Journal: Journal of Gastrointestinal Oncology

Article Title: TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma

doi: 10.21037/jgo-2025-1-1084

Figure Lengend Snippet: TRNP1 knockdown affects the proliferation, invasion, migration and apoptosis of HCC. (A) The protein expression of TRNP1 in the HCC cell lines. (B) Images (left panel) and quantified analysis (right panel) of TRNP1 expression detected by western blot in Sk-hep1 and YY8103 cells. (C) Cell proliferation of TRNP1 -NC, TRNP1 -sh1, and TRNP1 -sh2 groups in Sk-hep1 and YY8103 cells was evaluated by CCK-8 assay. (D) Images (left panel) and quantified analysis (right panel) of clone formation assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (E,F) Images (left panel) and quantified analysis (right panel) of transwell migration and invasion assays (stained with 0.5% crystal violet) in Sk-hep1 and YY8103 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. Scale bars: 100 µm. (G) Flow cytometric assays detecting the effects of TRNP1 knockdown on apoptosis in HCC cells (left panel) and statistical results (right panel). (H) Images (left panel) and quantified analysis (right panel) of apoptosis-related proteins expression detected by western blot in Sk-hep1 and YY8103 cells. (I) Expression of stem cell genes in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. CCK-8, Cell Counting Kit-8; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HCC, hepatocellular carcinoma; NC, negative control; TRNP1 , TMF1-regulated nuclear protein 1.

Article Snippet: In this study, the human HCC cell lines Sk-hep1, Hep3B, and HepG2 were obtained from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Migration, Expressing, Western Blot, CCK-8 Assay, Staining, Cell Counting, Negative Control

Journal: Journal of Gastrointestinal Oncology

Article Title: TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma

doi: 10.21037/jgo-2025-1-1084

Figure Lengend Snippet: TRNP1 promotes HCC proliferation in vivo . The representative images (A), tumor weights (B) and tumor growth curves (C) of xenograft tumors from Sk-hep1 cells with TRNP1 -NC, TRNP1 -sh1 and TRNP1 -sh2. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 cells with/without TRNP1 knockdown. (E) Representative images (left panel) and quantitative analysis (right panel) of IHC staining of PCNA in xenografts of Sk-hep1 cells with TRNP1 -NC, and TRNP1 -sh2. Scale bars: 50 µm. *, P<0.05; **, P<0.01. HCC, hepatocellular carcinoma; IHC, immunohistochemistry; NC, negative control; PCNA, proliferating cell nuclear antigen; TRNP1 , TMF1-regulated nuclear protein 1.

Article Snippet: In this study, the human HCC cell lines Sk-hep1, Hep3B, and HepG2 were obtained from the American Type Culture Collection (ATCC).

Techniques: In Vivo, Western Blot, Knockdown, Immunohistochemistry, Negative Control

Journal: Journal of Gastrointestinal Oncology

Article Title: TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma

doi: 10.21037/jgo-2025-1-1084

Figure Lengend Snippet: TRNP1 affects the malignant phenotype by targeting the c-Kit/STAT3 signaling pathway. (A) Volcano plot of differentially expressed genes in Sk-hep1 cells with/without TRNP1 knockdown. (B) KEGG enrichment analysis of differentially expressed genes. (C) Expression of relative KIT mRNA in Sk-hep1 cells with/without TRNP1 knockdown. (D) Western blot images (left) and quantitative analysis (right) of various markers in Sk-hep1 and YY8103 cells with/without TRNP1 knockdown. (E) Images (left panel) and quantified analysis (right panel) of EMT markers (E-cadherin, N-cadherin) detected by western blot in Sk-hep1 and YY8103 cells. (F,G) Representative images (left) and quantitative analysis (right) of IHC staining of c-Kit, P-STAT3 and E-cadherin, N-cadherin in xenografts of Sk-hep1 cells with/without TRNP1 knockdown. Scale bars: 50 µm. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. EMT, epithelial-mesenchymal transition; FC, fold change; FDR, false discovery rate; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IHC, immunohistochemistry; KEGG, Kyoto Encyclopedia of Genes and Genomes; mRNA, messenger RNA; NC, negative control; STAT3, signal transducers and activators of transcription 3; TRNP1 , TMF1-regulated nuclear protein 1.

Article Snippet: In this study, the human HCC cell lines Sk-hep1, Hep3B, and HepG2 were obtained from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Expressing, Western Blot, Immunohistochemistry, Negative Control

Journal: Journal of Gastrointestinal Oncology

Article Title: TRNP1 regulates tumorigenesis and enhances immunotherapy response via c-Kit/STAT3 signaling in hepatocellular carcinoma

doi: 10.21037/jgo-2025-1-1084

Figure Lengend Snippet: TRNP1 knockdown enhances the therapeutic response of HCC to PD-1 blockade. (A) The correlation between the gene expression of TRNP1 and PDCD1 in human HCC samples using the GEPIA web tool. (B) Western blot images (left) and quantitative analysis (right) of TRNP1 knockdown in Hepa 1-6 cells are shown below. The representative images (C), tumor weight (D) and tumor growth (E) of xenograft tumors from Hepa 1-6 cells with NC and sh TRNP1 with/without anti-PD-1. (F) Lollipop graph illustrates the correlation between immune infiltration and TRNP1 expression. (G) The proportion of M1 and M2 macrophages in NC and TRNP1 knockdown Hepa 1–6 subcutaneous tumors was detected by flow cytometry, along with statistical data. (H) Volcano plot of ANXA1 expression in Sk-hep1 cells with or without TRNP1 knockdown. q-PCR (I) and ELISA (J) analyses show the expression of ANXA1 in mouse subcutaneous tumor tissues. ns, P>0.05; *, P<0.05; **, P<0.01; ***, P<0.001; ****, P<0.0001. ANXA1, annexin A1; ELISA, enzyme-linked immunosorbent assay; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; GEPIA, Gene Expression Profiling Interactive Analysis; HCC, hepatocellular carcinoma; NC, negative control; PD-1, programmed cell death protein 1; q-PCR, quantitative real-time polymerase chain reaction; TRNP1 , TMF1-regulated nuclear protein 1.

Article Snippet: In this study, the human HCC cell lines Sk-hep1, Hep3B, and HepG2 were obtained from the American Type Culture Collection (ATCC).

Techniques: Knockdown, Clinical Proteomics, Gene Expression, Western Blot, Expressing, Flow Cytometry, Enzyme-linked Immunosorbent Assay, Negative Control, Real-time Polymerase Chain Reaction