Journal: Translational Cancer Research

Article Title: Radiation-activated astrocytes promote tumor progression through CXCL12 secretion and induce resistance to anti-PD-1 immunotherapy

doi: 10.21037/tcr-2025-1-2652

Figure Lengend Snippet: Intracranial tumor transplantation experiment. (A) Survival duration of normal mice, mice subjected to cranial irradiation, mice with solely transplanted tumors, and mice with transplanted tumors following cranial irradiation (n=6). (B) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (C) IHC of CD4, CD8, CD56, PD1, and Ki67 in peritumoral tissue and tumor tissue. a, brain tissue from the normal mouse group; b, brain tissue from the mouse group subjected to radiotherapy; c, peritumoral brain tissue from the mouse group with tumor induction alone; d, peritumoral brain tissue from the mouse group with tumor induction followed by radiotherapy; e, tumor tissue from the mouse group with tumor induction alone; f, tumor tissue from the mouse group with tumor induction followed by radiotherapy. (D) Bar chart of immunohistochemistry (n=6). (E) Survival time of mice with solely transplanted tumors and mice with transplanted tumors following cranial irradiation after receiving anti-PD-1 therapy (n=6). (F) The MRI scan clearly illustrates the size of the tumor, and the tumor is outlined by a red dashed line. (G) Kill rate analysis of GL261 glioma cells co-cultured with CD8 + T cells. Scale bars: 40 µm for IHC images. ns, not significant; *, P<0.05; **, P<0.01; ***, P<0.001. IHC, immunohistochemistry; MRI, magnetic resonance imaging; PD-1, programmed cell death protein 1.

Article Snippet: First, CD8 + T cells were cultured in CD8 + T cell complete medium (Procell, CM-M380) and then activated using IL-2 and CD3/CD28 antibodies.

Techniques: Transplantation Assay, Irradiation, Immunohistochemistry, Cell Culture, Magnetic Resonance Imaging