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dapi  (Miltenyi Biotec)


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    Miltenyi Biotec dapi
    Dapi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/DAPI+Staining+Solution/pm42855519-284-0-2
    Average 97 stars, based on 94 article reviews
    dapi - by Bioz Stars, 2026-11
    97/100 stars

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    Related Articles

    Incubation:

    Article Title: Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells
    Article Snippet: .. Samples were incubated on a shaker for 45 min and washed with phosphate buffered saline (PBS) prior staining with DAPI (1:3000) and the fluorescently conjugated endothelial cell marker, CD31 (1:400, Miltenyi-Biotec). .. For the adhesion assay in the MARC chambers, samples were fixed with 4% PFA, washed with HEPES buffered saline solution (HBSS), and stained with a nuclear stain 4′, 6-diamidino-2 phenylindole (DAPI, 1:3000, Life Technologies), and phalloidin 546 (1:300, Life Technologies).

    Saline:

    Article Title: Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells
    Article Snippet: .. Samples were incubated on a shaker for 45 min and washed with phosphate buffered saline (PBS) prior staining with DAPI (1:3000) and the fluorescently conjugated endothelial cell marker, CD31 (1:400, Miltenyi-Biotec). .. For the adhesion assay in the MARC chambers, samples were fixed with 4% PFA, washed with HEPES buffered saline solution (HBSS), and stained with a nuclear stain 4′, 6-diamidino-2 phenylindole (DAPI, 1:3000, Life Technologies), and phalloidin 546 (1:300, Life Technologies).

    Staining:

    Article Title: Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells
    Article Snippet: .. Samples were incubated on a shaker for 45 min and washed with phosphate buffered saline (PBS) prior staining with DAPI (1:3000) and the fluorescently conjugated endothelial cell marker, CD31 (1:400, Miltenyi-Biotec). .. For the adhesion assay in the MARC chambers, samples were fixed with 4% PFA, washed with HEPES buffered saline solution (HBSS), and stained with a nuclear stain 4′, 6-diamidino-2 phenylindole (DAPI, 1:3000, Life Technologies), and phalloidin 546 (1:300, Life Technologies).

    Marker:

    Article Title: Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells
    Article Snippet: .. Samples were incubated on a shaker for 45 min and washed with phosphate buffered saline (PBS) prior staining with DAPI (1:3000) and the fluorescently conjugated endothelial cell marker, CD31 (1:400, Miltenyi-Biotec). .. For the adhesion assay in the MARC chambers, samples were fixed with 4% PFA, washed with HEPES buffered saline solution (HBSS), and stained with a nuclear stain 4′, 6-diamidino-2 phenylindole (DAPI, 1:3000, Life Technologies), and phalloidin 546 (1:300, Life Technologies).

    other:

    Article Title: Evaluation of the topographical influence on the cellular behavior of human umbilical vein endothelial cells
    Article Snippet: For cell marker expression analysis, the HUVEC monolayers were washed with PBS with calcium and magnesium, fixed with 4% PFA before blocking with 10% goat serum (Thermo Fischer Scientific).



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing