Journal: Bioactive Materials

Article Title: Integrated apoptotic extracellular vesicle-recruitment peptide coating reprograms the diabetic bone microenvironment and orchestrates enhanced implant osseointegration

doi: 10.1016/j.bioactmat.2026.05.059

Figure Lengend Snippet: In vivo immunomodulatory effects of different modified surfaces in a DM model. (A) Schematic representation of the animal modeling and experimental treatment workflow. (B, C) hematoxylin and eosin staining of the peri-implant tissues in the femurs of DM rats 1 week after implantation, accompanied by quantitative analysis of the fibrous capsule thickness (scale bar = 100 μm, n = 5). (D–G) Immunofluorescence staining evaluating the polarization state of macrophages surrounding the implants (green: macrophage marker cluster of differentiation (CD) 68; red: M1 marker CD86 and M2 marker CD206; blue: nuclei), along with corresponding quantitative analysis of the fluorescence signals (scale bar = 100 μm, n = 5). (H–K) Immunohistochemical staining assessing the expression of the pro-inflammatory marker tumor necrosis factor-α and the anti-inflammatory marker interleukin-10 in the peri-implant area, with quantitative results of the positive staining areas (scale bar = 100 μm, n = 5). Data are expressed as the mean ± standard deviation, with statistical analysis performed using one-way ANOVA and Tukey's post-hoc test. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001 indicate statistical significance.

Article Snippet: For IHC analysis, sections underwent heat-induced antigen retrieval and blocking prior to incubation with antibodies against TNF-α (GB11188, Servicebio, China), IL-10 (GB11534, Servicebio, China), and VEGF (GB15165, Servicebio, China) to identify differences in local inflammatory and angiogenic profiles.

Techniques: In Vivo, Modification, Staining, Immunofluorescence, Marker, Fluorescence, Immunohistochemical staining, Expressing, Standard Deviation

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: ZH reduces IMQ-induced inflammatory responses and abnormal proliferation in mice. A Representative spleen images and spleen weight quantification. B Immunohistochemical (IHC) staining of Ki67 in skin lesions (left panel) and quantification of its AOD (right panel). C qRT-PCR analysis of Il-22 , Il-1β , Tnf-α , and Ccl20 mRNA expression in lesion skin tissues. D ELISA quantification of IL-22, IL-1β, TNF-α, and CCL20 protein levels in lesion skin tissues. n = 6 (biological replicates). Data are analyzed using one-way ANOVA followed Tukey’s post hoc test. ** P < 0.01 vs. Control group; # P < 0.05; ## P < 0.01 vs. IMQ group

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Immunohistochemical staining, Immunohistochemistry, Quantitative RT-PCR, Analysis, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: ZH alters gut microbiota composition and function in IMQ-induced mice. A Random forest analysis ranking bacterial taxa based on their importance in group classification using the Gini index. B Relative abundances of the top-ranked taxa across different groups. C Pearson correlation analysis between significantly altered bacterial taxa and skin inflammatory cytokines, including IL-22, IL-1β, TNF-α, and CCL20. * P < 0.05, ** P < 0.01

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Analysis

Journal: Archives of Microbiology

Article Title: Zhuhuang Granules alleviate psoriatic inflammation via restoring gut microbiota balance and enhancing intestinal barrier integrity

