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Chromatogram in two meconium samples testing positive for EtS. The retention time of substance ranged from 4.5 to 5.5 min depending on the column life span. The black line depicts the quantitative transitions and the blue line depicts the qualitative transitions. The green shade represents the quantification of EtS using the calibration curves. The “multiple reaction monitoring” (MRM) program takes into account the following transitions: EtS quantifier 125 > 80 and qualifier 125 > 97, and internal standard <t>(EtS-d5)</t> quantifier 130 > 98 and qualifier 130 > 80. ( a ) sample EMF_MEC_42 with a positive concentration of 10 ng/g; ( b ) sample EMF_MEC_12 with a positive concentration of 19.57 ng/g.
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Chromatogram in two meconium samples testing positive for EtS. The retention time of substance ranged from 4.5 to 5.5 min depending on the column life span. The black line depicts the quantitative transitions and the blue line depicts the qualitative transitions. The green shade represents the quantification of EtS using the calibration curves. The “multiple reaction monitoring” (MRM) program takes into account the following transitions: EtS quantifier 125 > 80 and qualifier 125 > 97, and internal standard <t>(EtS-d5)</t> quantifier 130 > 98 and qualifier 130 > 80. ( a ) sample EMF_MEC_42 with a positive concentration of 10 ng/g; ( b ) sample EMF_MEC_12 with a positive concentration of 19.57 ng/g.
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Chromatogram in two meconium samples testing positive for EtS. The retention time of substance ranged from 4.5 to 5.5 min depending on the column life span. The black line depicts the quantitative transitions and the blue line depicts the qualitative transitions. The green shade represents the quantification of EtS using the calibration curves. The “multiple reaction monitoring” (MRM) program takes into account the following transitions: EtS quantifier 125 > 80 and qualifier 125 > 97, and internal standard (EtS-d5) quantifier 130 > 98 and qualifier 130 > 80. ( a ) sample EMF_MEC_42 with a positive concentration of 10 ng/g; ( b ) sample EMF_MEC_12 with a positive concentration of 19.57 ng/g.

Journal: International Journal of Molecular Sciences

Article Title: Alcohol Consumption during Pregnancy: Analysis of Two Direct Metabolites of Ethanol in Meconium

doi: 10.3390/ijms17030417

Figure Lengend Snippet: Chromatogram in two meconium samples testing positive for EtS. The retention time of substance ranged from 4.5 to 5.5 min depending on the column life span. The black line depicts the quantitative transitions and the blue line depicts the qualitative transitions. The green shade represents the quantification of EtS using the calibration curves. The “multiple reaction monitoring” (MRM) program takes into account the following transitions: EtS quantifier 125 > 80 and qualifier 125 > 97, and internal standard (EtS-d5) quantifier 130 > 98 and qualifier 130 > 80. ( a ) sample EMF_MEC_42 with a positive concentration of 10 ng/g; ( b ) sample EMF_MEC_12 with a positive concentration of 19.57 ng/g.

Article Snippet: Sample preparation: 10 to 15 mg of the meconium were diluted with internal standards (EtG-d5 and EtS-d5; Medichem LGC Standards, Wesel, Germany).

Techniques: Targeted Proteomics, Concentration Assay