Journal: Materials Today Bio

Article Title: Microcarriers encapsulating COL1A1 mRNA-loaded nanovesicles for skin photoaging treatment

doi: 10.1016/j.mtbio.2026.103126

Figure Lengend Snippet: Scheme of the fabrication of the microcarriers and delivery of engineered nanovesicles for photoaging therapy. Abbreviations: HDF, human dermal fibroblast; Exos, exosomes; Emvs, engineered nanovesicles; HDF COL1A1 Emvs, Emvs derived from HDFs transfected with COL1A1 plasmid (COL1A1 mRNA–encapsulating nanovesicles); ROS, reactive oxygen species; COL1A1, collagen type I alpha 1 chain.

Article Snippet: The concentration of human type I procollagen was measured using a human type I procollagen ELISA kit (Elabscience Biotechnology, China) according to the manufacturer's instructions.

Techniques: Derivative Assay, Transfection, Plasmid Preparation

Journal: Materials Today Bio

Article Title: Microcarriers encapsulating COL1A1 mRNA-loaded nanovesicles for skin photoaging treatment

doi: 10.1016/j.mtbio.2026.103126

Figure Lengend Snippet: In vivo biosafety evaluation. a) In vivo imaging of subcutaneous injection sites across different groups. b) Quantification of relative fluorescence intensity (n = 5). c) Histological analysis via H&E staining of the heart, liver, spleen, lung, and kidney tissues. d) ELISA quantification of IL-6 levels and TNF-α levels (n = 5). e) Quantification of ALT, AST, CREA, and UA levels (n = 3). The scale bar is 300 μm in c).

Article Snippet: The concentration of human type I procollagen was measured using a human type I procollagen ELISA kit (Elabscience Biotechnology, China) according to the manufacturer's instructions.

Techniques: In Vivo, In Vivo Imaging, Injection, Fluorescence, Staining, Enzyme-linked Immunosorbent Assay