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4 6 diamidino 2 phenylindole dapi staining solution  (Beyotime)


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    Structured Review

    Beyotime 4 6 diamidino 2 phenylindole dapi staining solution
    4 6 Diamidino 2 Phenylindole Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2079 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+solution/DAPI+Staining+Solution/pm42844620-90-10-14
    Average 99 stars, based on 2079 article reviews
    4 6 diamidino 2 phenylindole dapi staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Liquiritin Enhances Chemosensitivity to Doxorubicin in Breast Cancer by Promoting Ubiquitination-Mediated Degradation of MCL1.
    Article Snippet: Breast cancer (BC) represents a life‐threatening malignant disease that profoundly endangers women's health, with chemoresistance restricting treatment efficacy.. Liquiritin (LIQ) has shown great potential in cancer therapy, yet its effect on the chemosensitivity of BC cells remains unclear.. BC cells were treated with different concentration gradients of Doxorubicin (DOX) and LIQ.

    Article Title: Epigenetically silenced KAT2B suppresses de novo lipogenesis through destroying HDAC5/LSD1 complex assembly in renal cell carcinoma
    Article Snippet: .. Dead or apoptotic cells in the organoids were stained using Beyo3DTM YO-PRO-1 (C1356S, Beyotime, China) staining solution, following the manufacturer's instructions. .. Six organoids were randomly selected from each group to analyze the ratio of dead cell area to the total organoid area using ImageJ software.

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Article Title: Przewaquinone A regulates cell cycle and autophagy through the SrC/STAT3 signaling pathway to inhibit colorectal cancer progression.
    Article Snippet: Przewaquinone A (PrA), a natural active substance extracted from Salvia przewalskii Maxim, has been shown to have antitumor activity and can act as a STAT3 inhibitor to modulate the Src/STAT3 pathway.. However, its role in colorectal cancer (CRC), along with its related mechanisms, has not yet been clarified.. This study aims to explore whether PrA inhibits the malignant advancement of CRC via the Src/STAT3 pathway, and to provide new drug candidates and molecular targets for CRC clinical treatment.

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)

    CCK-8 Assay:

    Article Title: Degradation-Mediated Bioactive Calcium Release from Alginate Gel Fibers for Enhanced Bone Regeneration.
    Article Snippet: Biodegradable hydrogels are promising for bone regeneration but are limited by a significant mechanical mismatch with bone tissue in modulus, strength, and toughness.. To address the weakness, this work reported a mesh-like hydrogel woven from calcium-alginate gel fibers.. The fibers, manufactured via wet-spinning, possessed tensile strengths (36–285 MPa) and moduli (200 MPa–9.1 GPa) comparable to those of bone tissue.

    Incubation:

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Gentle:

    Article Title: RIPK3 overcomes 5-fluorouracil resistance in colorectal cancer cells via AKT/mTOR-mediated autophagy
    Article Snippet: .. For crystal violet staining, cells were incubated with staining solution (Beyotime, China) for 30 min, followed by gentle washing 3 times with PBS, air-drying, and final photography. .. Confluent cellular monolayers were established in six-well plates and transfected using Lipo 8000 transfection reagent (Beyotime Biotechnology, C0601).

    Infection:

    Article Title: Myeloperoxidase (MPO) mediates LPS-induced mitophagy in murine macrophages through AMPK/ULK1 pathway.
    Article Snippet: Neutrophils play a key role in innate immune system, taking up 50%70% of the whole circulating leukocytes, which are considered as the body's first line defense against pathogens.. Once reach the injured site, active neutrophils could release myeloperoxidase (MPO) through degranulation or neutrophil extracellular traps (NETs), making MPO a hallmark for neutrophil activation [1].. As a heme-containing peroxidase, MPO could kill pathogens through catalyzing H2O2 and halide ions (Cl , Br , I and SCN ) to produce oxidative hypohalides, among which hypochlorous acid (HOCl) is the main product in vivo [2,3].

    Microscopy:

    Article Title: Mechanistic study of locally transplanted bone marrow mesenchymal stem cells combined with erythropoietin in acute spinal cord injury.
    Article Snippet: .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.) staining solution and observed under a microscope. .. The sections were stained with hematoxylin and eosin (H&E, Beyotime, China.)

    other:

    Article Title: Inhibiting Arachidonate-5-lipoxygenase expression ameliorates osteoarthritis progression by suppressing ferroptosis via the JAK2/STAT3 signaling pathway.
    Article Snippet: Background: Osteoarthritis (OA) is a degenerative joint disease.. Recent studies have shown that ferroptosis plays a critical role in OA.. Arachidonate-5-lipoxygenase (ALOX5), a pivotal enzyme regulating arachidonic acid metabolism, is involved in the synthesis of the pro-inflammatory leukotrienes.



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    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    Beyotime giemsa staining
    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) <t>Representative</t> <t>JC-1</t> staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.
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    Image Search Results


    HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

    Journal: Journal of Inflammation Research

    Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

    doi: 10.2147/JIR.S623377

    Figure Lengend Snippet: HG and PA treatments induce ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs using DCFH-DA fluorescence probe in control RAW264.7 macrophages and those with treatments of 25 mmol/L HG and 60 μM PA for 48 h. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control and HG- and PA-induced RAW264.7 macrophages. ( C and D ) Measurement of MDA and GSH levels in control and HG- and PA-induced RAW264.7 macrophages. ( E ) Representative JC-1 staining photographs in control and HG- and PA-induced RAW264.7 macrophages. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantification MMP changes. ( G ) Detection of ATP in control and HG- and PA-induced RAW264.7 macrophages. ( H ) TEM for investigating the changes in mitochondrial morphology in control and HG- and PA-induced RAW264.7 macrophages. Scale bars, 500 nm. **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from Student’s t test.

    Article Snippet: After 24 h, 300 μL JC-1 staining solution (C2006; Beyotime) was added, with additional 20-min cultivation in the dark at 37°C.

    Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay

    Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

    Journal: Journal of Inflammation Research

    Article Title: HMGCS2 Palmitoylation Promotes Diabetes-Induced Erectile Dysfunction Through PPARα-Mediated Macrophage Extracellular Trap Formation

    doi: 10.2147/JIR.S623377

    Figure Lengend Snippet: Pharmacological suppression of protein palmitoylation attenuates HG- and PA-induced ROS over-production, oxidative stress, and mitochondrial dysfunction in macrophages. ( A ) Representative intracellular ROS photographs by the use of DCFH-DA fluorescence probe in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 50 μm. ( B ) Quantification of fluorescence intensity of ROS in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( C and D ) Measurement of MDA and GSH levels in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( E ) Representative JC-1 staining photographs in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Green fluorescence represents that MMP is low and JC-1 exists in the cytoplasm in the form of monomer. Red fluorescence represents that MMP is high and JC-1 polymer is formed. Scale bars, 50 μm. ( F ) Calculation of a ratio of green fluorescence and red fluorescence for quantifying MMP changes. ( G ) Detection of ATP in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. ( H ) TEM for investigating the changes in mitochondrial morphology in control macrophages, HG- and PA-induced macrophages, and HG- and PA-induced macrophages with CMA treatment. Scale bars, 500 nm. *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001 derived from one-way ANOVA followed by Tukey’s post hoc test.

    Article Snippet: After 24 h, 300 μL JC-1 staining solution (C2006; Beyotime) was added, with additional 20-min cultivation in the dark at 37°C.

    Techniques: Fluorescence, Control, Staining, Polymer, Morphology, Derivative Assay