Journal: Journal of Inflammation Research

Article Title: Protective Effects of CR6-Interacting Factor 1 Against Angiotensin II-Induced Atrial Fibrillation by Regulating the SIRT1/eNOS Signaling Pathway and Cardiomyocyte Remodeling

doi: 10.2147/JIR.S596132

Figure Lengend Snippet: Overexpression of CRIF1 activates the SIRT1/eNOS pathway in cardiomyocytes treated with Ang II. HL-1 cells were transfected with pcDNA3.1-CRIF1 (OE-CRIF1) or vector for 24 h, and then treated with Ang II (1 μM) for an additional 24 h. The mRNA expression levels of ( A ) CRIF1, ( B ) SIRT1 and ( C ) eNOS were evaluated using RT-qPCR. HL-1 cells were transfected with pcDNA3.1-CRIF1 (OE-CRIF1) or vector for 24 h, and were then cotreated with a SIRT1 inhibitor EX527 (10 μM) and Ang II (1 μM) for further 24 h. ( D ) The cell apoptosis was assessed by staining with TUNEL and DAPI, and observed under a fluorescence microscope (200 X). ( E ) Apoptosis was quantified by calculating TUNEL positive cells (normalized to DAPI-stained cells). Data are presented as mean ± SD in triplicates. ***P<0.001 vs control group; ### P<0.001 vs Ang II+Vector group; $$$ P<0.001 vs OE-CRIF1 group.

Article Snippet: HL-1 cells were treated with 1 μM Ang II for 24 h to generate an in vitro AF model. To explore the role of SIRT1, the SIRT1 inhibitor EX527 (10 μM, HY-15452, MedChemExpress) was used to treat HL-1 cells.

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Quantitative RT-PCR, Staining, TUNEL Assay, Fluorescence, Microscopy, Control

Journal: Journal of Inflammation Research

Article Title: Protective Effects of CR6-Interacting Factor 1 Against Angiotensin II-Induced Atrial Fibrillation by Regulating the SIRT1/eNOS Signaling Pathway and Cardiomyocyte Remodeling

doi: 10.2147/JIR.S596132

Figure Lengend Snippet: Schematic diagram illustrating the role of CRIF1 in angiotensin II–induced atrial remodeling and the protective effects of its overexpression. Chronic angiotensin II (Ang II) infusion, combined with atrial fibrillation (AF) induction, leads to increased AF duration and inducibility, elevated markers of cardiac injury (CK-MB, LDH) and hypertrophy (ANP/BNP). This pathological state is associated with enhanced CRIF1 expression. Overexpression of CRIF1 activates the SIRT1/eNOS signaling pathway, which mediates cardioprotective effects. These effects include suppression of inflammatory cytokines, diminished reactive oxygen species (ROS) accumulation, restoration of antioxidant enzyme activity, and restoration of nitric oxide production. Consequently, CRIF1 overexpression reduces cardiotoxicity, apoptosis, and Ang II–induced hypertrophy. These beneficial effects are dependent on SIRT1 signaling.

Article Snippet: HL-1 cells were treated with 1 μM Ang II for 24 h to generate an in vitro AF model. To explore the role of SIRT1, the SIRT1 inhibitor EX527 (10 μM, HY-15452, MedChemExpress) was used to treat HL-1 cells.

Techniques: Over Expression, Expressing, Activity Assay

Journal: Chinese Medicine

Article Title: Calculus Bovis ameliorates primary sclerosing cholangitis via a dual-pronged mechanism restoring bile acid and lipid homeostasis in the gut-liver axis

doi: 10.1186/s13020-026-01441-w

Figure Lengend Snippet: CB upregulates intestinal and hepatic FXR and PPARα via the SIRT1-PGC-1α axis. A Volcano plot of liver transcriptome data highlighting upregulation of Fxr and Pparα by CB. B Hepatic mRNA levels of nuclear receptors. C Representative western blots of hepatic nuclear receptor proteins. D Hepatic mRNA levels of Shp (FXR target) and Fabp1 (PPARα target). E Ileum mRNA levels and F representative western blots of nuclear receptors. G Ileum mRNA levels of Fgf15 (FXR target) and Fabp1 (PPARα target). H Screening of upstream regulators from transcriptome data identified SIRT1 and PGC-1α as top candidates. I mRNA and J protein levels of SIRT1 and PGC-1α in liver and intestine. All mRNA levels were determined by RT-qPCR and normalized to Hprt1 . Protein levels were analyzed by western blotting and normalized to Gapdh. Data are presented as mean ± SEM (n = 4–6). * p < 0.05, ** p < 0.01, *** p < 0.001 vs . Control + Veh; # p < 0.05, ## p < 0.01, ### p < 0.001 vs . DDC + Veh

Article Snippet: SIRT1 inhibitor SIRT1-IN-1 (# HY136199 ) and FXR antagonist Z-guggulsterone (#HY-110066) were purchased from Med Chem Express (Shanghai, China).

Techniques: Western Blot, Quantitative RT-PCR, Control

Journal: Chinese Medicine

Article Title: Calculus Bovis ameliorates primary sclerosing cholangitis via a dual-pronged mechanism restoring bile acid and lipid homeostasis in the gut-liver axis

doi: 10.1186/s13020-026-01441-w

Figure Lengend Snippet: In vitro validation of the SIRT1-PGC-1α-FXR/PPARα axis as the mechanistic core for CB's action. A Schematic overview of the in vitro experimental design. B – E HepG2 and Caco-2 cells were treated with DDC and increasing concentrations of CB-containing serum (CB-S). B , D Western blots of SIRT1, PGC-1α, FXR, and PPARα in HepG2 B and Caco-2 D cells. C , E RT-qPCR analysis of indicated genes in HepG2 C and Caco-2 E cells. F – I Cells pre-treated with SIRT1 inhibitor SIRT1-IN-1 before DDC + 20% CB-S. F , H Western blots of SIRT1, PGC-1α, FXR, and PPARα in HepG2 F and Caco-2 H cells. (G, I) RT-qPCR analysis of indicated genes in HepG2 G and Caco-2 I cells. J – O Cells treated with DDC + 20% CB-S, and FXR antagonist (Z-guggulsterone, Z-gug) and/or PPARα antagonist (MK-886). (J, M) RT-qPCR analysis of FXR and PPARα mRNA levels in HepG2 J and Caco-2 M cells. K , N Western blots of FXR and PPARα in HepG2 K and Caco-2 N cells. L , O RT-qPCR analysis of indicated genes in HepG2 L and Caco-2 O cells. P – S DDC-injured HepG2 and Caco-2 cells were treated with ABA or bilirubin in the presence or absence of serum. P , R RT-qPCR analysis of indicated genes in HepG2 P and Caco-2 R cells. Q , S Western blots of SIRT1, PGC-1α, FXR, and PPARα in HepG2 Q and Caco-2 S cells. All mRNA levels were determined by RT-qPCR and normalized to GAPDH . Protein levels were analyzed by western blotting and normalized to GAPDH. Data are presented as mean ± SEM (n = 3–4). *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: SIRT1 inhibitor SIRT1-IN-1 (# HY136199 ) and FXR antagonist Z-guggulsterone (#HY-110066) were purchased from Med Chem Express (Shanghai, China).

Techniques: In Vitro, Biomarker Discovery, Western Blot, Quantitative RT-PCR

Journal: Chinese Medicine

Article Title: Calculus Bovis ameliorates primary sclerosing cholangitis via a dual-pronged mechanism restoring bile acid and lipid homeostasis in the gut-liver axis

doi: 10.1186/s13020-026-01441-w

Figure Lengend Snippet: Schematic diagram illustrating the proposed mechanism by which CB ameliorates PSC. CB and its bioactive BA components alleviate PSC by restoring bile acid and lipid homeostasis via the gut-liver axis through a dual mechanism: (1) transcriptional upregulation of FXR and PPARα in hepatocytes and enterocytes through activation of the SIRT1-PGC-1α axis; and (2) direct ligand-dependent activation of FXR and PPARα in these cells. This concerted action enhances the transcription of genes involved in bile acid detoxification, transport, and fatty acid β-oxidation, thereby reducing hepatic injury, fibrosis, and intestinal barrier damage

Article Snippet: SIRT1 inhibitor SIRT1-IN-1 (# HY136199 ) and FXR antagonist Z-guggulsterone (#HY-110066) were purchased from Med Chem Express (Shanghai, China).

Techniques: Activation Assay

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: SIRT1 deficiency promotes senescence in trophoblast Cells (A) Western blot validates shSIRT1 interference efficiency in HTR8/SVneo cells (B) Western blot detection of the protein levels of p16 and p21; n = 3. (C) SA-β-gal staining of HTR8/SVneo cells; Scale bars: 200 μm; n = 3. (D–F) Levels of (D) IL-6, (E) IL-1αand (F) IL-1β concentration in the cells were measured using the corresponding detection kits; n = 3 independent experiments. Two-tailed t-test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Western Blot, Staining, Concentration Assay, Two Tailed Test

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: SIRT1 alleviates PE-like symptoms in the mouse model (A) Schematic illustration of the experimental design. (B) Representative images of the fetuses of the Control, L-NAME, and L-NAME + SRT2104 groups; Scale bars:1 cm. (C) Systolic blood pressure of pregnant mice in control (n = 6 dam), L-NAME (n = 7 dams), and L-NAME + SRT2104 group (n = 7 dams). (D–F) Fetal weight, (E) crown-rump length,and (F) placental weight at E18.5 in different groups; n = 85 fetuses from 6 dams in the control group, n = 98 fetuses from 7 dams in the L-NAME group, and n = 81 fetuses from 7 dams in the L-NAME + SRT2104 group. (G) H&E staining of placental sections at E18.5. The LZ and JZ areas and the Lz/Jz ratio were quantified; Scale bars: 1000 μm (upper panel), 200um (lower panel); n = 3. (H) H&E staining of maternal kidney sections at E18.5, and measurement of Bowman space; Scale bars: 200 μm; n = 3. LZ, Labyrinth zone; JZ, Junction zone. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Control, Staining, Comparison

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: SIRT1 rescues placental senescence in the PE mouse model (A) Western blot detection of the protein levels of p16, p21 and SIRT1 in E18.5 mouse placentas from different groups; n = 3. (B) SA-β-gal staining in E18.5 mouse placentas from different groups. Scale bars: 1,000 μm, n = 3 (C,D) Representative immunofluorescence staining of (C) SIRT1(red) and p16 (green) or (D) SIRT1 (red) and p21 (green), in E18.5 placentas from different groups; Nuclei were counterstained with DAPI (blue). Scale bars: 1,000 μm; n = 3. (E–G) Levels of (E) IL-6, (F) IL-1α and (G) IL-1β concentration in the E18.5 mouse placentas from different groups were measured using the corresponding detection kits; n = 3, one-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Western Blot, Staining, Immunofluorescence, Concentration Assay, Comparison

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: Elimination of senescent cells alleviates SIRT1 deficiency–induced dysfunction and SASP (A–E) HTR-8/SVneo cells were transfected with shSIRT1 and subsequently treated with PCC1 (5 μM) for 24 h (A) SA-β-gal staining cells; Scale bars: 200 μm; n = 3. (B) Western blot analysis of the protein levels of p16, p21 and SIRT1 in cells; n = 3. (C–E) Levels of (C) IL-6 (D) IL-1α and (E) IL-1β concentration in the cells were measured using corresponding detection kits; n = 3. (F) HTR-8/SVneo cells were transfected with shSIRT1 and then treated with PCC1 (5 μM) or SRT1720 (0.4 uM) for various time periods. Cell viability was measured by CCK8; n = 6. (G–I) HTR-8/SVneo cells were transfected with shSIRT1 and subsequently treated with PCC1 (5 μM) or SRT1720 (0.4 uM) for 24 h. (G) EdU staining; Scale bars: 400 μm; n = 3 (H) Matrigel Transwell assay; Scale bars: 200 μm; n = 3. (I) Wound-healing assay; Scale bars: 400 μm; n = 3. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Transfection, Staining, Western Blot, Concentration Assay, Transwell Assay, Wound Healing Assay, Comparison

