Review



tlr5  (Proteintech)


Bioz Verified Symbol Proteintech is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Proteintech tlr5
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Tlr5, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5/product/Proteintech
    Average 94 stars, based on 17 article reviews
    tlr5 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D"

    Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

    Journal: Frontiers in Cellular and Infection Microbiology

    doi: 10.3389/fcimb.2025.1616796

    Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Figure Legend Snippet: Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Techniques Used: Permeability, Expressing, Confocal Microscopy

    Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Figure Legend Snippet: Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Techniques Used: Expressing



    Similar Products

    tlr5  (Shanghai Korain Biotech Co Ltd)
    94
    Shanghai Korain Biotech Co Ltd tlr5

    Tlr5, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5/product/Shanghai Korain Biotech Co Ltd
    Average 94 stars, based on 1 article reviews
    tlr5 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tlr5 inhibitor  (MedChemExpress)
    94
    MedChemExpress tlr5 inhibitor

    Tlr5 Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5 inhibitor/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tlr5 inhibitor - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tlr5 elisa kit  (Shanghai Korain Biotech Co Ltd)
    93
    Shanghai Korain Biotech Co Ltd tlr5 elisa kit

    Tlr5 Elisa Kit, supplied by Shanghai Korain Biotech Co Ltd, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5 elisa kit/product/Shanghai Korain Biotech Co Ltd
    Average 93 stars, based on 1 article reviews
    tlr5 elisa kit - by Bioz Stars, 2026-03
    93/100 stars
    		  Buy from Supplier
    tlr5  (MedChemExpress)
    94
    MedChemExpress tlr5

    Tlr5, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tlr5 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tlr5  (Proteintech)
    94
    Proteintech tlr5
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Tlr5, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5/product/Proteintech
    Average 94 stars, based on 1 article reviews
    tlr5 - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tlr5 antibody  (Proteintech)
    94
    Proteintech tlr5 antibody
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Tlr5 Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5 antibody/product/Proteintech
    Average 94 stars, based on 1 article reviews
    tlr5 antibody - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    gene exp tlr5 hs00152825 m1  (Thermo Fisher)
    91
    Thermo Fisher gene exp tlr5 hs00152825 m1
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Gene Exp Tlr5 Hs00152825 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gene exp tlr5 hs00152825 m1/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    gene exp tlr5 hs00152825 m1 - by Bioz Stars, 2026-03
    91/100 stars
    		  Buy from Supplier
    tlr5 agonist  (MedChemExpress)
    94
    MedChemExpress tlr5 agonist
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Tlr5 Agonist, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5 agonist/product/MedChemExpress
    Average 94 stars, based on 1 article reviews
    tlr5 agonist - by Bioz Stars, 2026-03
    94/100 stars
    		  Buy from Supplier
    tlr5 agonist used at  (InvivoGen)
    96
    InvivoGen tlr5 agonist used at
    Intestinal permeability and expression of flagellin and <t>TLR5</t> protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)
    Tlr5 Agonist Used At, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tlr5 agonist used at/product/InvivoGen
    Average 96 stars, based on 1 article reviews
    tlr5 agonist used at - by Bioz Stars, 2026-03
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet:

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Control

    This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet: This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Generated

    (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet: (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Incubation, Generated, Expressing, Control

    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet:

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Control

    This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet: This figure shows all the H-bond interactions between robust CMap-generated leads and TLR5 which fall below 3 Å. The minimum 3 H-bond interactions between CMap-generated leads and TLR5 were showed by both Glide module and HADDOCK tool in each complex.

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Generated

    (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

    Journal: bioRxiv

    Article Title: Transcriptome-based lead generation, ligand- and structure-based prioritization and experimental validation of TLR5-activating molecules

    doi: 10.64898/2026.02.25.707690

    Figure Lengend Snippet: (A) CAL 27 cells were incubated with the CMap-generated leads: Cytarabine (200µM, 100µM, 50µM), Azacytidine (200µM, 100µM, 50µM), Penicillin (200µM, 100µM, 50µM), Streptozotocin (400µM, 200µM, 100µM), Piceatannol (400µM, 200µM, 100µM) and flagellin (5ng/ml) for 24h. (B) CAL 27 cells were incubated with the CMap-generated leads: Fenoterol (400µM, 200µM, 100µM), ABT-751 (200µM, 100µM, 50µM), Ganciclovir (200µM, 100µM, 50µM), Kinetin-riboside (100µM, 50µM, 25µM), and flagellin (5ng/ml) for 24h. Bar graph representing the relative expression levels of TLR5 with respect to the untreated control. Fold change is calculated by the signal of the treated sample divided by the signal of the control. If the fold change is >1, TLR5 expression is upregulated whereas fold change <1 is taken as downregulated. All values are expressed as mean ± SEM.

