Journal: Scientific Reports

Article Title: Centrally mediated responses to NMES are influenced by muscle group and stimulation parameters

doi: 10.1038/s41598-024-75145-2

Figure Lengend Snippet: Experimental protocol. The different stimulation frequencies and pulse durations are displayed in the ascending order but were in fact randomized. MVC = maximal voluntary contraction; NMES = neuromuscular electrical stimulation.

Article Snippet: Then, single stimuli (monophasic pulses of 1 ms at 400 V) were delivered using a stimulation pen (Compex, Ecublens, Switzerland) to precisely localize the motor points of the different muscles, i.e. the site where the strongest response was induced by the weakest current .

Techniques:

Journal: Scientific Reports

Article Title: Centrally mediated responses to NMES are influenced by muscle group and stimulation parameters

doi: 10.1038/s41598-024-75145-2

Figure Lengend Snippet: Position of the participant and placement of the stimulation and electromyographic (EMG) electrodes on each muscle group (elbow flexors, knee extensors, plantar flexors from left to right). Arrows represent the direction of force production.

Article Snippet: Then, single stimuli (monophasic pulses of 1 ms at 400 V) were delivered using a stimulation pen (Compex, Ecublens, Switzerland) to precisely localize the motor points of the different muscles, i.e. the site where the strongest response was induced by the weakest current .

Techniques:

Journal: Scientific Reports

Article Title: Centrally mediated responses to NMES are influenced by muscle group and stimulation parameters

doi: 10.1038/s41598-024-75145-2

Figure Lengend Snippet: A Original traces of evoked force from a representative subject for plantar flexors (PF, upper panels), knee extensors (KE, middle panels) and elbow flexors (EF, lower panels). Force traces are presented for each pulse duration and frequency condition. B Original traces of sustained electromyographic (EMG) from a representative subject for the soleus (left), vastus lateralis (middle) and biceps brachii (right). EMG traces are presented for each pulse duration and frequency condition. Stimulation artifacts always precede sustained EMG activity, when visible.

Article Snippet: Then, single stimuli (monophasic pulses of 1 ms at 400 V) were delivered using a stimulation pen (Compex, Ecublens, Switzerland) to precisely localize the motor points of the different muscles, i.e. the site where the strongest response was induced by the weakest current .

Techniques: Activity Assay

Journal: Scientific Reports

Article Title: Centrally mediated responses to NMES are influenced by muscle group and stimulation parameters

doi: 10.1038/s41598-024-75145-2

Figure Lengend Snippet: Comparison of extra force ( A ) and sustained electromyographic (EMG) activity ( B ) between muscle groups (PF: plantar flexors; KE: knee extensors; EF: elbow flexors) at each stimulation frequency. Individual and mean data (red) are displayed. For extra force, positive values representing responders are in black while negative values corresponding to non-responders are in grey. EMG activity was measured on soleus for plantar flexors, vastus lateralis for knee extensors and biceps brachii for elbow flexors. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Then, single stimuli (monophasic pulses of 1 ms at 400 V) were delivered using a stimulation pen (Compex, Ecublens, Switzerland) to precisely localize the motor points of the different muscles, i.e. the site where the strongest response was induced by the weakest current .

Techniques: Comparison, Activity Assay

Journal: Scientific Reports

Article Title: Centrally mediated responses to NMES are influenced by muscle group and stimulation parameters

doi: 10.1038/s41598-024-75145-2

Figure Lengend Snippet: Effect of stimulation frequency on extra force and sustained EMG activity by muscle group.

Article Snippet: Then, single stimuli (monophasic pulses of 1 ms at 400 V) were delivered using a stimulation pen (Compex, Ecublens, Switzerland) to precisely localize the motor points of the different muscles, i.e. the site where the strongest response was induced by the weakest current .

Techniques: Activity Assay

Journal: Frontiers in Veterinary Science

Article Title: Comparative analysis of superovulated versus uterine-embryo synchronized recipients for embryo transfer in cynomolgus monkeys ( Macaca fascicularis )

doi: 10.3389/fvets.2024.1452631

Figure Lengend Snippet: Timeline of experimental procedures by date. (A) Schedule of superovulated recipients. (B) Schedule of synchronized recipients. GnRH-a, gonadotrophin-releasing hormone antagonist; hCG, human chorionic gonadotrophin; hFSH, human follicle-stimulating hormone; hMG, human menopausal gonadotrophin.

Article Snippet: The regimen included the administration of a gonadotropin-releasing hormone (GnRH) antagonist, ganirelix (Orgalutran Inj, ORGANON, Seoul, Korea), at a dosage of 0.125 mg once daily, and recombinant human follicle-stimulating hormone (hFSH) (Gonal-F Pen, Merck, Serono, Italy) at 37.5 IU twice daily intramuscularly on days 1–6.

Techniques:

Journal: STAR Protocols

Article Title: Calcium measurements in enzymatically dissociated or mechanically microdissected mouse primary skeletal muscle fibers

doi: 10.1016/j.xpro.2023.102260

Figure Lengend Snippet:

Article Snippet: CRITICAL: We use a pen-type stimulation electrode connected to a generic current pulse stimulator with up to 100V stimulation voltage (World Precision Instruments; model no. SYS-A300 or Aurora Scientific; model no. 701C).

Techniques: Recombinant, Membrane, Fluorescence, Dissection

Journal: STAR Protocols

Article Title: Calcium measurements in enzymatically dissociated or mechanically microdissected mouse primary skeletal muscle fibers

doi: 10.1016/j.xpro.2023.102260

Figure Lengend Snippet:

Article Snippet: Pen-type stimulation electrode , ADInstruments , MLA0320.

Techniques: Recombinant, Membrane, Fluorescence, Dissection