mouse phospho stat6 (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc mouse phospho stat6
Mouse Phospho Stat6, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 233 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stat6/Phospho-Stat6+(Tyr641)+Rabbit+mAb/pmc13049607-338-66-69
Average 96 stars, based on 233 article reviews

mouse phospho stat6 - by Bioz Stars, 2026-10

96/100 stars

Images

Ubiquitin Proteomics:

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence.
Article Snippet: Then rProtein A/G Beads (Cell Signaling, # 37478) were added and incubated for 4 h. The immunoprecipitated was washed three times with buffer and followed by immunoblot. .. Antibodies used were as follows: HSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized, 1:200), DNA-PK (Santa Cruz, #sc-390849, 1:500), ATM (Cell Signaling, #2873, 1:1000), ATR (Santa Cruz, #sc-515173, 1:500), CHK1 (Cell Signaling, #2360, 1:1500), CHK2 (Proteintech, #13954-1-AP, 1:1000), p16-INK4A (Abcam, #ab211542, 1:1000), γH2AX (Cell Signaling, #9718, 1:1500), BRCA1 (Thermo Fisher, #MA1-23164, 1:1000), UBE2T (Thermo Fisher, #PA576202, 1:1000), JAK (Cell Signaling, #50996, 1:1000), STING (Cell Signaling, #13647, AR TI CL E IN P RE SS 1:2000), STING (pS365) (Cell Signaling, #72971, 1:1000), pTBK1 (pS172) (Cell Signaling, #5483, 1:1000), pJAK1 (pY1034/1035) (Cell Signaling, #74129, 1:1000), Ubiquitin (Cell Signaling, #3936, 1:1000), K48-specific ubiquitin (Abcam, #ab140601, 1:1000), PU.1 (Abcam, #ab227835, 1:1500), Col1a1 (Cell Signaling, #72026, 1:1000), SMAD2/3 (Cell Signaling, #8685, 1:1000), pSMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, #8828, 1:1000), αSMA (Cell Signaling, #19245, 1:2000) anti-HA (Cell Signaling, #3724, 1:5000) and anti-Flag (Cell Signaling, #8146, 1:5000). .. Generation of STAT6 pS807-sepicific antibody To generate a polyclonal antibody specific for STAT6 phosphorylated at Ser807 (pS807), we collaborated with ABclonal Tech.

Article Title: DNA-PK-mediated phosphorylation of STAT6 establishes a non-canonical type 2 immunity axis to prevent macrophage senescence
Article Snippet: Then, rProtein A/G Beads (Cell Signaling, # 37478) were added and incubated for 4 h. The immunoprecipitated was washed three times with buffer and followed by immunoblot. .. Antibodies used were as follows: HSP90 (Santa Cruz, #sc-13119, 1:10,000), α-Tubulin (Sigma, #T6199, 1:10,000), STAT6 (Cell Signaling, #5397, 1:1500), pSTAT6(pY641) (Cell Signaling, #56554, 1:1000), pSTAT6(pS807) (ABclonal Tech, customized, 1:200), DNA-PK (Santa Cruz, #sc-390849, 1:500), ATM (Cell Signaling, #2873, 1:1000), ATR (Santa Cruz, #sc-515173, 1:500), CHK1 (Cell Signaling, #2360, 1:1500), CHK2 (Proteintech, #13954-1-AP, 1:1000), p16-INK4A (Abcam, #ab211542, 1:1000), γH2AX (Cell Signaling, #9718, 1:1500), BRCA1 (Thermo Fisher, #MA1-23164, 1:1000), UBE2T (Thermo Fisher, # PA576202 , 1:1000), JAK (Cell Signaling, #50996, 1:1000), STING (Cell Signaling, #13647, 1:2000), STING (pS365) (Cell Signaling, #72971, 1:1000), pTBK1 (pS172) (Cell Signaling, #5483, 1:1000), pJAK1 (pY1034/1035) (Cell Signaling, #74129, 1:1000), Ubiquitin (Cell Signaling, #3936, 1:1000), K48-specific ubiquitin (Abcam, #ab140601, 1:1000), PU.1 (Abcam, #ab227835, 1:1500), Col1a1 (Cell Signaling, #72026, 1:1000), SMAD2/3 (Cell Signaling, #8685, 1:1000), p-SMAD2 (Ser465/467)/SMAD3 (Ser423/425) (Cell Signaling, #8828, 1:1000), αSMA (Cell Signaling, #19245, 1:2000) anti-HA (Cell Signaling, #3724, 1:5000) and anti-Flag (Cell Signaling, #8146, 1:5000). .. To generate a polyclonal antibody specific for STAT6 phosphorylated at Ser807 (pS807), we collaborated with ABclonal Tech.

