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(A) LCS staining performance achieved using type-A gelatin from different commercial manufacturers. Red arrows indicate black granular precipitates. All scale bars = 50 μm. (B) LCS staining performance achieved <t>using</t> <t>type-B</t> gelatin from different commercial manufacturers. All scale bars = 50 μm.
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(A) LCS staining performance achieved using type-A gelatin from different commercial manufacturers. Red arrows indicate black granular precipitates. All scale bars = 50 μm. (B) LCS staining performance achieved using type-B gelatin from different commercial manufacturers. All scale bars = 50 μm.

Journal: bioRxiv

Article Title: A Rapid 10-Minute Silver Nitrate Staining Method for Visualizing the Osteocyte Lacuno-Canalicular System

doi: 10.64898/2026.04.20.719546

Figure Lengend Snippet: (A) LCS staining performance achieved using type-A gelatin from different commercial manufacturers. Red arrows indicate black granular precipitates. All scale bars = 50 μm. (B) LCS staining performance achieved using type-B gelatin from different commercial manufacturers. All scale bars = 50 μm.

Article Snippet: 0.5 mol/L EDTA decalcification solution (pH7.2, G1105-500ML, Servicebio, Wuhan), 10% EDTA decalcification solution(diluted from 0.5 mol/L EDTA), 14% EDTA decalcification solution(diluted from 0.5 mol/L EDTA), 5% Formic acid decalcification solution (G1107-500ML, Servicebio, Wuhan), 10% Neutral formalin fixative(311010014, Wexis, Guangzhou), 1 mol/L Silver nitrate solution(P1929026, Bolinda Technology, Shenzhen), Silver nitrate power (C510027-0010, Sangon Biotech, Shanghai), Type-B Galetin (G8061, bloom ∼225 g, Solarbio, Beijing), Type-A Galetin (A609764-0100, Sangon Biotech, Bloom 238∼282 g, Shanghai), Type-B Galetin (A600908-0500, Sangon Biotech, Shanghai), Type-A Galetin (S25197-100g, Yuanye Bio-Technology, Shanghai), Type-B Galetin (S22176-100g, Bloom ∼223 g, Yuanye Bio-Technology, Shanghai), Type-A Galetin (V900863-100G, Bloom ∼300 g, Sigma), Type-B Galetin (G9391-100G, Bloom ∼225 g, Sigma), 88% Formic acid (10010118, SinoPharm, Beijing).

Techniques: Staining