doi: 10.1007/s00203-026-05231-8

Figure Lengend Snippet: Gut microbiota depletion attenuates the therapeutic effects of ZH against IMQ-induced psoriasis-like inflammation. A Schematic diagram of the experimental design for antibiotic-mediated gut microbiota depletion and subsequent establishment of the IMQ-induced psoriasis-like dermatitis model. B Quantification of fecal bacterial abundance by qPCR to assess the efficacy of antibiotic-mediated gut microbiota depletion. C Representative images of dorsal skin lesions in mice from the indicated groups. D PASI scores for scaling, erythema, thickness, and total scores. E Representative images of spleens (left) and quantification of spleen weight (right). F Representative images of H&E-stained dorsal skin sections (left) and quantification of epidermal thickness (right). G Representative immunohistochemical staining of Ki67 in dorsal skin lesions (left) and quantification of Ki67-positive cells (right). H Relative mRNA expression levels of Il-22 , Il-1β , Tnf-α , and Ccl20 in dorsal skin lesions determined by qPCR. I Protein levels of IL-22, TNF-α, IL-1β, and CCL20 in dorsal skin lesions determined by ELISA. For panels B, the Control and ABX groups each contained 12 mice before randomization; for panels C-I, mice were randomly allocated into four groups ( n = 6 per group): IMQ, IMQ + ABX, IMQ + M-ZH, and IMQ + M-ZH + ABX. Data are analyzed using one-way ANOVA followed Tukey’s post hoc test. * P < 0.05, ** P < 0.01 vs. IMQ group; # P < 0.05, ## P < 0.01 vs. IMQ + ABX group; & P < 0.05, && P < 0.01 vs. IMQ + M-ZH group

Article Snippet: The concentrations of IL-22 (Cat. #E-EL-M2446, Elabscience, Wuhan, China), TNF-α (Cat. #CSB-E04741m, Cusabio, Wuhan, China), IL-1β (Cat. #CSB-E08054m, Cusabio), and CCL20 (Cat. #CSB-E04668m, Cusabio) were quantified as per the protocols of the manufacturers.

Techniques: Staining, Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay, Control

Journal: BMC Pharmacology & Toxicology

Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

doi: 10.1186/s40360-026-01225-w

Figure Lengend Snippet: Epinephrine and/or chlorpromazine eliminate APAP-induced oxidative stress and inflammation and modulate the expression of the thioredoxin system in the liver. ( A ) and ( B ) demonstrate the hepatic levels of Nrf2 and TNF-α, respectively. ( C ), ( D ), and ( E ) show the effect of EP and CPZ on the thioredoxin system (Trx1/TrxR1) ( C ), thioredoxin-related proteins (TrxL1 and TrxIP) ( D ) mRNA and TrxR1 protein ( E ). Bars represent the mean (± SD) of 3 experiments. Means are compared by two-way ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, *** p < 0.0001, **** p < 0.0001, and “ns” is not significant

Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

Techniques: Expressing

Journal: BMC Pharmacology & Toxicology

Article Title: Epinephrine-mediated sensitization of thioredoxin 1 enhances chlorpromazine protection against acute hepatotoxicity

doi: 10.1186/s40360-026-01225-w

Figure Lengend Snippet: Epinephrine and EGCG effect on cell viability, Nrf2, NTF, and TrxR1 expression in hepatoma cells. HepG2 and Huh7 cells were treated with epinephrine ( A ) or EGCG ( B ) for 24 h, after which cell metabolic activity was determined by MTT assay. Epinephrine enhanced cell viability in both HepG2 and Huh7 cells, whereas EGCG reduced cell viability. ( C ), ( D ), and ( E ) show the protein levels of Nrf2, TNF-α, and TrxR1, respectively, assessed in cells treated with EP, EGCG, CPZ, EP/CPZ, or EGCG/CPZ. Bars represent the mean (± SD) of 3 experiments. Groups were compared using ANOVA followed by Tukey’s tests. (*): p < 0.05, ** p < 0.01, (***): p < 0.001, (****): p < 0.0001, and “ns” is not significant

Article Snippet: Reduced Glutathione (GSH) Colorimetric Assay Kit (Cat. No. E-BC-K030-M), Human Nuclear Factor, Erythroid Derived 2, Like 2 (NFE2L2) ELISA Kit (Cat No. E-EL-H1564), Human TrxR1 (Thioredoxin Reductase 1) ELISA Kit (Cat. No. E-EL-H1728), human TNF-α ELISA Kit (Cat. No. E-EL-H0109) were from Elabscience, Houston, Tx, USA.

Techniques: Expressing, Activity Assay, MTT Assay