Journal: International Journal of Molecular Sciences

Article Title: Low Efficiency of Homology-Independent Targeted Integration for CRISPR/Cas9 Correction in the Vicinity of the SLC26A4 c.919-2A>G Variant

doi: 10.3390/ijms26114980

Figure Lengend Snippet: HITI-mediated correct integration with distinct query and the corresponding NGS data. ( a ) Schematic of the wild-type and correct integration genomes, as well as the forward/reverse primers (blue/orange arrows) and distinguished queries (Query WT vs. Query CI framed by black lines). Red arrow: Cas9 cutting site. ( b ) The sequences before and after correct integration (Query WT vs. Query CI in framed region) and the refined NGS reads of SLC-2 mapped onto SLC26A4 genomic region that harbors Query WT or Query CI (framed region in lower-left panel), accompanied by the zoom-in view and pairwise alignment for the Query WT and Query CI segment (right panel). The Cas9/gRNA target sequence is shown in grey. The PAM sequence is underlined.

Article Snippet: Later, Takeda et al. [ ] demonstrated that transuterine gene transfer of SLC26A4 cDNA into the otocysts of SLC26A4 -deficient mice could restore hearing and vestibular functions.

Techniques: Sequencing

Journal: International Journal of Molecular Sciences

Article Title: Low Efficiency of Homology-Independent Targeted Integration for CRISPR/Cas9 Correction in the Vicinity of the SLC26A4 c.919-2A>G Variant

doi: 10.3390/ijms26114980

Figure Lengend Snippet: The candidate sgRNAs with putative targeting sites surrounding the SLC26A4 c.919-2A>G variant. The sgRNA is shown with a 5′ protospacer (light blue) and a 3′ PAM motif (indigo).

Article Snippet: Later, Takeda et al. [ ] demonstrated that transuterine gene transfer of SLC26A4 cDNA into the otocysts of SLC26A4 -deficient mice could restore hearing and vestibular functions.

Techniques: Variant Assay