Journal: Cell Death & Disease

Article Title: FGFR4 promotes CAF activation through the CXCL10-CXCR3 axis in colon cancer

doi: 10.1038/s41419-025-07588-y

Figure Lengend Snippet: A Collagen gel contraction assay results show the effect of recombinant CXCL10 protein on the contractility of NIH/3T3 cells (n = 6/group). Representative gel images are shown together with the quantification of collagen gel contraction. B Migration and invasion assay results show the effect of recombinant CXCL10 protein on the migration and invasion properties of NIH/3T3 cells. The effect of CXCL10 was compared to that of TGFβ, which induces CAF activation. C The effect of recombinant CXCL10 protein (ng) on CAF marker gene levels in NIH/3T3 cells was determined using western blotting and RT-qPCR analyses. D Effect of Cxcl10 knockdown on CAF marker gene expression. NIH/3T3 cells were incubated for 24 h with conditioned media (CM) obtained from siNC- or siCXCL10-transfected CT-26/FGFR4 cells. Western blotting and RT-qPCR analyses of CAF marker expression in NIH/3T3 cells. E Effect of CXCL10 neutralizing antibody on CAF marker gene expression. NIH/3T3 cells were incubated in CM obtained from CT-26/FGFR4 cells in the presence of either normal anti-IgG or anti-CXCL10 neutralizing antibodies (concentrations shown in μg/mL) for 24 h. F Effect of AMG487, a pharmacological CXCR3 inhibitor, on gene expression of CAF markers. The CM of CT-26/FGFR4 cells, alone or in combination with the CXCR3 inhibitor, was used to treat the NIH/3T3 cells for 24 h. G Invasion assay results depicting the effect of the CXCR3 inhibitor AMG487 (2 μM) on the invasion capabilities of CT-26 primary tumor-derived CAFs. H Invasion assay results show the effects of various CXCR3 inhibitors on the invasive properties of CT26/FGFR4 tumor-derived CAFs. The CXCR3 inhibitors used (at 2 μM) were: TAK779, NBI74330, and SCH546738. Data are presented as mean ± SD. Statistical significance: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001 using Student’s t -test and one-way ANOVA). NT no treatment, AMG AMG487, TAK TAK779, NBI NBI74330, SCH SCH54673 .

Article Snippet: The following inhibitors were used: BLU9931 (Merck, Kenilworth, NJ, USA; 538776), AMG487 (MedChemExpress, Monmouth Junction, NJ, USA; HY-15319), TAK779 (MedChemExpress; HY-13406), NBI74330 (MedChemExpress; HY-15320), and SCH546738 (MedChemExpress; HY-10017).

Techniques: Collagen Gel Contraction Assay, Recombinant, Migration, Invasion Assay, Activation Assay, Marker, Western Blot, Quantitative RT-PCR, Knockdown, Gene Expression, Incubation, Transfection, Expressing, Derivative Assay

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the locomotor activities in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote a significant difference from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote a significant difference from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the maximum grip strength at week 6 (A) and week 12 (B) in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on body weight, food intake and total muscle weight in BALB/c mice.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Control, Injection

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the plasma levels of cortisol at week 4 and week 12 ( A,B , respectively) and corticosterone at week 7 and week 11 ( C,D , respectively) in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Clinical Proteomics, Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the mRNA expression of CRH (A) in the hypothalamus and of POMC (B) in the pituitary gland in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Expressing, Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the plasma levels of MCP-1 at week 4 (A) and week 12 (B) in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Clinical Proteomics, Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the mRNA expression of MCP-1 (A) and CCR2 (B) in the brainstem tissues in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Expressing, Injection, Control

Journal: Frontiers in Nutrition

Article Title: Quercetin attenuates cisplatin-induced fatigue through mechanisms associated with the regulation of the HPA axis and MCP-1 signaling

doi: 10.3389/fnut.2025.1530132

Figure Lengend Snippet: The effect of cisplatin (CDDP) alone or in combination with quercetin or TAK779 on the levels of TNF- α (A) , IL-1β (B) , and IL-6 (C) and total quercetin (D) in the cerebrum tissues in BALB/c mice. Quercetin was administered by a diet containing 1% quercetin (OQ) or intraperitoneal injection (IQ). The control group received the control diet and vehicle only. Values are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001 denote significant differences from the control group; # p < 0.05, ## p < 0.01, ### p < 0.001 denote significant differences from the CDDP group.

Article Snippet: TAK779 was from AdooQ Bioscience (Irvine, CA, USA).

Techniques: Injection, Control

Journal: Cell stem cell

Article Title: BMI1 Inhibition Eliminates Residual Cancer Stem Cells after PD1 Blockade and Activates Antitumor Immunity to Prevent Metastasis and Relapse

doi: 10.1016/j.stem.2020.06.022

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: For the inhibition of CXCR3 and CXCR5, mice were given TAK779 (Sigma-Aldrich, Cat#SML0911, 150 μg/mouse twice/week).

Techniques: Recombinant, Microscopy, Protease Inhibitor, Cell Stimulation, SYBR Green Assay, Staining, Western Blot, Enzyme-linked Immunosorbent Assay, Picogreen Assay, Plasmid Preparation, Software