Journal: Journal of cancer therapy

Article Title: Breast Cancer MCF-7 Cell Spheroid Culture for Drug Discovery and Development

doi: 10.4236/jct.2022.133009

Figure Lengend Snippet: Effect of breast cancer drugs on MCF-7 Spheroid. 10 μ M of drugs was used for the treatment. MCF-7 spheroid was treated after 1 day of spheroid development. CON = ethanol control, STX = STX64 (Irosustat), QUE = quercetin, TAM = tamoxifen.

Article Snippet: STX64 (STX, Irosustat, Cat. No BD767626) was ordered from BLD Pharmatech Co., Limited.

Techniques: Control

Journal: Molecular and cellular endocrinology

Article Title: Adrenal Androgens Rescue Prostatic Dihydrotestosterone Production and Growth of Prostate Cancer Cells after Castration

doi: 10.1016/j.mce.2019.02.018

Figure Lengend Snippet: DHT production by prostate tissue ex vivo was STS-dependent. Inserted tables showed the exact values of DHT production, in each treatment presented in the respective bar graph at the top in the format of mean and range (lowest value, highest value). DHEAS 3.5 μM and STX64 5 μM were used in the combination treatments.

Article Snippet: The STS inhibitor STX64 was purchased from Sigma-Aldrich.

Techniques: Ex Vivo

Journal: Molecular and cellular endocrinology

Article Title: Adrenal Androgens Rescue Prostatic Dihydrotestosterone Production and Growth of Prostate Cancer Cells after Castration

doi: 10.1016/j.mce.2019.02.018

Figure Lengend Snippet: DHEAS activation of AR in LAPC-4 and VCaP cell lines was diminished by AR antagonist bicalutamide (Bica) and STX64. T 1 nM, Bicalutamide 10 μM, DHEAS 3.5 μM, and STX64 5 μM were used in the combination treatments. Cells were treated for 24 hr. * p < 0.05 compared to T 1 nM. # p < 0.05 compared to DHEAS 3.5 μM.

Article Snippet: The STS inhibitor STX64 was purchased from Sigma-Aldrich.

Techniques: Activation Assay

Journal: Molecular and cellular endocrinology

Article Title: Adrenal Androgens Rescue Prostatic Dihydrotestosterone Production and Growth of Prostate Cancer Cells after Castration

doi: 10.1016/j.mce.2019.02.018

Figure Lengend Snippet: AR-dependent stimulation of growth by adrenal androgens. (A) DHEAS and DHEA stimulated growth of VCaP cells. (B) DHEAS (DS) stimulated growth was diminished by AR antagonist bicalutamide (Bica) and STX64 (B). Cells were treated for 3 days and 7 days. Growth was assessed using MTT assay. Data were presented in percentage to untreated controls at each time point (day). * p < 0.05 compared to control. # p < 0.05 compared to DHEAS.

Article Snippet: The STS inhibitor STX64 was purchased from Sigma-Aldrich.

Techniques: MTT Assay