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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with <t>Lectin</t> (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.
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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with <t>Lectin</t> (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.
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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with <t>Lectin</t> (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.
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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with <t>Lectin</t> (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.
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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with <t>Lectin</t> (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.
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PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with Lectin (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.

Journal: Journal of Ginseng Research

Article Title: Panaxadiol promotes angiogenesis against chronic cerebral hypoperfusion injury through the VEGF-A/p38 MAPK/Src signaling pathway

doi: 10.1016/j.jgr.2025.10.001

Figure Lengend Snippet: PD therapy promotes angiogenesis in the hippocampus of BCAS mice. (A) Schematic presentation of coronal section of mouse brain tissue. (B and C) Representative immunofluorescence images and vascular density quantification graph. Vessels are labeled with Lectin (green), and nuclei are labeled with DAPI (blue). (D) Pearson correlation between vessels density and CBF. (E–G) Pearson correlation between blood vessels numbers and scores from (E) MWM test, (F) NOR test, or (G) Y-maze assay on day 30 post-PD treatment. (H and I) Representative immunofluorescence images and BrdU-positive vascular number quantification graph. ECs are labeled with CD31 (red), proliferation cells are labeled with BrdU (green), and nuclei are labeled with DAPI (blue). (J) Pearson correlation between hippocampal BrdU-positive vessel density and CBF. Scale bar = 100 μm, One-way ANOVA with Tukey post hoc test was used. Each data represent mean ± SEM, n = 3, ∗p < 0.05, ∗∗∗∗p < 0.0001 vs sham + NS group.

Article Snippet: Biotinylated Lycopersicon esculentum (tomato) lectin (tomato-lectin; B-1175, Vector Laboratories, Burlingame, CA, USA; 1.25 mg/kg) was administered via transcardial injection 5 min prior to sacrifice as described [ ].

Techniques: Immunofluorescence, Labeling