Journal: Journal of Surgical Case Reports

Article Title: Urinary bladder matrix for lower extremity split-thickness skin graft donor site

doi: 10.1093/jscr/rjad529

Figure Lengend Snippet: Intraoperative donor site covered with 7 × 10 cm UBM (Acell Lafayette).

Article Snippet: This case study demonstrates the use of urinary bladder matrix (UBM) (Acell Lafayette, IN), a different type of dermal substitute, in lower extremity STSG donor site reconstruction.

Techniques:

Journal: Journal of immunology and regenerative medicine

Article Title: Matrix bound nanovesicle-associated IL-33 activates a pro-remodeling macrophage phenotype via a non-canonical, ST2-independent pathway

doi: 10.1016/j.regen.2019.01.001

Figure Lengend Snippet: (A, B) Decellularization efficacy. The presence of nuclei in native mouse small intestine tissue and decellularized mouse intestine tissue was assessed by hemotoxylin and eosin (H&E) staining and 4’,6-diamidino-2-phenylindole (DAPI) staining (A). Concentration of double stranded DNA was quantified using the PicoGreen assay; y-axis is log scale; n = 4; p <0.005. Red line denotes established DNA criteria against which decellularization efficacy was verified. Native dsDNA concentration = 7747.34 ± 748; Decellularized dsDNA concentration = 49.87 ± 4.84 (B). (C) Transmission electron microscopy imaging of MBV isolated from decellularized mouse intestine. (D) Graphic representation of the quantitation of 15 cytokines with the highest expression levels in MBV isolated from decellularized wt and il33−/− mouse intestines. Shown are mean ± s.d, n=3 biological replicates. (E) Immunoblot analysis of IL-33 expression levels in MBV isolated from three decellularized wt mouse intestines (IL33+ MBV) or three decellularized il33−/− mouse intestines (IL33- MBV). (F) Immunoblot analysis of IL-33 expression levels in MBV isolated from laboratory produced porcine urinary bladder matrix (UBM), small intestinal submucosa (SIS), dermis, and cardiac ECM; and three commercially available ECM bioscaffold equivalents: ACell® MatriStem™ (porcine UBM), BD® XenMatrix™ (porcine dermis), and Cook Biotech® Biodesign™ (porcine SIS).

Article Snippet: The presence of full-length IL-33 expression in MBV was subsequently observed in ECM surgical meshes commonly used in clinical applications, which included laboratory-produced and commercially available equivalents of urinary bladder matrix (UBM) and ACell® MatriStem™; small intestinal submucosa (SIS) and Cook Biotech® BioDesign™; dermis and BD® XenMatrix™; and cardiac ECM ( ).

Techniques: Staining, Concentration Assay, Picogreen Assay, Transmission Assay, Electron Microscopy, Imaging, Isolation, Quantitation Assay, Expressing, Western Blot, Produced