Journal: Scientific Reports

Article Title: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

doi: 10.1038/s41598-018-23930-1

Figure Lengend Snippet: Fluorescence signal from VZV62 dT-FAM probes. ( A ) Signal profile obtained after converting the Outer (VZV62F3), Inner (VZV62FIP) and Loop (VZV62LPB) primers into dT-FAM fluorescent probes. Input template concentration pVZV-ORF62 plasmid: 1 × 10 6 copies/µL. ( B ) Variation of fluorescence signal intensity due to varying levels of the Base Mix ranging from 0.5X, 1.0X, 1.5X, 2.0X and 2.5X. Input template concentration pVZV-ORF62 plasmid: 1 × 10 6 copies/µL. Water was used as a negative control. Each Time[30 sec interval] unit (X-axis) represents 30 seconds on the ESEQuant instrument.

Article Snippet: The instrument was programed to acquire the fluorescence signal every 30 sec. Amplification was flagged positive by the ESEQuant TS2 studio software ver 1.17.0 (Qiagen) software when any signal attained slope value, >20 mV from the baseline (0 mV).

Techniques: Fluorescence, Concentration Assay, Plasmid Preparation, Negative Control

Journal: Scientific Reports

Article Title: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

doi: 10.1038/s41598-018-23930-1

Figure Lengend Snippet: Optimization of signal intensity using the VZV62LPB-FAM probe. ( A ) Effect of increasing levels of F3/B3 primer pair from 0.5 µM (Mix 5), 1.0 µM (Mix 6) and 2.0 µM (Mix 7 OPT ). ( B ) Variation in signal intensity with increasing levels of the VZV62LPB-FAM probe from 0.1 µM, 0.2 µM and 0.3 µM. Input template concentration of pVZV-ORF62 plasmid: 1 × 10 6 copies/µL. Each Time[30 sec interval] unit (X-axis) represents 30 seconds on the ESEQuant instrument.

Article Snippet: The instrument was programed to acquire the fluorescence signal every 30 sec. Amplification was flagged positive by the ESEQuant TS2 studio software ver 1.17.0 (Qiagen) software when any signal attained slope value, >20 mV from the baseline (0 mV).

Techniques: Concentration Assay, Plasmid Preparation

Journal: Scientific Reports

Article Title: Real-time Detection and Monitoring of Loop Mediated Amplification (LAMP) Reaction Using Self-quenching and De-quenching Fluorogenic Probes

doi: 10.1038/s41598-018-23930-1

Figure Lengend Snippet: Detection of VZV from DFA positive clinical samples using VZV62 FLOS-LAMP assay. VZV DFA-positive clinical samples detected by the VZV62 FLOS-LAMP assay when ( A ) clinical samples were added directly into the reaction mixture and ( B ) after rapid DNA extraction using InstaGene™ matrix. Each Time[30 sec interval] unit (X-axis) represents 30 seconds on the ESEQuant instrument.

Article Snippet: The instrument was programed to acquire the fluorescence signal every 30 sec. Amplification was flagged positive by the ESEQuant TS2 studio software ver 1.17.0 (Qiagen) software when any signal attained slope value, >20 mV from the baseline (0 mV).

Techniques: Lamp Assay, DNA Extraction