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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive <t>for</t> <t>TTF‐1</t> (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.
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Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive for TTF‐1 (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.

Journal: The Journal of Pathology: Clinical Research

Article Title: The prognostic significance of the IASLC grading system in ALK ‐positive invasive non‐mucinous adenocarcinoma of the lung: correlation with clinicopathological features

doi: 10.1002/2056-4538.70093

Figure Lengend Snippet: Histological and immunohistochemical features of the ALK‐positive INMA cases (A) Examples of weak or strong ALK positivity (middle and right), with the left panel demonstrating negative staining. (B) H&E‐stained sections demonstrating histological patterns: lepidic (left), acinar (middle), and papillary (right). (C) Representative H&E‐stained sections showing cribriform (left), micropapillary (middle), and complex glandular (right) patterns. (D) H&E staining of an example of solid growth pattern (left); IHC staining shows tumor cells positive for TTF‐1 (middle) and negative for p40 (right). (bar = 100 μm). ALK, anaplastic lymphoma kinase; H&E, hematoxylin and eosin; IHC, immunohistochemistry; INMA, invasive non‐mucinous adenocarcinoma.

Article Snippet: The antibodies used included: ALK (790‐4797, ready‐to‐use, Ventana®), TTF‐1 (11833669A, 1:100, Zhongshan Golden Bridge Biotechnology, Beijing, China), p40 (25081519, 1:100, Dako, Carpinteria, CA, USA), Ki‐67 ( GT209429 , 1:100, Gene Tech Company Limited, Shanghai, China), PD‐L1 (SK006, ready‐to‐use, Dako, Carpinteria, CA, USA), CD4 (IR649, 1:100, Dako, Carpinteria, CA, USA), CD8 (ZA‐0508, 1:100, Zhongshan Golden Bridge Biotechnology, Beijing, China), and FoxP3 (sc‐53876, 1:100, Santa Cruz, Dallas, TX, USA).

Techniques: Immunohistochemical staining, Negative Staining, Staining, Immunohistochemistry