Review




Structured Review

Cisbio Bioassays stimulating buffer
Stimulating Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/stimulation+buf/bio_rxiv__2024__12__31__630923-282-20-21
Average 90 stars, based on 1 article reviews
stimulating buffer - by Bioz Stars, 2026-09
90/100 stars

Images

Related Articles

Concentration Assay:

Article Title: Inhibiting a promiscuous GPCR: iterative discovery of bitter taste receptor ligands
Article Snippet: .. Then, the library compounds and controls, dissolved in DMSO, were prediluted to twofold final concentration into 96 deep-well plates (nerbe plus, Winsen/Luhe, Germany) containing stimulation buffer (Cisbio, Codolet, France) in a total volume of 333 μL and were subsequently transferred into the assay plates containing cells (total V = 20 μl/well). ..

Article Title: Inhibiting a promiscuous GPCR: iterative discovery of bitter taste receptor ligands
Article Snippet: .. Then, the library compounds and controls, dissolved in DMSO, were prediluted to 2-fold final concentration into 96 deep-well plates (nerbe plus, Winsen/Luhe, Germany) containing stimulation buffer (Cisbio, Codolet, France) in a total volume of 333 μL and were subsequently transferred into the assay plates containing cells (total V = 20 μl/well). ..

Incubation:

Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode.
Article Snippet: WT GPR1 and its mutants, and WT human CMKLR1 for comparison, were transiently expressed in HEK293T cells for 24 h. IP1 accumulation was tested using the IP-One Gq HTRF kit (Cisbio). .. The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37 ̊C. ..

Article Title: Bitter-Tasting Amino Acids l-Arginine and l-Isoleucine Differentially Regulate Proton Secretion via T2R1 Signaling in Human Parietal Cells in Culture.
Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.

Article Title: Rescue of mutant gonadotropin-releasing hormone receptor function independent of cognate receptor activity
Article Snippet: .. The plates were incubated for 17 h at 37 °C, 5% CO prior to addition of 3 μL of 1,000 nM GnRH in 2X stimulation buffer or stimulation buffer only (buffer provided in CisBio IP-One Kit). .. The plates were incubated for 2 h at 37 °C and 5% CO . 1.5 μL IP-One D2 (1:20 dilution—CisBio) and 1.5 μl IP-One Cryptate (1:20 dilution—CisBio) were added to each well and incubated for 1 h at room temperature in the dark.

Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode
Article Snippet: WT GPR1 and its mutants, and WT human CMKLR1 for comparison, were transiently expressed in HEK293T cells for 24 h. IP1 accumulation was tested using the IP-One Gq HTRF kit (Cisbio). .. The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C. ..

Transfection:

Article Title: Mechano-dependent signaling by Latrophilin/CIRL quenches cAMP in proprioceptive neurons
Article Snippet: For IP accumulation assays, the IP-One HTRF assay kit (CisBio) was used according to manufacturer ́s protocol. .. In brief, transfected COS-7 cells were washed 48 hr post transfection with PBS and subsequently stimulated with 1 mM peptide in stimulation buffer (CisBio) for 30 min at 37°C. ..

other:

Article Title: Bruton's Tyrosine Kinase Inhibitors with Distinct Binding Modes Reveal Differential Functional Impact on B-Cell Receptor Signaling
Article Snippet: Inositol phosphate 1 homogeneous time-resolved fluorescence assay Compounds were added in a 384-well low-volume plate using D300e digital dispenser (TECAN), and 50,000 Ramos cells (wildtype and BTK knockout) were incubated with compounds in cell growth medium for 4 hours before 10 mg/mL goat F(ab’)2 antihuman IgM (Southern Biotech, catalog no. 2022–01) was added in stimulation buffer (IP-One kit, Cisbio, catalog no. 40451).

Enzyme-linked Immunosorbent Assay:

Article Title: Bitter-Tasting Amino Acids l-Arginine and l-Isoleucine Differentially Regulate Proton Secretion via T2R1 Signaling in Human Parietal Cells in Culture.
Article Snippet: is published by the American Chemical Society.. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society.. Copyright © American Chemical Society.



Similar Products

99
Thermo Fisher stimulation buffer
Stimulation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/HEPES/pm24900623__ml400257h_si_001-67-2-10
Average 99 stars, based on 1 article reviews
stimulation buffer - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

90
Merck KGaA ip1 stimulation buffer
Ip1 Stimulation Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/ip1+stimulation+buffer/10__7554_slash_elife__98766-391-8-20
Average 90 stars, based on 1 article reviews
ip1 stimulation buffer - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Cisbio Bioassays stimulating buffer
Stimulating Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/stimulation+buf/bio_rxiv__2024__12__31__630923-282-20-21
Average 90 stars, based on 1 article reviews
stimulating buffer - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Cisbio Bioassays ip1 stimulation buffer
Ip1 Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/ip1+stimulation+buf/us12162864-928-14-17
Average 90 stars, based on 1 article reviews
ip1 stimulation buffer - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

90
Cisbio Bioassays stimulation buffer 4
Stimulation Buffer 4, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/stimulation+buf/bio_rxiv__2024__12__04__626849-232-21-27
Average 90 stars, based on 1 article reviews
stimulation buffer 4 - by Bioz Stars, 2026-09
90/100 stars
  Buy from Supplier

86
Cisbio Bioassays stimulation buffer
(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 <t>stimulation</t> based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
Stimulation Buffer, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/stimulation+buf/pmc11515964-125-6-8
Average 86 stars, based on 1 article reviews
stimulation buffer - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

99
Thermo Fisher camp stimulation buffer
(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 <t>stimulation</t> based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
Camp Stimulation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/Hanks'+Balanced+Salt+Solution/pm39147328-59-6-16
Average 99 stars, based on 1 article reviews
camp stimulation buffer - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

99
Thermo Fisher glucose stimulated insulin secretion krebs buffer
(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 <t>stimulation</t> based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .
Glucose Stimulated Insulin Secretion Krebs Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stimulation+buffer/D(%2B)-Glucose/us12054745-2607-0-34
Average 99 stars, based on 1 article reviews
glucose stimulated insulin secretion krebs buffer - by Bioz Stars, 2026-09
99/100 stars
  Buy from Supplier

Image Search Results


(A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

Journal: PLOS Biology

Article Title: Structure of G protein-coupled receptor GPR1 bound to full-length chemerin adipokine reveals a chemokine-like reverse binding mode

doi: 10.1371/journal.pbio.3002838

Figure Lengend Snippet: (A) cAMP inhibition mediated by GPR1 and CMKLR1 in response to different concentrations of chemerin and C9. (B) G protein dissociation assay based on NanoBiT technology in transfected cells that express GPR1 and CMKLR1, respectively, and stimulated with chemerin and C9 at different concentrations. Control: HBSS without ligand addition. (C) IP-one accumulation in cells expressing GPR1 and CMKLR1, respectively, in response to chemerin and C9 at different concentrations. (D) β-arrestin recruitment upon chemerin and C9 stimulation based on NanoBiT technology, in transfected cells expressing GPR1 and CMKLR1, respectively. Data shown are means ± SEM from 3 independent experiments. The underlying data can be found in .

Article Snippet: The cells were resuspended in the stimulation buffer (Cisbio) and incubated with different concentrations of C9 peptide diluted in the stimulation buffer for 30 min at 37°C.

Techniques: Inhibition, Transfection, Control, Expressing