Journal: Cell death & disease

Article Title: Turning anecdotal irradiation-induced anticancer immune responses into reproducible in situ cancer vaccines via disulfiram/copper-mediated enhanced immunogenic cell death of breast cancer cells.

doi: 10.1038/s41419-024-06644-3

Figure Lengend Snippet: Fig. 1 DSF/Cu was cytotoxic to mouse and human BC cells in vitro. A Mouse 4T1, human MDA-MB-231, and UACC812 BC cells were treated with the indicated concentrations of DSF and CuCl2 (1 μM). After 24 h of exposure, cell viability was determined via MTT assays. The DSF/Cu IC50 of each cell line is indicated. B Death of 4T1 cells treated with DSF/Cu observed via visual microscopy. C, D 4T1 (C) and MDA-MB-231 (D) BC cell lines were treated with DSF/Cu for the indicated times, and the late-stage apoptotic (Annexin V+/7-AAD+) cells were determined using flow cytometry. Data of late apoptosis are presented as the mean ± SEM of three replicates from one representative experiment. ***p < 0.001, **p < 0.01 and *p < 0.05 vs control group. The experiments were performed 3 times.

Article Snippet: Mouse 4T1 and human UACC812 BC cell lines were purchased from the American Type Culture Collection (ATCC) and the human MDA-MB-231 BC cell line was acquired from the Duke Comprehensive Cancer Center Cell Culture Facility.

Techniques: In Vitro, Microscopy, Cytometry, Control

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Associations between SGLT1 protein expression levels and clinicopathologic variables of the breast cancer patient cohort.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Expressing

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Representative staining of SGLT1 in different subtypes of breast cancer (×100, ×400). (a) Negative immunoreactions. Scale bar: 100 μ m. (b) Positive staining of SGLT1 in luminal-like breast cancer. (c) Positive staining of SGLT1 in HER2+ breast cancer. (d) Positive staining of SGLT1 in basal-like breast cancer.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Staining

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Representative western blot of HER2 and SGLT1 expression levels and quantification relative to actin in UACC812 and SKBR3 cells. UACC812 and SKBR3 cells were treated with HER2 inhibitors (HER2 I), Phlorizin, MG132 alone, or in combination for 48 h. Untreated cells were used as a negative control (NC). (a) A representative WB detection result of three independent experiments was shown. (b) The relative levels of HER2 and SGLT1 protein in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Western Blot, Expressing, Negative Control

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: The curve and column results of the ECAR values for UACC812 and SKBR3 cells. (a) The ECAR values of UACC812 and SKBR3 cells treated with 0, 10, 20, 30, 40, 50, and 60 min, respectively. (b) The ECAR values of each group at 40 min in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques:

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Representative western blot of PI3K/Akt/mTOR pathway-related protein expression levels and quantification relative to actin in UACC812 cells. A representative WB detection result of PI3K/Akt/mTOR pathway-related protein expression levels in (a) SGLT1 siRNA and (d) phlorizin treatment. The relative levels of PI3K/Akt/mTOR protein in (b) SGLT1 siRNA and (e) phlorizin treatment. The relative levels of p-PI3K/p-Akt/p-mTOR protein in (c) SGLT1 siRNA and (f) phlorizin treatment. The results shown were representative of at least three independent experiments; ∗∗∗∗ p < 0.0001.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Western Blot, Expressing

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: CCK-8 assays for the effect of SGLT1 expression on cell proliferation in UACC812 and SKBR3 cells. (a) The cell viability of UACC812 and SKBR3 cells relative to negative control groups after 0, 12, 24, 36, and 48 h treatment, respectively. (b) The cell viability of each group relative to negative control groups at 48 h in UACC812 and SKBR3 cells. Statistically significant differences between groups were displayed; ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: CCK-8 Assay, Expressing, Negative Control

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Cox's univariate regression analysis of the patient survival in breast cancer with respect to clinical parameters and cancer biological factors.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques:

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Kaplan-Meier survival analysis according to SGLT1 expression in the whole cohort and HER2+ subtype of breast cancer patients (log-rank test). Association of SGLT1 expression levels with (a) DFS and (c) OS in the whole cohort of breast cancer patients. Association of SGLT1 expression levels with (b) DFS and (d) OS in HER2+ breast cancer patients.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Expressing

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Kaplan-Meier survival analysis according to SGLT1 expression in basal-like, luminal-like, and luminal-HER2+ subtype of breast cancer patients (log-rank test). Association of SGLT1 expression levels with (a) DFS and (d) OS in basal-like breast cancer patients. Association of SGLT1 expression levels with (b) DFS and (e) OS in luminal-like breast cancer patients. Association of SGLT1 expression levels with (c) DFS and (f) OS in LuminalHER2+ breast cancer patients.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: Expressing

Journal: Disease Markers

Article Title: Sodium-Dependent Glucose Transporter 1 (SGLT1) Stabled by HER2 Promotes Breast Cancer Cell Proliferation by Activation of the PI3K/Akt/mTOR Signaling Pathway in HER2+ Breast Cancer

doi: 10.1155/2020/6103542

Figure Lengend Snippet: Cox's multivariate regression analysis of the patient survival in breast cancer with respect to clinical parameters and cancer biological factors.

Article Snippet: The HER2+ breast cancer cell lines UACC812 and SKBR3 were originally purchased from the American Type Culture Collection (Manassas, VA, USA) and authenticated by short tandem repeat analysis.

Techniques: