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A slot blot apparatus placed on a raised surface (e.g., an upside-down 1 L plastic beaker) and attached to a vacuum line with a three-way stopcock air valve.

Journal: Bio-protocol

Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown

doi: 10.21769/BioProtoc.5627

Figure Lengend Snippet: A slot blot apparatus placed on a raised surface (e.g., an upside-down 1 L plastic beaker) and attached to a vacuum line with a three-way stopcock air valve.

Article Snippet: If using the Bio-Rad slot blot apparatus, two pieces of filter paper (equilibrated in 20 mM Tris-HCl) should be added below the nylon and nitrocellulose membranes ( ).

Techniques: Dot Blot

Onto the bottom piece of the slot blot apparatus, two pieces of filter paper should be placed only when using the Bio-Dot apparatus . For all slot blots, two pieces of positively charged nylon membrane, followed by 1 nitrocellulose membrane, should be placed before securing the top slot blot piece in place.

Journal: Bio-protocol

Article Title: Selective Isolation of TOP3B•mRNA Covalent Intermediates Using Denaturing Oligo-dT Pulldown

doi: 10.21769/BioProtoc.5627

Figure Lengend Snippet: Onto the bottom piece of the slot blot apparatus, two pieces of filter paper should be placed only when using the Bio-Dot apparatus . For all slot blots, two pieces of positively charged nylon membrane, followed by 1 nitrocellulose membrane, should be placed before securing the top slot blot piece in place.

Article Snippet: If using the Bio-Rad slot blot apparatus, two pieces of filter paper (equilibrated in 20 mM Tris-HCl) should be added below the nylon and nitrocellulose membranes ( ).

Techniques: Dot Blot, Membrane