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Journal: STAR Protocols
Article Title: Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice
doi: 10.1016/j.xpro.2026.104537
Figure Lengend Snippet: Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.
Article Snippet:
Techniques: Amplification, Purification, Control, In Vitro, Reverse Transcription
Journal: STAR Protocols
Article Title: Protocol to produce and apply barcoded rabies virus for single-neuron input mapping in mice
doi: 10.1016/j.xpro.2026.104537
Figure Lengend Snippet: Workflow for barcode amplicon library preparation This figure summarizes the workflow for barcode amplicon library preparation in this part. The left panel shows barcode enrichment from scRNA-seq cDNA by two rounds of PCR, followed by bead purification and quality control to generate the final Illumina barcode library. The middle panel shows spike-in RNA preparation, including spike-in template assembly, purification, in vitro transcription, DNA digestion, RNA purification, and quality control. The right panel shows input region library preparation, including reverse transcription, pooling and purification, pre-amplification PCR, and quality control to generate the final Illumina input-region library. Step numbers in red correspond to the detailed protocol steps.
Article Snippet:
Techniques: Amplification, Purification, Control, In Vitro, Reverse Transcription