    Article Snippet: TLR5 secreted in the culture supernatants were measured using Human Toll-like Receptor 5, TLR5 ELISA kit (BT LAB, #E0374Hu) according to the manufacturer’s protocol.

    Techniques: Incubation, Generated, Expressing, Control

    Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

    doi: 10.3389/fcimb.2025.1616796

    Figure Lengend Snippet: Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

    Techniques: Permeability, Expressing, Confocal Microscopy

    Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

    doi: 10.3389/fcimb.2025.1616796

    Figure Lengend Snippet: Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

    Techniques: Expressing

    Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

    doi: 10.3389/fcimb.2025.1616796

    Figure Lengend Snippet: Intestinal permeability and expression of flagellin and TLR5 protein in the lamina propria of the mucosa of rats in various groups. FITC-Dextran was used to detect the intestinal permeability of rats and laser confocal microscopy was employed to observe the localization of flagellin and TLR5 proteins of colon three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

    Techniques: Permeability, Expressing, Confocal Microscopy

    Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: The effect of Massa Medicata Fermentata on the cytokine secretion of colonic mucosa and visceral sensitivity in rats with IBS-D

    doi: 10.3389/fcimb.2025.1616796

    Figure Lengend Snippet: Expression of TLR5, TRIF and p-EPK1/2 in LPDCs of rats in each group. The mRNA expression of TLR5, TRIF, and p-EPK1/2 in LPDCs was detected by qPCR. The expression of TLR5, TRIF, and p-EPK1/2 proteins in LPDCs of rats in each group was detected by WB three repeated tests. (Adopt Tukey statistical method. Refer to the annotations in the reference image for grouping identification.)

    Article Snippet: MMF (China Resources Sanjiu Co., Ltd); glacial acetic acid (10000208 Sinopharm, Inc.); FITC-Dextran (Sigma, Inc.); WB Antibodies: TLR5 Antibody (rabbit source, Proteintech, Inc.), TRIF Antibody (rabbit source, Affinity, Inc.); P-ERK1/2 Antibody (rabbit source, abcam, Inc.); immunofluorescent antibodies: immunofluorescent primary antibody: TLR5 (rabbit source, Three Eagles, Inc.), immunofluorescent primary antibody: Flic (mouse source, Yiqiao, Inc.), and Immunofluorescence secondary antibody: goat anti-rabbit IgGH&L (Cy3) (abcam, Inc.), immunofluorescence secondary antibody: goat anti-mouse IgGH&L (FITC) (abcam, Inc.); Immunohistochemistry antibody: immunohistochemistry primary antibody: TLR5 (rabbit source, proteintech, Inc.), immunohistochemistry primary antibody: TRIF (rabbit source, Affinity, Inc.), the Immunohistochemical primary antibody: p-ERK1/2 (rabbit source abcam), immunohistochemical secondary antibody: HRP-labeled goat anti-rabbit IgG (Biyun Tian, Inc.); Percoll lymphocyte isolate P8370 (Solarbio, Inc.); LPDCs magnetic bead sorting kit (OX62) (MiltenyiBiotec, Inc.) Anti-RatCD103, PE (Bioscience, Inc.); Rat spleen lymphocyte isolate LTS1083PK-200 (TBD, Inc.); CD4 Magnetic Bead Sorting Kit (MiltenyiBiotec, Inc.); CD4MonoclonalAntibody;CCK-8 Kit C0039 (Biotronix, Inc.); Rat Interleukin 12 (IL-12) ELISA Kit, Rat Interferon Gamma (IFN-γ) ELISA Kit, Rat Interleukin 4 (IL-4) ELISA Kit, Rat Interleukin 9 (IL-9) kit, Rat Interleukin 6 (IL-6) ELISA kit, Rat Interleukin 17A (IL-17A) ELISA kit, Rat Transforming Growth Factor β (TGF-β) ELISA kit (Jiangsu Jingmei, Inc.).

    Techniques: Expressing