Journal: Development (Cambridge, England)

Article Title: Hemidesmosomes and Notch signaling regulate epidermal differentiation via delamination

doi: 10.1242/dev.205210

Figure Lengend Snippet: Notch signaling regulates integrin-β4 levels and delamination. (A-D) Confocal images of E17 Cre-negative control versus Rosa NICD epidermis (A) and E16 WT littermate versus Rbpj cKO epidermis (C), with single channel images of integrin-β4 on the right, and associated quantification of fluorescence intensity (B,D); n =3 except for WT control in Rbpj cohort ( n =2). (E-G) Images of Notch reporter (NR) transgenic (E) and LUGGIGE NR-transduced epidermis (F), and associated quantification (G). NR shown in green; RFP marks cells transduced with reporter. (H) Quantification of nuclear YAP in Itgb4 4124 epidermis. (I,K) Confocal images of E17 Cre-negative control versus Rosa NICD epidermis (I) and E16 WT littermate versus Rbpj cKO epidermis (K); asterisks indicate dual-positive cells. (J,L) Quantification of dual-positive cells in WT versus mutant. Each dot represents a biological replicate in G,H,J,L, or FOV in B,D, where shapes designate litters. Basement membrane is indicated with cyan dashed line. Scale bars: 25 μm (A,C,E,F,I,K); 10 µm (F, insets). ns, not significant; * P <0.05; **** P <0.0001 ( t -test).

Article Snippet: Transgenic Notch reporter animals were obtained from The Jackson Laboratory [ Tg(Cp-EGFP)25Gaia/J, stock #005854].

Techniques: Negative Control, Fluorescence, Control, Transgenic Assay, Transduction, Mutagenesis, Membrane

Journal: iScience

Article Title: Scanning Bessel beam microscopy with a protected and corrective objective for solvent-cleared large samples

doi: 10.1016/j.isci.2026.116358

Figure Lengend Snippet: High-resolution autofluorescence imaging of CUBIC-R-cleared E15.5 mouse embryos and Thy1-YFP-H mouse brains (A) Maximum intensity projection of a whole CUBIC-R-cleared E15.5 mouse embryo. (B) Surface rendering of a Thy1-YFP-H transgenic mouse brain. (C) Whole-brain image with depth-coded fluorescence intensity. A magnified inset shows the labeled neuron. (D) Surface rendering of the E15.5 mouse embryo. (E–G) Digital sections extracted from the 3D image along the sagittal (E), coronal (F), and transverse (G) planes. Scale bars: 1 mm.

Article Snippet: Thy1-YFP-H transgenic mice (Jackson strain No. 003782), Fos2A-iCreER knockin mice (Trap2; Jackson strain No. 030323), , and Ai75 reporter mice (Jackson strain No. 025106) were obtained from The Jackson Laboratory.

Techniques: Imaging, Transgenic Assay, Fluorescence, Labeling