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Sk Mel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sk mel 28 cell lines  (Procell Inc)
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PDA-Ce6-R837 NPs induce PTT and PDT effects in melanoma cells. (A) Fluorescence imaging demonstrating the cellular uptake of PDA-Ce6-R837 <t>NPs</t> <t>by</t> <t>SK-Mel-28</t> and A375 cells. Cell viability of SK-Mel-28 (B) and A375 (C) cells following treatment with increasing concentrations of PDA-Ce6-R837 NPs, as assessed by the CCK-8 assay. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Control group. CCK-8 analysis of the effects of PDA-Ce6-R837 NPs at different concentrations on the viability of SK-Mel-28 (D) and A375 (E) cells in the presence or absence of laser irradiation. Intracellular ROS production in SK-Mel-28 (F) and A375 (G) cells after PDA-Ce6-R837 NPs treatment with or without laser irradiation. (H) Quantitative analysis of ROS levels. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Sk Mel 28 Cell Lines, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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PDA-Ce6-R837 NPs induce PTT and PDT effects in melanoma cells. (A) Fluorescence imaging demonstrating the cellular uptake of PDA-Ce6-R837 <t>NPs</t> <t>by</t> <t>SK-Mel-28</t> and A375 cells. Cell viability of SK-Mel-28 (B) and A375 (C) cells following treatment with increasing concentrations of PDA-Ce6-R837 NPs, as assessed by the CCK-8 assay. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Control group. CCK-8 analysis of the effects of PDA-Ce6-R837 NPs at different concentrations on the viability of SK-Mel-28 (D) and A375 (E) cells in the presence or absence of laser irradiation. Intracellular ROS production in SK-Mel-28 (F) and A375 (G) cells after PDA-Ce6-R837 NPs treatment with or without laser irradiation. (H) Quantitative analysis of ROS levels. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Emem Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sk mel  (ATCC)
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PDA-Ce6-R837 NPs induce PTT and PDT effects in melanoma cells. (A) Fluorescence imaging demonstrating the cellular uptake of PDA-Ce6-R837 <t>NPs</t> <t>by</t> <t>SK-Mel-28</t> and A375 cells. Cell viability of SK-Mel-28 (B) and A375 (C) cells following treatment with increasing concentrations of PDA-Ce6-R837 NPs, as assessed by the CCK-8 assay. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Control group. CCK-8 analysis of the effects of PDA-Ce6-R837 NPs at different concentrations on the viability of SK-Mel-28 (D) and A375 (E) cells in the presence or absence of laser irradiation. Intracellular ROS production in SK-Mel-28 (F) and A375 (G) cells after PDA-Ce6-R837 NPs treatment with or without laser irradiation. (H) Quantitative analysis of ROS levels. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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human melanoma cell line sk mel 2  (Procell Inc)
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(A) Six predicted cER genes were <t>investigated</t> <t>in</t> <t>SK-mel-2</t> cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.
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sk mel 103  (ATCC)
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(A) Six predicted cER genes were <t>investigated</t> <t>in</t> <t>SK-mel-2</t> cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.
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sk mel 3  (ATCC)
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(A) Six predicted cER genes were <t>investigated</t> <t>in</t> <t>SK-mel-2</t> cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.
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PDA-Ce6-R837 NPs induce PTT and PDT effects in melanoma cells. (A) Fluorescence imaging demonstrating the cellular uptake of PDA-Ce6-R837 NPs by SK-Mel-28 and A375 cells. Cell viability of SK-Mel-28 (B) and A375 (C) cells following treatment with increasing concentrations of PDA-Ce6-R837 NPs, as assessed by the CCK-8 assay. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Control group. CCK-8 analysis of the effects of PDA-Ce6-R837 NPs at different concentrations on the viability of SK-Mel-28 (D) and A375 (E) cells in the presence or absence of laser irradiation. Intracellular ROS production in SK-Mel-28 (F) and A375 (G) cells after PDA-Ce6-R837 NPs treatment with or without laser irradiation. (H) Quantitative analysis of ROS levels. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: RSC Advances

Article Title: Photo-immunotherapeutic PDA-Ce6-R837 nanoparticles enhance the induction of immunogenic responses in melanoma cells

doi: 10.1039/d6ra01921h

Figure Lengend Snippet: PDA-Ce6-R837 NPs induce PTT and PDT effects in melanoma cells. (A) Fluorescence imaging demonstrating the cellular uptake of PDA-Ce6-R837 NPs by SK-Mel-28 and A375 cells. Cell viability of SK-Mel-28 (B) and A375 (C) cells following treatment with increasing concentrations of PDA-Ce6-R837 NPs, as assessed by the CCK-8 assay. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, compared with Control group. CCK-8 analysis of the effects of PDA-Ce6-R837 NPs at different concentrations on the viability of SK-Mel-28 (D) and A375 (E) cells in the presence or absence of laser irradiation. Intracellular ROS production in SK-Mel-28 (F) and A375 (G) cells after PDA-Ce6-R837 NPs treatment with or without laser irradiation. (H) Quantitative analysis of ROS levels. Values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human melanoma A375 and SK-Mel-28 cell lines were obtained from Procell (Wuhan, China).

Techniques: Fluorescence, Imaging, CCK-8 Assay, Control, Irradiation

PDA-Ce6-R837 NPs mediated phototherapy suppresses melanoma cells migration and invasion. (A) Representative images of wound healing assays in SK-Mel-28 cells treated with PDA-Ce6-R837 NPs with or without laser irradiation at 0 h and 24 h. (B) Quantitative analysis of wound closure in SK-Mel-28 cells under the indicated treatment conditions. (C) Representative images of wound healing assays in A375 cells treated with PDA-Ce6-R837 NPs with or without laser irradiation at 0 h and 24 h. (D) Quantitative analysis of wound closure in A375 cells under the indicated treatment conditions. (E) Representative images of Transwell invasion assays showing the invasive behavior of melanoma cells under different treatments. (F) Quantification of invaded SK-Mel-28 and A375 cells following PDA-Ce6-R837 NPs mediated phototherapy. All values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: RSC Advances

Article Title: Photo-immunotherapeutic PDA-Ce6-R837 nanoparticles enhance the induction of immunogenic responses in melanoma cells

doi: 10.1039/d6ra01921h

Figure Lengend Snippet: PDA-Ce6-R837 NPs mediated phototherapy suppresses melanoma cells migration and invasion. (A) Representative images of wound healing assays in SK-Mel-28 cells treated with PDA-Ce6-R837 NPs with or without laser irradiation at 0 h and 24 h. (B) Quantitative analysis of wound closure in SK-Mel-28 cells under the indicated treatment conditions. (C) Representative images of wound healing assays in A375 cells treated with PDA-Ce6-R837 NPs with or without laser irradiation at 0 h and 24 h. (D) Quantitative analysis of wound closure in A375 cells under the indicated treatment conditions. (E) Representative images of Transwell invasion assays showing the invasive behavior of melanoma cells under different treatments. (F) Quantification of invaded SK-Mel-28 and A375 cells following PDA-Ce6-R837 NPs mediated phototherapy. All values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human melanoma A375 and SK-Mel-28 cell lines were obtained from Procell (Wuhan, China).

Techniques: Migration, Irradiation

PDA-Ce6-R837 NPs mediated phototherapy induces apoptosis in melanoma cells. (A) Representative flow cytometry plots showing apoptosis in SK-Mel-28 and A375 cells following different treatments. Quantitative analysis of apoptotic rates in SK-Mel-28 (B) and A375 (C) cells under indicated conditions. All values represent the mean ± SD, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: RSC Advances

Article Title: Photo-immunotherapeutic PDA-Ce6-R837 nanoparticles enhance the induction of immunogenic responses in melanoma cells

doi: 10.1039/d6ra01921h

Figure Lengend Snippet: PDA-Ce6-R837 NPs mediated phototherapy induces apoptosis in melanoma cells. (A) Representative flow cytometry plots showing apoptosis in SK-Mel-28 and A375 cells following different treatments. Quantitative analysis of apoptotic rates in SK-Mel-28 (B) and A375 (C) cells under indicated conditions. All values represent the mean ± SD, n = 3. ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human melanoma A375 and SK-Mel-28 cell lines were obtained from Procell (Wuhan, China).

Techniques: Flow Cytometry

PDA-Ce6-R837 NPs mediated phototherapy induces ICD in melanoma cells. Immunofluorescence staining of CRT in SK-Mel-28 (A) and A375 (B) cells under basal conditions or following treatment with PDA-Ce6-R837 NPs with or without laser irradiation. (C) Quantification of membrane-associated CRT fluorescence intensity after treatment. Measurement of extracellular ATP (D) and HMGB1 (E) release in response to PDA-Ce6-R837 NPs with or without laser irradiation. Scale bar = 50 µm. All values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Journal: RSC Advances

Article Title: Photo-immunotherapeutic PDA-Ce6-R837 nanoparticles enhance the induction of immunogenic responses in melanoma cells

doi: 10.1039/d6ra01921h

Figure Lengend Snippet: PDA-Ce6-R837 NPs mediated phototherapy induces ICD in melanoma cells. Immunofluorescence staining of CRT in SK-Mel-28 (A) and A375 (B) cells under basal conditions or following treatment with PDA-Ce6-R837 NPs with or without laser irradiation. (C) Quantification of membrane-associated CRT fluorescence intensity after treatment. Measurement of extracellular ATP (D) and HMGB1 (E) release in response to PDA-Ce6-R837 NPs with or without laser irradiation. Scale bar = 50 µm. All values represent the mean ± SD, n = 3. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

Article Snippet: Human melanoma A375 and SK-Mel-28 cell lines were obtained from Procell (Wuhan, China).

Techniques: Immunofluorescence, Staining, Irradiation, Membrane, Fluorescence

(A) Six predicted cER genes were investigated in SK-mel-2 cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.

Journal: PLOS Computational Biology

Article Title: CASER: A semi-supervised model with multi-omics data integration prioritizes cancer-associated epigenetic regulator genes

doi: 10.1371/journal.pcbi.1014253

Figure Lengend Snippet: (A) Six predicted cER genes were investigated in SK-mel-2 cell line ( n = 4). (B) Six predicted cER genes were investigated in Caki-1 cell line ( n = 4). Statistical analysis was performed between si-NC and si-Genes. P -values are calculated by One-way analysis of variance followed by Dunnett’s corrections and indicated by star symbols, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, P > 0.05.

Article Snippet: The human melanoma cell line SK-mel-2, clear cell renal cell carcinoma cell line Caki-1, breast cancer cell line MDA-MB-231, and prostate cancer cell line LNCaP, along with their corresponding complete median, were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: