Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Characterization of ADSC EVs and UC-MSC EVs. (a) Size and concentration measurement by NTA; (b) Morphology visualization with Cryo-TEM with EVs indicated by red arrows (scale bar: 100 nm); (c) EV related biomarkers in the EV groups and cell lysate (CL) detected with Western blot, including positive markers TSG101, HSP70, CD63 and negative marker calnexin.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques: Concentration Assay, Western Blot, Marker

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Bulk transcriptomic analysis of a reconstructed skin model after treatment with ADSC EVs and UC-MSC EVs. (a,b) Volcano plots and heatmaps showing commonly DEGs regulated by high and low doses of (a) ADSC EVs and (b) UC-MSC EVs, with 5-8 replicates per group; (c,d) Top GO terms enriched among DEGs modulated by (c) ADSC EVs and (d) UC-MSC EVs, as identified in the GO database.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques:

Journal: Frontiers in Cell and Developmental Biology

Article Title: Extracellular vesicles modulate skin aging biomarkers in a 3D reconstructed full-thickness skin model

doi: 10.3389/fcell.2026.1784998

Figure Lengend Snippet: Comprehensive profiling of miRNA and protein cargo in EVs. (a) Venn diagram illustrating miRNAs in the two groups of EVs; (b) Top abundantly expressed miRNAs identified in each EV types; (c) Overlap between predicted target genes of shared miRNAs and DEGs from T-skin™ transcriptomic analysis; (d) Identified proteins in the two groups of EVs revealed by Venn plot; (e) Top expressed proteins in the two types of EVs; (f) Selected DEPs that upregulated in ADSC EVs compared to UC-MSC EVs.

Article Snippet: The EVs used in this study were produced by EchoBiotech (Beijing, China), using commercial human cell lines UC-MSC (ATCC #PCS-500-010) and ADSC (ATCC #PCS-500-011).

Techniques:

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 1 Recombinant adeno-associated virus (rAAV)-mediated enhancement of VEGF165 facilitates Schwann cell differentiation in hADSCs. (A) Distinct green fluorescence was observed within the cytoplasm of both control ADSCs and VEGF165 ADSCs after infection, indicating high infection efficiency. (B) Cell viability of hADSCs measured by the MTT assay, expressed as % viability compared to the blank group. (C) Representative inverted microscope image of hADSCs after 14 days of Schwann cell induction. Without induction, hADSCs exhibited a mesh-like structure, while induced cells showed a spindle shape with reduced volume, fewer protrusions, and a spiral growth pattern resembling Schwann cells. Notably, the VEGF165 group exhibited more pronounced Schwann cell-like features compared to the blank and control groups. (D–F) Analysis of VEGF165 gene and protein expression in undifferentiated and differentiated hADSCs by quantitative real-time polymerase chain reaction (qRT- PCR, D) and Western blotting (E,F), respectively. Experimental groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as the mean ± SD of three independent experiments. * indicates significance compared to undifferentiated hADSCs. # indicates significance compared to the blank and control groups at p < 0.05.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Cell Differentiation, Fluorescence, Control, Infection, MTT Assay, Inverted Microscopy, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 2 Investigating Schwann cell-related genes in hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Analysis of S100B, NGFR, and GFAP expressions in differentiated hADSCs following rAAV-mediated VEGF165 modification and 14-day culture in Schwann cell medium. Quantitative real-time polymerase chain reaction (qRT-PCR, A–C) and Western blotting (D–G) were performed. The study groups include the blank group (without infection), control group (rAAV control infection), and VEGF165 group (rAAV VEGF165 infection). Data are presented as mean ± SD of three independent experiments. * indicates significance compared to the blank and control groups at p < 0.05.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Modification, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Infection, Control

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 3 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on motor function in a sciatic nerve injury model. (A) Footprint analysis assessed gait dynamics from 1 to 4 weeks post-surgery, using hind paws coated in red ink to measure print length (PL), toe spread (TS), and intermediary toe spread (IT). (B) Sciatic function index (SFI) analysis. * indicates significance compared to the PBS group. # indicates significance compared to the hADSCs and control-hADSCs groups. n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Control

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 4 Impact of recombinant adeno-associated virus (rAAV)-mediated VEGF165 in hADSCs on nerve regeneration in a sciatic nerve injury model. (A) Histological examination utilizing toluidine blue staining. (B) Morphometric analysis of fiber diameter (FD), axon diameter (AD), and myelin thickness. * indicates significant difference compared to the PBS group (p < 0.05). # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Recombinant, Virus, Staining, Control

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 5 VEGF165-hADSCs injection elevated expression of Schwann cell markers in the distal nerve segment at 4 weeks post-surgery. (A–C) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of S100 calcium-binding protein B (S100B), nerve growth factor receptor (NGFR), and glial fibrillary acidic protein (GFAP) gene expression. (D–G) Western blotting assessment of S100B, NGFR, and GFAP protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control-hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Injection, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Binding Assay, Gene Expression, Western Blot, Control

Journal: The Kaohsiung journal of medical sciences

Article Title: Enhancing therapeutic potential: Human adipose-derived mesenchymal stem cells modified with recombinant adeno-associated virus expressing VEGF165 gene for peripheral nerve injury.

doi: 10.1002/kjm2.12875

Figure Lengend Snippet: FIGURE 6 (A,B) Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of brain-derived neurotrophic factor (BDNF) and glial cell-derived neurotrophic factor (GDNF) gene expression. (C–E) Western blotting assessment of mature BDNF and GDNF protein expression. * indicates significant difference compared to the PBS group (p < 0.05); # indicates significant difference compared to the hADSCs and control- hADSCs groups (p < 0.05). n = 6 animals/group.

Article Snippet: The normal human ADSCs (hADSCs) (Catalog: PCS-500-011, ATCC, USA) are fibroblast-like cells cultured in mesenchymal stem cell basal medium (Catalog: PCS-500-030, ATCC, USA) supplemented with a mesenchymal stem cell growth kit (Catalog: PCS-500-040, ATCC, USA).

Techniques: Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Derivative Assay, Gene Expression, Western Blot, Expressing, Control