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tRNA derived fragments <t>(tRFs)</t> transcriptome <t>sequencing</t> and target gene prediction. ( A ) The average length of identified tRFs from 6 libraries. ( B ) Heat map diagram of differentially expressed tRFs between normal and obese rats. ( C ) The list of top 10 differentially expressed tRFs between normal and obese rats. ( D ) tRF78576 cleaved from tRNA-Glu-TTC, which occupied 67.43% of fragments derived from tRNA-Glu-TTC ( E ) tRF GluTTC has complementary sequences with 3’UTR of Kruppel-like factor (KLF) 12, KLF12, KLF9, and KLF11. ( F ) The inhibitory effect of tRF GluTTC on target genes measured by luciferase assays. All data were presented as means ± standard deviation. * p < 0.05.
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tRNA derived fragments (tRFs) transcriptome sequencing and target gene prediction. ( A ) The average length of identified tRFs from 6 libraries. ( B ) Heat map diagram of differentially expressed tRFs between normal and obese rats. ( C ) The list of top 10 differentially expressed tRFs between normal and obese rats. ( D ) tRF78576 cleaved from tRNA-Glu-TTC, which occupied 67.43% of fragments derived from tRNA-Glu-TTC ( E ) tRF GluTTC has complementary sequences with 3’UTR of Kruppel-like factor (KLF) 12, KLF12, KLF9, and KLF11. ( F ) The inhibitory effect of tRF GluTTC on target genes measured by luciferase assays. All data were presented as means ± standard deviation. * p < 0.05.

Journal: Biomolecules

Article Title: tRNA-Derived Small Non-Coding RNAs as Novel Epigenetic Molecules Regulating Adipogenesis

doi: 10.3390/biom9070274

Figure Lengend Snippet: tRNA derived fragments (tRFs) transcriptome sequencing and target gene prediction. ( A ) The average length of identified tRFs from 6 libraries. ( B ) Heat map diagram of differentially expressed tRFs between normal and obese rats. ( C ) The list of top 10 differentially expressed tRFs between normal and obese rats. ( D ) tRF78576 cleaved from tRNA-Glu-TTC, which occupied 67.43% of fragments derived from tRNA-Glu-TTC ( E ) tRF GluTTC has complementary sequences with 3’UTR of Kruppel-like factor (KLF) 12, KLF12, KLF9, and KLF11. ( F ) The inhibitory effect of tRF GluTTC on target genes measured by luciferase assays. All data were presented as means ± standard deviation. * p < 0.05.

Article Snippet: Six adipose tissue samples were sent to Annoroad Gene Technology Co. Ltd. (Beijing, China) for tRFs sequencing.

Techniques: Derivative Assay, Sequencing, Luciferase, Standard Deviation