (STATA Corporation)
90
STATA Corporation vce [cluster centre] option
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Vce [Cluster Centre] Option, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vce [cluster centre] option/product/STATA Corporation
Average 90 stars, based on 1 article reviews
vce [cluster centre] option - by Bioz Stars, 2026-04
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vce(hc3) command  (STATA Corporation)
90
STATA Corporation vce(hc3) command
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Vce(Hc3) Command, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vce(hc3) command/product/STATA Corporation
Average 90 stars, based on 1 article reviews
vce(hc3) command - by Bioz Stars, 2026-04
90/100 stars
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vce  (New England Biolabs)
96
New England Biolabs vce
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Vce, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vce/product/New England Biolabs
Average 96 stars, based on 1 article reviews
vce - by Bioz Stars, 2026-04
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rt-vce mc1200  (IntroMedic Co Ltd)
90
IntroMedic Co Ltd rt-vce mc1200
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Rt Vce Mc1200, supplied by IntroMedic Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-vce mc1200/product/IntroMedic Co Ltd
Average 90 stars, based on 1 article reviews
rt-vce mc1200 - by Bioz Stars, 2026-04
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rt-vce mirocam  (IntroMedic Co Ltd)
90
IntroMedic Co Ltd rt-vce mirocam
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Rt Vce Mirocam, supplied by IntroMedic Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-vce mirocam/product/IntroMedic Co Ltd
Average 90 stars, based on 1 article reviews
rt-vce mirocam - by Bioz Stars, 2026-04
90/100 stars
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rt-vce system mirocam mc1200  (IntroMedic Co Ltd)
90
IntroMedic Co Ltd rt-vce system mirocam mc1200
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Rt Vce System Mirocam Mc1200, supplied by IntroMedic Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-vce system mirocam mc1200/product/IntroMedic Co Ltd
Average 90 stars, based on 1 article reviews
rt-vce system mirocam mc1200 - by Bioz Stars, 2026-04
90/100 stars
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vce cluster option  (STATA Corporation)
90
STATA Corporation vce cluster option
( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two <t>CEs</t> <t>(MRV_5</t> and MRV_6) found in this work compared with commercial vaccinia CE <t>(VCE)</t> (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.
Vce Cluster Option, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/vce cluster option/product/STATA Corporation
Average 90 stars, based on 1 article reviews
vce cluster option - by Bioz Stars, 2026-04
90/100 stars
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( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two CEs (MRV_5 and MRV_6) found in this work compared with commercial vaccinia CE (VCE) (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.

Journal: Science Advances

Article Title: Discovery of diverse and high-quality mRNA capping enzymes through a language model–enabled platform

doi: 10.1126/sciadv.adt0402

Figure Lengend Snippet: ( A ) Diagram of the in vivo capping activity assay. The construction details of the K1.5/T7 transcriptional system used for the in vivo assays are shown in fig. S15. YFP, yellow fluorescent protein. ( B ) The capping activities of 46 text-mined CEs in CHO cells. The RNAP not fused to a CE served as the negative control (NO_CE, blue bar), and the RNAP fused to the NP868R CE served as the positive control (gray bar and blue dashed line). The relative activities were calculated by assigning the NO_CE mean fluorescence intensity as 1. The bar plot shows the means ± SD of three biological replicates. Sequences chosen for yeast evaluation are marked by orange dots. Data are found in table S5. ( C ) The capping activities of 18 text-mined CEs in S. cerevisiae . Controls, error bars, and color coding are the same as in (B). ( D ) Diagram of the in vitro capping activity assay. ( E ) The in vitro capping activities of two CEs (MRV_5 and MRV_6) found in this work compared with commercial vaccinia CE (VCE) (control_NEB) and no enzyme treatment control (control_NoEnzyme). Each group includes three biological replicates, with representative mass spectra shown. The x axis represents the molecular weight of the compounds, with the observed mass displayed above the corresponding peaks (amu, the relative atomic mass unit). The y axis is the count of compounds collected. For compound identities and liquid chromatography–mass spectrometry (LC-MS) results of all experiments, see figs. S22 to S25 and table S6. The bar plot shows the means ± SD of three biological replicates. P values were determined using an unpaired two-sided t test.

Article Snippet: With the same enzyme usage and reaction conditions, both MRV_5 and MRV_6 [Marseilleviridae CEs (MCEs)] exhibited twofold enhanced capping efficiencies to the commercial vaccinia CE (VCE) from New England Biolabs (NEB; and figs. S22 to S25).

Techniques: In Vivo, Activity Assay, Negative Control, Positive Control, Fluorescence, In Vitro, Control, Molecular Weight, Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy