Journal: Scientific Reports

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo

doi: 10.1038/s41598-017-09518-1

Figure Lengend Snippet: High-level expression and purification of TRAIL-Trimer fusion protein. ( a ) 10% SDS-PAGE analysis of TRAIL-Trimer expression from a fed-batch serum-free cell culture in the bioreactor. 10 µL of cell-free conditioned medium from Day 6 to Day 13 were analyzed under non-reducing condition followed by Coomassie Blue staining. ( b ) Bioassay analysis of TRAIL-Trimer production in conditioned medium from Day 5 to Day 13. ( c ) SDS-PAGE and western blot analysis of purified TRAIL-Trimer under either non-reducing or reducing conditions. 2 µg of purified protein was analyzed by a 10% SDS-PAGE and stained with Coomassie Blue. 0.2 µg of purified protein was analyzed by western blot using monoclonal antibody against TRAIL-domain and Trimer-domain, respectively.

Article Snippet: Bioactivity of purified TRAIL-Trimer, TRAIL-Fc and native TRAIL was evaluated using a Real-Time Cell Analysis (RTCA) iCELLigence system (ACEA Biosciences, San Diego, CA, USA) based on electrical impedance measurement which is a label-free approach for cell-based assays .

Techniques: Expressing, Purification, SDS Page, Cell Culture, Staining, Western Blot

Journal: Scientific Reports

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo

doi: 10.1038/s41598-017-09518-1

Figure Lengend Snippet: Purity evaluation of TRAIL-Trimer, TRAIL-Fc and native TRAIL. SDS-PAGE analysis of purified TRAIL-Trimer, TRAIL-Fc and native TRAIL under either non-reducing or reducing conditions. 2 µg of purified protein was analyzed by 10% or 15% SDS-PAGE, respectively, and stained with Coomassie Blue.

Article Snippet: Bioactivity of purified TRAIL-Trimer, TRAIL-Fc and native TRAIL was evaluated using a Real-Time Cell Analysis (RTCA) iCELLigence system (ACEA Biosciences, San Diego, CA, USA) based on electrical impedance measurement which is a label-free approach for cell-based assays .

Techniques: SDS Page, Purification, Staining

Journal: Scientific Reports

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo

doi: 10.1038/s41598-017-09518-1

Figure Lengend Snippet: High-level expression and purification of TRAIL-Trimer fusion protein. ( a ) 10% SDS-PAGE analysis of TRAIL-Trimer expression from a fed-batch serum-free cell culture in the bioreactor. 10 µL of cell-free conditioned medium from Day 6 to Day 13 were analyzed under non-reducing condition followed by Coomassie Blue staining. ( b ) Bioassay analysis of TRAIL-Trimer production in conditioned medium from Day 5 to Day 13. ( c ) SDS-PAGE and western blot analysis of purified TRAIL-Trimer under either non-reducing or reducing conditions. 2 µg of purified protein was analyzed by a 10% SDS-PAGE and stained with Coomassie Blue. 0.2 µg of purified protein was analyzed by western blot using monoclonal antibody against TRAIL-domain and Trimer-domain, respectively.

Article Snippet: The purity of TRAIL-Trimer was determined by both SDS-PAGE and SEC-HPLC (Sepax Zenix-C SEC. 300,Newark, DE, USA).

Techniques: Expressing, Purification, SDS Page, Cell Culture, Staining, Western Blot

Journal: Scientific Reports

Article Title: Improvement of Pharmacokinetic Profile of TRAIL via Trimer-Tag Enhances its Antitumor Activity in vivo

doi: 10.1038/s41598-017-09518-1

Figure Lengend Snippet: Purity evaluation of TRAIL-Trimer, TRAIL-Fc and native TRAIL. SDS-PAGE analysis of purified TRAIL-Trimer, TRAIL-Fc and native TRAIL under either non-reducing or reducing conditions. 2 µg of purified protein was analyzed by 10% or 15% SDS-PAGE, respectively, and stained with Coomassie Blue.

Article Snippet: The purity of TRAIL-Trimer was determined by both SDS-PAGE and SEC-HPLC (Sepax Zenix-C SEC. 300,Newark, DE, USA).

Techniques: SDS Page, Purification, Staining