Incubation:

Article Title: TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6-GAS6 Axis.
Article Snippet: .. The membranes were blocked with 5% skim milk at room temperature for 1 h. Primary antibodies—TGM2 (Proteintech, 15100-1-AP, 1:20000), GAS6 (Proteintech, 13795-1- P, 1:1000), STAT6 (CST, #5397, 1:1000), p-STAT6 (CST, #56554, 1:1000), p-JAK1 (CST, #3331, 1:1000) and p-JAK3 (Proteintech, 29101-1-AP, 1:1000)—were incubated overnight at 4◦C. .. After washing with PBST, fluorescent secondary antibodies (LICOR) were added and incubated at room temperature for 1 h, followed by additional washing.

Article Title: TGM2 Aggravates Acute Pancreatitis by Impairing Macrophage Efferocytosis Through Inhibition of the STAT6-GAS6 Axis.
Article Snippet: .. Protein samples were incubated overnight at 4◦C on a rotating shaker with IP antibodies against the following targets: TGM2 (Proteintech, 15100-1-AP, 1:50), STAT6 (Cell Signaling Technology, #5397, 1:50), and IgG (Abcam, UK, 1:50). .. Protein A/G magnetic beads (Bimake, B23202, USA) were washed three times with RIPA buffer and then blocked with 5% BSA at 4◦C for 30 min.

Article Title: Multi-omics analysis reveals the association of cesarean delivery with altered gut microbial profiles and a Th2-biased immune response in neonates.
Article Snippet: Equal amounts of protein were mixed with 5× loading buffer (Cat# C05-03001, Bioss, USA), denatured by heating at 95–100 °C for 5–10 min, and separated on 10% SDS-PAGE gels (Cat# SPGT001, Biolight, China). .. After electrophoresis, proteins were transferred to a membrane, blocked, and probed with primary antibodies against STAT4 (Cat# 2653T, CST, USA) and STAT6 (Cat# 5397T, CST, USA), followed by incubation with appropriate HRP-conjugated secondary antibodies. .. Protein bands were detected using ECL substrate (Cat# C05-07004, Bioss,

Article Title: Zhi-Chuan-Ling alleviates OVA-induced allergic asthma by suppressing M2 macrophage polarization via the PI3K/AKT/mTOR/STAT6 pathway.
Article Snippet: .. The membranes were blocked with 5 % non-fat milk at room temperature for 2 h, and then incubated with primary antibodies against the following proteins at 4 ◦C overnight: PI3K (4292, Cell Signaling Technology, MA, USA), AKT (4691, Cell Signaling Technology, MA, USA), mTOR (66,888-1-Ig, Proteintech, Wuhan, China), STAT6 (5397, Cell Signaling Technology, MA, USA), pPI3K (17,466, Cell Signaling Technology, MA, USA), p-AKT (4060, Cell Signaling Technology, MA, USA), p-mTOR (80,596-1-RR, Proteintech, Wuhan, China), and p-STAT6 (56,554, Cell Signaling Technology, MA, USA), CD206 (24,595, Cell Signaling Technology, MA, USA). ..

Electrophoresis:

Membrane:

Western Blot:

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection.
Article Snippet: Protein concentrations were quantified using the BCA Protein Assay Kit (Thermo Fisher Scientific). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA). .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotech nology, USA).

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

Cell-Signaling:

Article Title: CXCL5 suppresses osteoclastogenesis and protects against lipoteichoic acid-induced bone loss by modulating PLCγ2 and c-Fos signalling in gram-positive periprosthetic joint infection
Article Snippet: .. Western blotting was performed using primary antibodies against the following targets: nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) (4389), phospho-phospholipase Cγ2 (PLCγ2) (Tyr1217, 3871T), PLCγ2 (3872T), phospho-PLCγ1 (Tyr783, 2821T), PLCγ1 (5690T), c-Fos (4384), calcineurin A (2614S), phospho-p38 mitogen-activated protein kinase (MAPK) (Thr180/Tyr182, 9211), p38 MAPK (8690), phospho-SAPK/JNK (Thr183/Tyr185, 4668), SAPK/JNK (9252), phospho-ERK1/2 (4370), ERK1/2 (9102), phospho-nuclear factor-kappa B (NF-κB) p65 (Ser536, 3033), NF-κB p65 (8242), phospho-signal transducer and activator of transcription 5 (STAT5) (Tyr694, 9359S), STAT5 (94205T), phospho-STAT6 (Tyr641, 56554S), STAT6 (5397S), phospho-FAK (Tyr397, 3283S), and FAK (3285T) (all from Cell Signaling Technology, USA); TRAP (ab191406) and CXCR2 (ab217314; Abcam); CTSK (sc-48353), tumour necrosis factor receptor-associated factor 6 (TRAF6; sc-8409), and β-actin (sc-47778; Santa Cruz Biotechnology, USA). .. Signal detection was carried out using enhanced chemiluminescence solution (Revvity, USA) and visualized with a UVP imaging system (Analytik Jena, Germany).

Review