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Image Search Results


Summary of commercially available, first-generation, multigene prognosticators in breast cancer.

Journal: ecancermedicalscience

Article Title: The past and future of breast cancer treatment—from the papyrus to individualised treatment approaches

doi: 10.3332/ecancer.2017.746

Figure Lengend Snippet: Summary of commercially available, first-generation, multigene prognosticators in breast cancer.

Article Snippet: Currently, several of these first-generation prognostic gene signatures are clinically available: (i) Endopredict (Sividon Diagnostics, Cologne, Germany) (ii) MammaPrint (Agendia, Amsterdam, the Netherlands), (iii) MapQuant DX (Ispogen, Marseille, France), (iv) Oncotype DX (Genomic Health, CA, USA), (v) PAM50 (Nanostring Technologies, WA, USA), (vi) Theros (bioTheranostics, CA, USA), (v) ( ).

Techniques:

Fabrication and characterization of the MN-MSC patch. a) Schematic of MN-MSC patch fabrication process by a1) casting, a2) blue light crosslinking, a3) peeled off from PDMS mold and adding GelMA solution with MSCs, a4) blue light crosslinking. b) SEM image of b1) interstructure of normal and b2) porous GelMA hydrogel. Scale bars of images (b1, b2) indicate 300µm, and scale bars of images (b3, b4) indicate 100um. c) Calcein (live)/EthD (dead) staining revealed the morphology and viability of MSC encapsulated in GelMA hydrogel on c1) day 3 and c2) day 5. Scale bars: 200 μm. d) The quantitative viability analysis of MSC encapsulated in GelMA hydrogel on days 1, 3 and 5. Data were analyzed with one-way ANOVA followed by Tukey’s post hoc test. n=4. *p<0.05. e) Representative optical microscopy images and f-g) SEM (scanning electron microscope) images of MN. Scale bars for images f) indicate 500 µm, and scale bars for images g) indicate 50 µm. h) The mechanical strength of MN. i) Schematic showing the study design used to test the releasing profile of exosomes from the MN-MSC patch. j) The daily exosome release curves of MN-MSC patch fabricated with porous and normal GelMA hydrogel. Data are presented as means ± SD (n = 3 for each group). k) The daily exosome release curves of MN-MSC and MN-EXO patch. Data are presented as means ± SD (n = 3 for each group).

Journal: bioRxiv

Article Title: Porous microneedle patch with sustained exosomes delivery repairs severe spinal cord injury

doi: 10.1101/2022.07.18.500400

Figure Lengend Snippet: Fabrication and characterization of the MN-MSC patch. a) Schematic of MN-MSC patch fabrication process by a1) casting, a2) blue light crosslinking, a3) peeled off from PDMS mold and adding GelMA solution with MSCs, a4) blue light crosslinking. b) SEM image of b1) interstructure of normal and b2) porous GelMA hydrogel. Scale bars of images (b1, b2) indicate 300µm, and scale bars of images (b3, b4) indicate 100um. c) Calcein (live)/EthD (dead) staining revealed the morphology and viability of MSC encapsulated in GelMA hydrogel on c1) day 3 and c2) day 5. Scale bars: 200 μm. d) The quantitative viability analysis of MSC encapsulated in GelMA hydrogel on days 1, 3 and 5. Data were analyzed with one-way ANOVA followed by Tukey’s post hoc test. n=4. *p<0.05. e) Representative optical microscopy images and f-g) SEM (scanning electron microscope) images of MN. Scale bars for images f) indicate 500 µm, and scale bars for images g) indicate 50 µm. h) The mechanical strength of MN. i) Schematic showing the study design used to test the releasing profile of exosomes from the MN-MSC patch. j) The daily exosome release curves of MN-MSC patch fabricated with porous and normal GelMA hydrogel. Data are presented as means ± SD (n = 3 for each group). k) The daily exosome release curves of MN-MSC and MN-EXO patch. Data are presented as means ± SD (n = 3 for each group).

Article Snippet: The morphology of the MNs was assessed with a scanning electron microscope (SEM) (Thero FEI, Nova Nano 450).

Techniques: Staining, Microscopy