Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: Structure of stevioside.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques:

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: Cell growth inhibition of stevioside on HT-29 colon cancer cells (A) MTT assay, (B) LDH assay. LDH, lactate dehydrogenase.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Inhibition, MTT Assay, Lactate Dehydrogenase Assay

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: (A) Apoptotic effect of stevioside on HT-29 colon cancer cells, (B) effect of stevioside on caspase-9 activity, (C) Effect of stevioside on caspase-3 activity. Data is shown as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Activity Assay, Standard Deviation

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: Cell cycle and apoptosis ratios with various concentrations of stevioside on HT-29 cells.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Concentration Assay

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: (A) Effect of stevioside on ROS production, (B) effect of stevioside on ΔΨm level. Data are shown as the mean ± standard deviation. *P<0.05, **P<0.01, ***P<0.001. ROS, reactive oxygen species; ΔΨm, mitochondrial membrane potential.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Standard Deviation

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: Regulation of p-p38 MAPK, pERK and β-actin expression in HT-29 cells upon stevioside treatment. p-p38 MAPK, phosphorylated P-38 mitogen-activated protein kinase pathway; pERK, phosphorylated extracellular signal-regulated kinases.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Expressing

Journal: Oncology Letters

Article Title: Stevioside induced cytotoxicity in colon cancer cells via reactive oxygen species and mitogen-activated protein kinase signaling pathways-mediated apoptosis

doi: 10.3892/ol.2017.5744

Figure Lengend Snippet: Growth inhibitory effect of stevioside on HT-29 cells subsequent to treatment with (A) NAC, an ROS scavenger, (B) the ERK inhibitor PD98059 and (C) the MAPK inhibitor SB203580. Data are shown as the mean ± standard deviation. *P<0.05, **P<0.01. S, stevioside; NAC, N-acetyl-L-cysteine; ROS, reactive oxygen species; ERK, extracellular signal-regulated kinases; MAPK, mitogen-activated protein kinase pathway.

Article Snippet: Stevioside hydrate (purity ≥98%), fetal bovine serum (FBS), MTT, propidium iodide and SDS were purchased from Sigma-Aldrich; Merck Millipore, Darmstadt, Germany.

Techniques: Standard Deviation