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: Iron metabolism dysregulation correlates with SIRT1 deficiency (A) Volcano plot of the significant differences in gene expression levels between groups. Genes showing the greatest differences were analyzed by (B) GO and (C) KEGG analysis. (D) Heatmap depicting the expression levels of representative genes associated with “intracellular iron ion homeostasis”. (E) Western blot detection of the protein levels of FTH, GPX4, SLC7A11, FPN1 and TFR; n = =3. (F–H) Measurement of intracellular (F) iron concentration, (G) GSH concentration, and (H) MDA concentration using corresponding detection kits; n = 3. (I) Measuring cellular lipid peroxidation by fluorescence microscopy using the C11 BODIPY 581/591 fluorescent probe. Total C11 BODIPY 581/591 (red), oxidized C11 BODIPY 581/591 (green), DAPI (blue) stained nucleus; Scale bar: 100 μm; n = 3. (J) Intracellular Fe 2+ was measured by fluorescence microscopy using the FerroOrange fluorescent probe. FerroOrange (red) stains Fe 2+ and DAPI (blue) stains the nucleus; Scale bar: 200 μm; n = 3. (K) Measuring mitochondrial membrane potential using the JC-1 fluorescent probe. JC-1 aggregates (red), JC-1 monomers (green) and DAPI (blue) stained nucleus; Scale bar: 100 μm; n = 3. (L) Flow cytometry assay for measuring ROS by staining with DCFH-DA, normalized by the number of cells uploaded; n = 3 (M) Transmission electron microscopy images of organelles. Scale bars: 1 µm (left panel) and 500 nm (right panel); N, nucleus; Mi, mitochondria. N = 3, two-tailed t-test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Gene Expression, Expressing, Western Blot, Concentration Assay, Fluorescence, Microscopy, Staining, Membrane, Flow Cytometry, Transmission Assay, Electron Microscopy, Two Tailed Test

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: SIRT1 regulates iron metabolism and senescence in trophoblast cells via p53 deacetylation (A) Reciprocal Co-IP of SIRT1 and p53 in HTR8/SVneo cells. (B) Western blot analysis of ac-p53, p53, and SIRT1; n = 3. (C) Western blot validates si-p53 interference efficiency. (D) Western blot detection of the protein levels of ac-p53, p53, SLC7A11, FPN1, TFR, SIRT1, p16, FTH, p21 and GPX4; n = 3. (E) After treatment, fluorescence images of total C11 BODIPY 581/591 (red) and oxidized C11 BODIPY 581/591 (green) in HTR-8/SVneo cells as indicated; Scale bar: 100 μm; n = 3. (F) After treatment, fluorescence images of Fe 2+ (red) in HTR-8/SVneo cells as indicated; Scale bar: 200 μm; n = 3. (G–I) Levels of (G) IL-6, (H) IL-1α and (I) IL-1β concentration in cells were measured using corresponding detection kits; n = 3. One-way ANOVA and Tukey’s multiple comparison test. All data are presented as the means ± SEM.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: Co-Immunoprecipitation Assay, Western Blot, Fluorescence, Concentration Assay, Comparison

Journal: Frontiers in Aging

Article Title: The SIRT1–p53 axis drives a ferro-aging-like program and aggravates trophoblast dysfunction in preeclampsia

doi: 10.3389/fragi.2026.1838730

Figure Lengend Snippet: Schematic illustration of the mechanism by which SIRT1 drives a positive feedback loop between iron metabolism and senescence in trophoblasts.

Article Snippet: Lentiviral vectors carrying short hairpin RNA (shRNA) targeting human SIRT1 (GV248) and a negative control shRNA (sh-NC) were purchased from GeneChem (Shanghai, China).

Techniques: