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3D reconstructions of LKB1 heterotrimeric complex are colored by local resolution. To the right of each structure is a close-up view for a representative α-helix fit into each density map. Overall Q-scores and Q-score derived effective resolution are shown below each structure and the representative helix. In (A) and (B) are reconstructions <t>from</t> <t>TFS</t> <t>Glacios</t> microscope with the Alpine detector at 120 keV and 200 keV respectively. In (C) and (D) are reconstructions from the K3 detector collections on TFS Glacios at 200 keV and TFS Titan Krios at 300 keV respectively.
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3D reconstructions of LKB1 heterotrimeric complex are colored by local resolution. To the right of each structure is a close-up view for a representative α-helix fit into each density map. Overall Q-scores and Q-score derived effective resolution are shown below each structure and the representative helix. In (A) and (B) are reconstructions from TFS Glacios microscope with the Alpine detector at 120 keV and 200 keV respectively. In (C) and (D) are reconstructions from the K3 detector collections on TFS Glacios at 200 keV and TFS Titan Krios at 300 keV respectively.

Journal: bioRxiv

Article Title: High-resolution single particle imaging at 100-200 keV with the Gatan Alpine direct electron detector

doi: 10.1101/2024.02.14.580363

Figure Lengend Snippet: 3D reconstructions of LKB1 heterotrimeric complex are colored by local resolution. To the right of each structure is a close-up view for a representative α-helix fit into each density map. Overall Q-scores and Q-score derived effective resolution are shown below each structure and the representative helix. In (A) and (B) are reconstructions from TFS Glacios microscope with the Alpine detector at 120 keV and 200 keV respectively. In (C) and (D) are reconstructions from the K3 detector collections on TFS Glacios at 200 keV and TFS Titan Krios at 300 keV respectively.

Article Snippet: To test the practical application of lower accelerating voltage electron detection by the Gatan Alpine sensor, we imaged apoferritin with the Alpine direct detector on a TFS Glacios microscope equipped with a narrow pole piece set to an accelerating voltage of 120 kV.

Techniques: Derivative Assay, Microscopy