Review





Similar Products

two photon imaging  (Thorlabs)
86
Thorlabs two photon imaging
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Imaging, supplied by Thorlabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/microscope+photon+two/pmc13048257-315-1-4
Average 86 stars, based on 1 article reviews
two photon imaging - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
section two photon imaging system  (Vidrio Technologies)
86
Vidrio Technologies section two photon imaging system
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Section Two Photon Imaging System, supplied by Vidrio Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/laser+microscope+photon+scanning+two/bio_rxiv__2025__07__29__667237-380-14-23
Average 86 stars, based on 1 article reviews
section two photon imaging system - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
two photon calcium imaging  (Nikon)
99
Nikon two photon calcium imaging
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Calcium Imaging, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/Objectives/bio_rxiv__2025__07__20__665717-247-6-9
Average 99 stars, based on 1 article reviews
two photon calcium imaging - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
two-photon live imaging  (Nikon)
90
Nikon two-photon live imaging
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Live Imaging, supplied by Nikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/pmc09630450__41467_2022_34184_MOESM20_ESM-13-0-6
Average 90 stars, based on 1 article reviews
two-photon live imaging - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
two photon imaging  (Nikon)
99
Nikon two photon imaging
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Imaging, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/Objectives/bio_rxiv__2025__07__09__663340-209-2-4
Average 99 stars, based on 1 article reviews
two photon imaging - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier
two-photon laser scanning microscopy imaging  (TPLSM laboratories)
90
TPLSM laboratories two-photon laser scanning microscopy imaging
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Laser Scanning Microscopy Imaging, supplied by TPLSM laboratories, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/two+photon+laser+scanning+microscopy/pm40645580-403-2-6
Average 90 stars, based on 1 article reviews
two-photon laser scanning microscopy imaging - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
two-photon imaging system ultima  (Bruker Corporation)
90
Bruker Corporation two-photon imaging system ultima
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Imaging System Ultima, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/two+photon+microscope+ultima+iv/pm40614201-168-34-38
Average 90 stars, based on 1 article reviews
two-photon imaging system ultima - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier
two-photon imaging microscope  (Bruker Corporation)
90
Bruker Corporation two-photon imaging microscope
( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, <t>while</t> <t>two-photon</t> calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.
Two Photon Imaging Microscope, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/two-photon+imaging/two+photon+microscope/pm40512858-278-10-13
Average 90 stars, based on 1 article reviews
two-photon imaging microscope - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

Image Search Results


( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, while two-photon calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.

Journal: Science Advances

Article Title: Pupil-linked arousal heterogeneously modulates cell-type–specific sensory processing

doi: 10.1126/sciadv.adz6495

Figure Lengend Snippet: ( A ) Schematic of excitatory cortical projection neuron subtypes (left). Representative coronal sections of ACtx showing GCaMP8s expression (green) and 4′,6-diamidino-2-phenylindole (DAPI)–labeled (blue) nuclei (right). The transgenic and viral strategies noted in (B) were used to target L2/3, L5 IT, L5 ET, or CT neurons. WM, white matter. Scale bar, 250 μm. ( B ) Overview of the experimental design. Mice passively listen to pure tone frequencies, while two-photon calcium imaging and pupillometry were performed simultaneously. Cell-type–specific targeting strategies (top) used a combination of viral and/or transgenic methods. WT, wild type. Exemplar two-photon fields of view (FOVs) of each target ACtx cell subpopulation (right). Scale bar, 250 μm. ( C ) Exemplar 5-min recording showing pupil diameter (top; black), deconvolved neural activity from a single ET neuron (middle; orange), and sound onset times (bottom). Pupil size is expressed as a percentage of maximum diameter; neural activity is plotted in SDs ( z -scored) from baseline. ( D ) Histogram of trialwise pupil states across all mice. Counts refer to number of trials. Median pupil size of 62% is denoted by the yellow dashed line.

Article Snippet: All two-photon imaging (Bergamo, Thorlabs) was of the right ACtx.

Techniques: Expressing, Labeling, Transgenic Assay, Imaging, Activity Assay