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10X Genomics
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Journal: Cell Genomics
Article Title: A cell and transcriptome atlas of human arterial vasculature
doi: 10.1016/j.xgen.2025.101034
Figure Lengend Snippet: Adventitial fibroblast in human arteries (A) Featureplot of gene score of ECM- and collagen-related genes demonstrating enrichment in AdvFib. (B) Most specific adventitial (Adv) fibroblast marker, PI16 , RNA expression visualized on the RNA-seq UMAP (left), slide-seq spatial transcriptomics map (middle), and RNAscope (right), confirming the spatial identity of PI16-expressing fibroblasts. (C) UMAP visualization of canonical fibroblast marker genes with diffuse expression pattern, including S100A4 , PDGFRA , COL1A1 , and GLI1 , compared to additional new markers SFRP2 and MFAP5. (D) Relative specificity and sensitivities of commonly used and newly identified “fibroblast markers.” (E) Violin plots showing differential expression of key genes implicated in aneurysm FBN1 , TGFBR2 , LOX , FBLN1 , and MFAP5 expressed mostly in AdvFib. See also and .
Article Snippet:
Techniques: Marker, RNA Expression, RNA Sequencing, RNAscope, Expressing, Quantitative Proteomics