Journal: International Journal of Molecular Sciences

Article Title: Runx3 Induces a Cell Shape Change and Suppresses Migration and Metastasis of Melanoma Cells by Altering a Transcriptional Profile

doi: 10.3390/ijms22042219

Figure Lengend Snippet: Microarray analysis uncovered an altered and specific transcriptional profile underlying the cell shape change and the suppression of the metastatic potential by Runx3. ( A ) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations assigned different repertoires of differentially expressed genes (DEGs) that were regulated by Runx3 in B16-F10 cells and, in the case of B16-F0 cells vs. B16-F10 cells, to the terms relevant to the actin cytoskeleton and adhesion. Each GO/KEGG annotation term is inscribed as a caption. The number of DEGs within a repertoire is described at x axis. The columns for the downregulated and the upregulated DEGs are shown in different colors as explained at the top of the figure., Down/Up by Runx3 indicates the appearance of DEGs in the case of B16-F10/Runx3 cells vs. mock control B16-F10 cells. Down/Up in B16-F0 indicates the appearance of DEGs in the case of B16-F0 cells vs. B16-F10 cells. Down/Up in common indicates the appearance of same DEGs in both cases. ( B ) Runx3 upregulated the expression of extracellular matrix (ECM) genes. The summary table indicates the gene names and their corresponding fold changes. ( C ) Runx3 regulated the expression of a list of DEGs that were inversely associated with an increase in the metastatic potential of B16-F10 cells compared to B16-F0 cells. The heatmap shows the gene names and their fold changes in the cases of B16-F10/Runx3 cells vs. mock control B16-F10 cells and B16-F0 cells vs. B16-F10 cells, respectively.

Article Snippet: SmartArray microarray chips (CapitalBio, Beijing, China) were prepared from 32K Mouse Genome Array version 4.0 ( http://www.Operon.com , accessed on 25 April 2011).

Techniques: Microarray, Control, Expressing

Journal: Oncology Letters

Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells

doi: 10.3892/ol.2018.9352

Figure Lengend Snippet: Control and normalization sequences for the microRNA microarray.

Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the SmartArray TM microarray system (CapitalBio Technology, Inc.).

Techniques: Control, Microarray, Sequencing

Journal: Oncology Letters

Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells

doi: 10.3892/ol.2018.9352

Figure Lengend Snippet: miRNA chip overlay images. (A) Original image of two-channel miRNA microarray; PC-1.0 was labeled with red fluorescent Cy5 dye and PC-1 was labeled with green fluorescent Cy3 dye. (B) Original image of two-channel miRNA microarray; PC-1.0 was labeled with green fluorescent Cy5 dye and PC-1 was labeled with red fluorescent Cy3 dye. (C) Scatter plot of PC-1.0 and PC-1 data. miRNA, microRNA.

Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the SmartArray TM microarray system (CapitalBio Technology, Inc.).

Techniques: Microarray, Labeling

Journal: Oncology Letters

Article Title: Differential expression profiles of microRNAs in highly and weakly invasive/metastatic pancreatic cancer cells

doi: 10.3892/ol.2018.9352

Figure Lengend Snippet: Reverse transcription-quantitative polymerase chain reaction analysis of highly (PC-1.0) and weakly (PC-1) invasive and metastatic hamster pancreatic cancer cells. A total of 10 miRNAs were selected to verify the reliability of the miRNA microarray data. Of the 10 miRNAs, 6 were upregulated and 4 were downregulated. These results were very similar to the miRNA microarray data, supporting its reliability. Black bar, PC-1.0 cell line. White bar, PC-1 cell line. *P<0.05. miRNA, microRNA.

Article Snippet: These probes were labeled onto a 75×25 mm chemically-modified plate using the SmartArray TM microarray system (CapitalBio Technology, Inc.).

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Microarray