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Figure 1. <t>MALDI</t> <t>Biotyper-cluster</t> analysis showing a dendrogram of the MSP generated for 20 D. solani strains in contrast to other Dickeya spp. The closeness of the relationships between the studied microorganisms is reflected by arbitrary distance levels normalized to a maximum value of 1000. 449 MALDI-TOF MS spectra of D. solani strains were registered for this analysis (a). Integration of the main protein spectra generated MSP for 20 D. solani strains and the D. dadantii DSM 18020TS MSP, which is the closest match in the Bruker database (log score 1.720), revealed a set of common and unique peaks with the corresponding mass-to-charge (m/z) and relative intensities. Blue peaks derived from the reference D. dadantii MSP are displayed using an inverted intensity scale. The colour of the peaks reflects the degree of matching with the reference MSP; green bars representing a full match, yellow—a partial match, and red—no match (b).
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Figure 1. MALDI Biotyper-cluster analysis showing a dendrogram of the MSP generated for 20 D. solani strains in contrast to other Dickeya spp. The closeness of the relationships between the studied microorganisms is reflected by arbitrary distance levels normalized to a maximum value of 1000. 449 MALDI-TOF MS spectra of D. solani strains were registered for this analysis (a). Integration of the main protein spectra generated MSP for 20 D. solani strains and the D. dadantii DSM 18020TS MSP, which is the closest match in the Bruker database (log score 1.720), revealed a set of common and unique peaks with the corresponding mass-to-charge (m/z) and relative intensities. Blue peaks derived from the reference D. dadantii MSP are displayed using an inverted intensity scale. The colour of the peaks reflects the degree of matching with the reference MSP; green bars representing a full match, yellow—a partial match, and red—no match (b).

Journal: Scientific reports

Article Title: Phyloproteomic study by MALDI-TOF MS in view of intraspecies variation in a significant homogenous phytopathogen Dickeya solani.

doi: 10.1038/s41598-023-46012-3

Figure Lengend Snippet: Figure 1. MALDI Biotyper-cluster analysis showing a dendrogram of the MSP generated for 20 D. solani strains in contrast to other Dickeya spp. The closeness of the relationships between the studied microorganisms is reflected by arbitrary distance levels normalized to a maximum value of 1000. 449 MALDI-TOF MS spectra of D. solani strains were registered for this analysis (a). Integration of the main protein spectra generated MSP for 20 D. solani strains and the D. dadantii DSM 18020TS MSP, which is the closest match in the Bruker database (log score 1.720), revealed a set of common and unique peaks with the corresponding mass-to-charge (m/z) and relative intensities. Blue peaks derived from the reference D. dadantii MSP are displayed using an inverted intensity scale. The colour of the peaks reflects the degree of matching with the reference MSP; green bars representing a full match, yellow—a partial match, and red—no match (b).

Article Snippet: The classification of all strains was verified by creating the MSP dendrogram using the MALDI Biotyper Compass Explorer software v. 4.1.80 (Bruker Daltonics GmbH, Bremen, Germany).

Techniques: Generated, Derivative Assay

Figure 2. MALDI Biotyper-cluster analysis of 20 D. solani strains visualized as a dendrogram of the MSP that revealed intraspecies variation in the MALDI-TOF MS intact protein profiles among the studied isolates. The closeness of the relationships between the investigated microorganisms is reflected by arbitrary distance levels normalized to a maximum value of 1000 (a). Comparison of the MALDI-TOF MS representative spectra, as selected based on a PCA analysis, of four D. solani strains described in this study. The top panel shows the MALDI-TOF MS spectrum registered for D. solani type strain IPO2222TS (IFB0123); the following representative spectra were acquired for D. solani IFB0699, IFB0240 and IFB0130. The assigned masses are depicted in Da (b).

Journal: Scientific reports

Article Title: Phyloproteomic study by MALDI-TOF MS in view of intraspecies variation in a significant homogenous phytopathogen Dickeya solani.

doi: 10.1038/s41598-023-46012-3

Figure Lengend Snippet: Figure 2. MALDI Biotyper-cluster analysis of 20 D. solani strains visualized as a dendrogram of the MSP that revealed intraspecies variation in the MALDI-TOF MS intact protein profiles among the studied isolates. The closeness of the relationships between the investigated microorganisms is reflected by arbitrary distance levels normalized to a maximum value of 1000 (a). Comparison of the MALDI-TOF MS representative spectra, as selected based on a PCA analysis, of four D. solani strains described in this study. The top panel shows the MALDI-TOF MS spectrum registered for D. solani type strain IPO2222TS (IFB0123); the following representative spectra were acquired for D. solani IFB0699, IFB0240 and IFB0130. The assigned masses are depicted in Da (b).

Article Snippet: The classification of all strains was verified by creating the MSP dendrogram using the MALDI Biotyper Compass Explorer software v. 4.1.80 (Bruker Daltonics GmbH, Bremen, Germany).

Techniques: Comparison