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Journal: Oncology Letters
Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells
doi: 10.3892/ol.2018.9352
Figure Lengend Snippet: Control and normalization sequences for the microRNA microarray.
Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the
Techniques: Control, Microarray, Sequencing
Journal: Oncology Letters
Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells
doi: 10.3892/ol.2018.9352
Figure Lengend Snippet: miRNA chip overlay images. (A) Original image of two-channel miRNA microarray; PC-1.0 was labeled with red fluorescent Cy5 dye and PC-1 was labeled with green fluorescent Cy3 dye. (B) Original image of two-channel miRNA microarray; PC-1.0 was labeled with green fluorescent Cy5 dye and PC-1 was labeled with red fluorescent Cy3 dye. (C) Scatter plot of PC-1.0 and PC-1 data. miRNA, microRNA.
Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the
Techniques: Microarray, Labeling
Journal: Oncology Letters
Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells
doi: 10.3892/ol.2018.9352
Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction analysis of highly (PC-1.0) and weakly (PC-1) invasive and metastatic hamster pancreatic cancer cells. A total of 10 miRNAs were selected to verify the reliability of the miRNA microarray data. Of the 10 miRNAs, 6 were upregulated and 4 were downregulated. These results were very similar to the miRNA microarray data, supporting its reliability. Black bar, PC-1.0 cell line. White bar, PC-1 cell line. *P<0.05. miRNA, microRNA.
Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the
Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Microarray