Journal: Microorganisms

Article Title: Bacteroides fragilis Toxin Induces Sequential Proteolysis of E-Cadherin and Inflammatory Response in Mouse Intestinal Epithelial Cell Line

doi: 10.3390/microorganisms13040781

Figure Lengend Snippet: BFT induces E-cadherin cleavage in CMT93 cells. ( a ) Western blot analysis of CMT93, MSIE, CT26, YAMC, and B16 cells treated with serum-free media (SFM), rNTBF, or rETBF supernatant (1:20 dilution) for 3 h. E-cadherin was detected using monoclonal Ab specific for the endodomain of E-cadherin (clone C36). GAPDH, internal control; ( b ) Immunofluorescence imaging of CMT93 cells 3 h post-treatment. E-cadherin (green, clone C36) and nuclei (blue, DAPI) were observed with a confocal laser-scanning microscope (×1600); ( c ) Light microscope images of CMT93 cells observed under an inverted microscope (×40) 3 h post-treatment; ( d ) Putative BFT-induced cleavage site (arrow, red region) in human and mouse epithelial cell lines. Portions of the extracellular domain (green) and the transmembrane domain (blue) are shown. Amino acid sequences of E-cadherin were translated by Snapgene Viewer. Numbers, amino acid sequence.

Article Snippet: Amino acid sequences of E-cadherin based on the analyzed mRNA sequences were translated by Snapgene Viewer software (version 6.2.1, Dotmatics, Woburn, MA, USA).

Techniques: Western Blot, Control, Immunofluorescence, Imaging, Laser-Scanning Microscopy, Light Microscopy, Inverted Microscopy, Sequencing